Novel device prototyping for endoscopic cell sheet transplantation using a three-dimensional printed simulator

• Main Authors: Hiroaki Osada, Wen-Jin Ho, Hideki Yamashita, Kazuhiro Yamazaki, Tadashi Ikeda, Kenji Minatoya, Hidetoshi Masumoto
• Format: Article
• Language: English
• Published: Elsevier 2020-12-01
• Series: Regenerative Therapy
• Subjects: Cardiac regenerative medicine; Cell sheet; Cell therapy; Minimally-invasive surgery; Simulator study
• Online Access: http://www.sciencedirect.com/science/article/pii/S2352320420300833

Introduction: Considering higher risks of candidates for cardiac regenerative therapy with compromised cardiac function, it is anticipated to develop less invasive surgical procedures. In the present study, we aimed to develop a prototype of totally endoscopic cell sheet delivery device and evaluate the surgical technique for epicardial cell sheet placement using three-dimensional (3D) printed simulators based on human computed tomography data. Methods: We designed an endoscopic cell sheet delivery device with outer and inner frame with self-expandable applicator which can be opened in thoracic cavity. We launched spout line to provide liquids on the applicator surface and tension line to gently bend the applicator dorsally. We prepared human mesenchymal stem cell (MSC) sheets and compared wet/dry conditions of 3D printed heart/porcine heart and applicator to identify suitable conditions for cell sheet transplantation. Finally we validated the feasibility of endoscopic transplantation to anterior and lateral wall of left ventricle using 3D printed simulators. Results: Moist condition of both 3D printed heart/porcine heart surface and applicator at transplantation yielded highest successful rate (100%, p = 0.0197). For both endoscopic transplantation sites, MSC sheets were successfully deployed. The procedure duration was 157 ± 23 s for anterior wall and 123 ± 13 s for the lateral wall in average, respectively. Conclusions: We developed a novel prototype of endoscopic cell sheet delivery device for minimally-invasive cardiac regenerative therapy utilizing a 3D printed simulator. The commercialization of the prototype may provide a safe minimally-invasive method to deliver potential cardiac regenerative therapy in the future.