Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows
Background: The objectives of this study were to identify loci, positional candidate genes, gene-sets, and pathways associated with spontaneous abortion (SA) in cattle and compare these results with previous human SA studies to determine if cattle are a good SA model for humans. Pregnancy was determ...
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doaj-102d5b05e503414798dfeef44f55923a2020-11-25T01:15:07ZengMDPI AGGenes2073-44252019-11-01101295410.3390/genes10120954genes10120954Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous CowsKayleen F. Oliver0Alexandria M. Wahl1Mataya Dick2Jewel A. Toenges3Jennifer N. Kiser4Justine M. Galliou5Joao G.N. Moraes6Gregory W. Burns7Joseph Dalton8Thomas E. Spencer9Holly L. Neibergs10Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USADepartment of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USADepartment of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USADepartment of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USADepartment of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USADepartment of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USAAnimal Sciences Research Center, Division of Animal Sciences, University of Missouri, Columbia, MO S158A, USAAnimal Sciences Research Center, Division of Animal Sciences, University of Missouri, Columbia, MO S158A, USADepartment of Animal and Veterinary Sciences, University of Idaho, Caldwell, ID 1904 E, USAAnimal Sciences Research Center, Division of Animal Sciences, University of Missouri, Columbia, MO S158A, USADepartment of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 646310, USABackground: The objectives of this study were to identify loci, positional candidate genes, gene-sets, and pathways associated with spontaneous abortion (SA) in cattle and compare these results with previous human SA studies to determine if cattle are a good SA model for humans. Pregnancy was determined at gestation day 35 for Holstein heifers and cows. Genotypes from 43,984 SNPs of 499 pregnant heifers and 498 pregnant cows that calved at full term (FT) were compared to 62 heifers and 28 cows experiencing SA. A genome-wide association analysis, gene-set enrichment analysis−single nucleotide polymorphism, and ingenuity pathway analysis were used to identify regions, pathways, and master regulators associated with SA in heifers, cows, and a combined population. Results: Twenty-three loci and 21 positional candidate genes were associated (<i>p</i> < 1 × 10<sup>−5</sup>) with SA and one of these (KIR3DS1) has been associated with SA in humans. Eight gene-sets (NES > 3.0) were enriched in SA and one was previously reported as enriched in human SA. Four master regulators (<i>p</i> < 0.01) were associated with SA within two populations. Conclusions: One locus associated with SA was validated and 39 positional candidate and leading-edge genes and 2 gene-sets were enriched in SA in cattle and in humans.https://www.mdpi.com/2073-4425/10/12/954cattlespontaneous abortiondairyloci |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kayleen F. Oliver Alexandria M. Wahl Mataya Dick Jewel A. Toenges Jennifer N. Kiser Justine M. Galliou Joao G.N. Moraes Gregory W. Burns Joseph Dalton Thomas E. Spencer Holly L. Neibergs |
spellingShingle |
Kayleen F. Oliver Alexandria M. Wahl Mataya Dick Jewel A. Toenges Jennifer N. Kiser Justine M. Galliou Joao G.N. Moraes Gregory W. Burns Joseph Dalton Thomas E. Spencer Holly L. Neibergs Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows Genes cattle spontaneous abortion dairy loci |
author_facet |
Kayleen F. Oliver Alexandria M. Wahl Mataya Dick Jewel A. Toenges Jennifer N. Kiser Justine M. Galliou Joao G.N. Moraes Gregory W. Burns Joseph Dalton Thomas E. Spencer Holly L. Neibergs |
author_sort |
Kayleen F. Oliver |
title |
Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows |
title_short |
Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows |
title_full |
Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows |
title_fullStr |
Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows |
title_full_unstemmed |
Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows |
title_sort |
genomic analysis of spontaneous abortion in holstein heifers and primiparous cows |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2019-11-01 |
description |
Background: The objectives of this study were to identify loci, positional candidate genes, gene-sets, and pathways associated with spontaneous abortion (SA) in cattle and compare these results with previous human SA studies to determine if cattle are a good SA model for humans. Pregnancy was determined at gestation day 35 for Holstein heifers and cows. Genotypes from 43,984 SNPs of 499 pregnant heifers and 498 pregnant cows that calved at full term (FT) were compared to 62 heifers and 28 cows experiencing SA. A genome-wide association analysis, gene-set enrichment analysis−single nucleotide polymorphism, and ingenuity pathway analysis were used to identify regions, pathways, and master regulators associated with SA in heifers, cows, and a combined population. Results: Twenty-three loci and 21 positional candidate genes were associated (<i>p</i> < 1 × 10<sup>−5</sup>) with SA and one of these (KIR3DS1) has been associated with SA in humans. Eight gene-sets (NES > 3.0) were enriched in SA and one was previously reported as enriched in human SA. Four master regulators (<i>p</i> < 0.01) were associated with SA within two populations. Conclusions: One locus associated with SA was validated and 39 positional candidate and leading-edge genes and 2 gene-sets were enriched in SA in cattle and in humans. |
topic |
cattle spontaneous abortion dairy loci |
url |
https://www.mdpi.com/2073-4425/10/12/954 |
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