Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis
Glycyrrhiza uralensis is considered to be one of the most important herbs in traditional Chinese medicine due to its numerous pharmacological effects particularly its ability to relieve cough and act as a mucolytic. Based on previous research, these effects are mediated by a number of active ingredi...
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doaj-10400ce03d6049d68714c49d4a6bdd062020-11-24T21:36:02ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432013-12-013641642410.1016/j.apsb.2013.09.002Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensisHonghao ChenYing LiuXiaoqin ZhangXinjie ZhanChunsheng LiuGlycyrrhiza uralensis is considered to be one of the most important herbs in traditional Chinese medicine due to its numerous pharmacological effects particularly its ability to relieve cough and act as a mucolytic. Based on previous research, these effects are mediated by a number of active ingredients, especially glycyrrhizic acid (GA). In the present study, a gene encoding β-amyrin synthase (β-AS) involved in GA biosynthesis in G. uralensis has been cloned and expressed in Saccharomyces cerevisiae. The cloned enzyme showed similar activity to native enzymes isolated from other Glycyrrhiza species to catalyze the conversion of 2,3-oxidosqualene into β-amyrin. In fact the β-AS gene is particularly important in the GA biosynthetic pathway in G. uralensis. The complete sequence of the enzyme was determined and a phylogenetic tree based on the β-AS gene of G. uralensis and 20 other species was created. This showed that Glycyrrhiza glabra had the closest kinship with G. uralensis. The results of this work will be useful in determining how to improve the efficacy of G. uralensis by improving its GA content and in exploring the biosynthesis of GA in vitro.http://www.sciencedirect.com/science/article/pii/S2211383513000816β-Amyrin synthaseGlycyrrhiza uralensisGlycyrrhizic acidMolecular cloningHeterologous expressionGC–MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Honghao Chen Ying Liu Xiaoqin Zhang Xinjie Zhan Chunsheng Liu |
spellingShingle |
Honghao Chen Ying Liu Xiaoqin Zhang Xinjie Zhan Chunsheng Liu Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis Acta Pharmaceutica Sinica B β-Amyrin synthase Glycyrrhiza uralensis Glycyrrhizic acid Molecular cloning Heterologous expression GC–MS |
author_facet |
Honghao Chen Ying Liu Xiaoqin Zhang Xinjie Zhan Chunsheng Liu |
author_sort |
Honghao Chen |
title |
Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis |
title_short |
Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis |
title_full |
Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis |
title_fullStr |
Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis |
title_full_unstemmed |
Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis |
title_sort |
cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in glycyrrhiza uralensis |
publisher |
Elsevier |
series |
Acta Pharmaceutica Sinica B |
issn |
2211-3835 2211-3843 |
publishDate |
2013-12-01 |
description |
Glycyrrhiza uralensis is considered to be one of the most important herbs in traditional Chinese medicine due to its numerous pharmacological effects particularly its ability to relieve cough and act as a mucolytic. Based on previous research, these effects are mediated by a number of active ingredients, especially glycyrrhizic acid (GA). In the present study, a gene encoding β-amyrin synthase (β-AS) involved in GA biosynthesis in G. uralensis has been cloned and expressed in Saccharomyces cerevisiae. The cloned enzyme showed similar activity to native enzymes isolated from other Glycyrrhiza species to catalyze the conversion of 2,3-oxidosqualene into β-amyrin. In fact the β-AS gene is particularly important in the GA biosynthetic pathway in G. uralensis. The complete sequence of the enzyme was determined and a phylogenetic tree based on the β-AS gene of G. uralensis and 20 other species was created. This showed that Glycyrrhiza glabra had the closest kinship with G. uralensis. The results of this work will be useful in determining how to improve the efficacy of G. uralensis by improving its GA content and in exploring the biosynthesis of GA in vitro. |
topic |
β-Amyrin synthase Glycyrrhiza uralensis Glycyrrhizic acid Molecular cloning Heterologous expression GC–MS |
url |
http://www.sciencedirect.com/science/article/pii/S2211383513000816 |
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