Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model
Objective To investigate the application of 68Ga-PSMA-617 molecular probe for micro-PET imaging of gliomas tumor model. Methods DKFZ-PSMA-617, an effective prostate specific membrane antigen (PSMA) targeted probes, was chosen to be the precursor to be radiolabelled with 68Ga. The radio-chemical yiel...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | zho |
Published: |
Magazine House of Cancer Research on Prevention and Treatment
2018-07-01
|
Series: | Zhongliu Fangzhi Yanjiu |
Subjects: | |
Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0405.htm |
id |
doaj-1046e4e604c34f41bfc9076545c2b9b4 |
---|---|
record_format |
Article |
spelling |
doaj-1046e4e604c34f41bfc9076545c2b9b42020-11-25T02:40:47ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-07-0145744745210.3971/j.issn.1000-8578.2018.17.04058578.2018.17.0405Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas ModelHAN Xuedi0LIU Fei1GUO Xiaoyi2XU Xiaoxia3XIE Qinghua4LIU Teli5ZHU Hua6YANG Zhi7Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaObjective To investigate the application of 68Ga-PSMA-617 molecular probe for micro-PET imaging of gliomas tumor model. Methods DKFZ-PSMA-617, an effective prostate specific membrane antigen (PSMA) targeted probes, was chosen to be the precursor to be radiolabelled with 68Ga. The radio-chemical yield of 68Ga-PSMA-617 was analyzed by Radio-TLC. Cell uptake experiments were performed on PSMA (+) LNCaP, PSMA (-) PC-3 and U87MG cells. Nude mice bearing U87MG were injected with 68Ga-PSMA-617(7.4 MBq) via the tail vein. Micro-PET imaging was performed 5 and 40 min after the injection. Blocking studies were also performed in U87MG tumor-bearing mice after 40 min of co-injection of 68Ga-PSMA-617 with the PSMA inhibitor (S)-2-(3-((S)-1-carboxy-3-methylbutyl)ureido) pentanedioic acid (ZJ-43) (25 mg/kg). Results The radiotracer had a good radio-chemical yield of (99.0±1.9)% quickly tested by Radio-TLC. In cell uptake experiments, the specificity of 68Ga-PSMA-617 was confirmed in PSMA (+) LNCaP cells and PSMA (-) PC-3 cells; meanwhile, it proved the low expression level of PSMA in gliomas U87MG cell line. From micro-PET imaging, U87MG xenografts tumors were visualized and it showed good tumor-to-background value from 1.85±0.02 (5 min) to 3.62±0.175 (40 min) which could be blocked by ZJ-43. Conclusion 68Ga-PSMA-617 has the potential to be used not only in prostate cancer but also in gliomas tumor model.http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0405.htmprostate specific membrane antigenneo-vasculaturepetgliomagallium-68 |
collection |
DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
HAN Xuedi LIU Fei GUO Xiaoyi XU Xiaoxia XIE Qinghua LIU Teli ZHU Hua YANG Zhi |
spellingShingle |
HAN Xuedi LIU Fei GUO Xiaoyi XU Xiaoxia XIE Qinghua LIU Teli ZHU Hua YANG Zhi Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model Zhongliu Fangzhi Yanjiu prostate specific membrane antigen neo-vasculature pet glioma gallium-68 |
author_facet |
HAN Xuedi LIU Fei GUO Xiaoyi XU Xiaoxia XIE Qinghua LIU Teli ZHU Hua YANG Zhi |
author_sort |
HAN Xuedi |
title |
Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model |
title_short |
Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model |
title_full |
Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model |
title_fullStr |
Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model |
title_full_unstemmed |
Application of 68Ga-PSMA-617 Molecular Probe for Micro-PET Imaging of Gliomas Model |
title_sort |
application of 68ga-psma-617 molecular probe for micro-pet imaging of gliomas model |
publisher |
Magazine House of Cancer Research on Prevention and Treatment |
series |
Zhongliu Fangzhi Yanjiu |
issn |
1000-8578 1000-8578 |
publishDate |
2018-07-01 |
description |
Objective To investigate the application of 68Ga-PSMA-617 molecular probe for micro-PET imaging of gliomas tumor model. Methods DKFZ-PSMA-617, an effective prostate specific membrane antigen (PSMA) targeted probes, was chosen to be the precursor to be radiolabelled with 68Ga. The radio-chemical yield of 68Ga-PSMA-617 was analyzed by Radio-TLC. Cell uptake experiments were performed on PSMA (+) LNCaP, PSMA (-) PC-3 and U87MG cells. Nude mice bearing U87MG were injected with 68Ga-PSMA-617(7.4 MBq) via the tail vein. Micro-PET imaging was performed 5 and 40 min after the injection. Blocking studies were also performed in U87MG tumor-bearing mice after 40 min of co-injection of 68Ga-PSMA-617 with the PSMA inhibitor (S)-2-(3-((S)-1-carboxy-3-methylbutyl)ureido) pentanedioic acid (ZJ-43) (25 mg/kg). Results The radiotracer had a good radio-chemical yield of (99.0±1.9)% quickly tested by Radio-TLC. In cell uptake experiments, the specificity of 68Ga-PSMA-617 was confirmed in PSMA (+) LNCaP cells and PSMA (-) PC-3 cells; meanwhile, it proved the low expression level of PSMA in gliomas U87MG cell line. From micro-PET imaging, U87MG xenografts tumors were visualized and it showed good tumor-to-background value from 1.85±0.02 (5 min) to 3.62±0.175 (40 min) which could be blocked by ZJ-43. Conclusion 68Ga-PSMA-617 has the potential to be used not only in prostate cancer but also in gliomas tumor model. |
topic |
prostate specific membrane antigen neo-vasculature pet glioma gallium-68 |
url |
http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0405.htm |
work_keys_str_mv |
AT hanxuedi applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT liufei applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT guoxiaoyi applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT xuxiaoxia applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT xieqinghua applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT liuteli applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT zhuhua applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel AT yangzhi applicationof68gapsma617molecularprobeformicropetimagingofgliomasmodel |
_version_ |
1724779820808667136 |