Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury
Apoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury. Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury. With the notion tha...
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doaj-1052a7323b29465ebba43fc492b40e1f2020-11-24T23:28:47ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/827517827517Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic InjuryRoxana Rodríguez-Barrera0Ana M. Fernández-Presas1Elisa García2Adrian Flores-Romero3Susana Martiñón4Viridiana Yazmín González-Puertos5Humberto Mestre6Carmina Flores-Dominguez7Verónica Rodriguez-Mata8Mina Königsberg9Sandra Solano10Antonio Ibarra11Facultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, DF, CP 04510, MexicoFacultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoFacultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoFacultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, DF, CP 09340, MexicoFacultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoFacultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, DF, CP 04510, MexicoDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, DF, CP 09340, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, DF, CP 04510, MexicoFacultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, MexicoApoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury. Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury. With the notion that the aforementioned elements are apoptotic inducers, we hypothesized that INDPs would reduce apoptosis after SC injury. In order to test this assumption, adult rats were subjected to SC contusion and immunized either with A91 or phosphate buffered saline (PBS; control group). Seven days after injury, animals were euthanized to evaluate the number of apoptotic cells at the injury site. Apoptosis was evaluated using DAPI and TUNEL techniques; caspase-3 activity was also evaluated. To further elucidate the mechanisms through which A91 exerts this antiapoptotic effects we quantified tumor necrosis factor-alpha (TNF-α). To also demonstrate that the decrease in apoptotic cells correlated with a functional improvement, locomotor recovery was evaluated. Immunization with A91 significantly reduced the number of apoptotic cells and decreased caspase-3 activity and TNF-α concentration. Immunization with A91 also improved the functional recovery of injured rats. The present study shows the beneficial effect of INDPs on preventing apoptosis and provides more evidence on the neuroprotective mechanisms exerted by this strategy.http://dx.doi.org/10.1155/2013/827517 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roxana Rodríguez-Barrera Ana M. Fernández-Presas Elisa García Adrian Flores-Romero Susana Martiñón Viridiana Yazmín González-Puertos Humberto Mestre Carmina Flores-Dominguez Verónica Rodriguez-Mata Mina Königsberg Sandra Solano Antonio Ibarra |
spellingShingle |
Roxana Rodríguez-Barrera Ana M. Fernández-Presas Elisa García Adrian Flores-Romero Susana Martiñón Viridiana Yazmín González-Puertos Humberto Mestre Carmina Flores-Dominguez Verónica Rodriguez-Mata Mina Königsberg Sandra Solano Antonio Ibarra Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury BioMed Research International |
author_facet |
Roxana Rodríguez-Barrera Ana M. Fernández-Presas Elisa García Adrian Flores-Romero Susana Martiñón Viridiana Yazmín González-Puertos Humberto Mestre Carmina Flores-Dominguez Verónica Rodriguez-Mata Mina Königsberg Sandra Solano Antonio Ibarra |
author_sort |
Roxana Rodríguez-Barrera |
title |
Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury |
title_short |
Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury |
title_full |
Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury |
title_fullStr |
Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury |
title_full_unstemmed |
Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury |
title_sort |
immunization with a neural-derived peptide protects the spinal cord from apoptosis after traumatic injury |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Apoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury. Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury. With the notion that the aforementioned elements are apoptotic inducers, we hypothesized that INDPs would reduce apoptosis after SC injury. In order to test this assumption, adult rats were subjected to SC contusion and immunized either with A91 or phosphate buffered saline (PBS; control group). Seven days after injury, animals were euthanized to evaluate the number of apoptotic cells at the injury site. Apoptosis was evaluated using DAPI and TUNEL techniques; caspase-3 activity was also evaluated. To further elucidate the mechanisms through which A91 exerts this antiapoptotic effects we quantified tumor necrosis factor-alpha (TNF-α). To also demonstrate that the decrease in apoptotic cells correlated with a functional improvement, locomotor recovery was evaluated. Immunization with A91 significantly reduced the number of apoptotic cells and decreased caspase-3 activity and TNF-α concentration. Immunization with A91 also improved the functional recovery of injured rats. The present study shows the beneficial effect of INDPs on preventing apoptosis and provides more evidence on the neuroprotective mechanisms exerted by this strategy. |
url |
http://dx.doi.org/10.1155/2013/827517 |
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