Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran
Promoter polymorphism of cytokine genes may lead to inter-individual differences in cytokine levels, therefore, polymorphisms may associate with susceptibility to infectious diseases. In this study, we investigated a possible association between interleukin-10 (IL-10) -1082A⁄G (rs1800896) and inter...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2016-06-01
|
Series: | Iranian Journal of Allergy, Asthma and Immunology |
Subjects: | |
Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/655 |
id |
doaj-105cbee694aa401682c9c15ef6d71785 |
---|---|
record_format |
Article |
spelling |
doaj-105cbee694aa401682c9c15ef6d717852020-11-25T04:12:31ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492016-06-01153611Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of IranMohammad Asgharzadeh0Sajjad Ghorghanlu1Jalil Rashedi2Behroz Mahdavi Poor3Fatemeh Khaki-Khatibi4Seyyed Reza Moaddab5Hossein Samadi-Kafil6Mahya Pourostadi7Department of Laboratory Sciences, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, IranBiotechnology Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Laboratory Sciences, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Laboratory Sciences, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Infectious Disease and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Promoter polymorphism of cytokine genes may lead to inter-individual differences in cytokine levels, therefore, polymorphisms may associate with susceptibility to infectious diseases. In this study, we investigated a possible association between interleukin-10 (IL-10) -1082A⁄G (rs1800896) and interferon (IFN)-gamma +874T/A (rs2430561) promoter polymorphisms and tuberculosis (TB) in the Azeri population of Iran. IL-10 -1082G/A and IFN-gamma +874T/A single nucleotide polymorphisms (SNPs) were genotyped by amplification refractory mutation system (ARMS)-PCR in 200 healthy controls and 124 tuberculosis patients. IL-10 -1082 A allele was more frequent in the control group than in the patient group (p=0.001, odds ratio [OR]=2.183). On the other hand, the AA genotype was significantly more frequent in the control group (p=0.0001). The frequency of IFN-gamma +874 T allele was significantly higher in the controls (p=0.013, OR=1.56). There was no significant association between IFN-gamma +874 T/A genotypes and susceptibility to tuberculosis (p=0.078), but TT genotype was more frequent in the control group. Our findings suggest that interleukin-10 -1082G/A polymorphism may play an important role in susceptibility to tuberculosis in our population. On the other hand, the +874T allele, which has been suggested to be associated with high IFN-gamma levels, was significantly higher in the controls and TT genotype was also more frequent in the control group. Thus, +874 T allele may be associated with resistance to tuberculosis in this Azeri population of Iran. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/655AssociationInterferon-gammaInterleukin-10PolymorphismSusceptibilityTuberculosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Asgharzadeh Sajjad Ghorghanlu Jalil Rashedi Behroz Mahdavi Poor Fatemeh Khaki-Khatibi Seyyed Reza Moaddab Hossein Samadi-Kafil Mahya Pourostadi |
spellingShingle |
Mohammad Asgharzadeh Sajjad Ghorghanlu Jalil Rashedi Behroz Mahdavi Poor Fatemeh Khaki-Khatibi Seyyed Reza Moaddab Hossein Samadi-Kafil Mahya Pourostadi Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran Iranian Journal of Allergy, Asthma and Immunology Association Interferon-gamma Interleukin-10 Polymorphism Susceptibility Tuberculosis |
author_facet |
Mohammad Asgharzadeh Sajjad Ghorghanlu Jalil Rashedi Behroz Mahdavi Poor Fatemeh Khaki-Khatibi Seyyed Reza Moaddab Hossein Samadi-Kafil Mahya Pourostadi |
author_sort |
Mohammad Asgharzadeh |
title |
Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran |
title_short |
Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran |
title_full |
Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran |
title_fullStr |
Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran |
title_full_unstemmed |
Association of Promoter Polymorphisms of Interleukin-10 and Interferon-Gamma Genes with Tuberculosis in Azeri Population of Iran |
title_sort |
association of promoter polymorphisms of interleukin-10 and interferon-gamma genes with tuberculosis in azeri population of iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2016-06-01 |
description |
Promoter polymorphism of cytokine genes may lead to inter-individual differences in cytokine levels, therefore, polymorphisms may associate with susceptibility to infectious diseases. In this study, we investigated a possible association between interleukin-10 (IL-10) -1082A⁄G (rs1800896) and interferon (IFN)-gamma +874T/A (rs2430561) promoter polymorphisms and tuberculosis (TB) in the Azeri population of Iran. IL-10 -1082G/A and IFN-gamma +874T/A single nucleotide polymorphisms (SNPs) were genotyped by amplification refractory mutation system (ARMS)-PCR in 200 healthy controls and 124 tuberculosis patients. IL-10 -1082 A allele was more frequent in the control group than in the patient group (p=0.001, odds ratio [OR]=2.183). On the other hand, the AA genotype was significantly more frequent in the control group (p=0.0001). The frequency of IFN-gamma +874 T allele was significantly higher in the controls (p=0.013, OR=1.56). There was no significant association between IFN-gamma +874 T/A genotypes and susceptibility to tuberculosis (p=0.078), but TT genotype was more frequent in the control group. Our findings suggest that interleukin-10 -1082G/A polymorphism may play an important role in susceptibility to tuberculosis in our population. On the other hand, the +874T allele, which has been suggested to be associated with high IFN-gamma levels, was significantly higher in the controls and TT genotype was also more frequent in the control group. Thus, +874 T allele may be associated with resistance to tuberculosis in this Azeri population of Iran.
|
topic |
Association Interferon-gamma Interleukin-10 Polymorphism Susceptibility Tuberculosis |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/655 |
work_keys_str_mv |
AT mohammadasgharzadeh associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT sajjadghorghanlu associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT jalilrashedi associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT behrozmahdavipoor associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT fatemehkhakikhatibi associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT seyyedrezamoaddab associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT hosseinsamadikafil associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran AT mahyapourostadi associationofpromoterpolymorphismsofinterleukin10andinterferongammageneswithtuberculosisinazeripopulationofiran |
_version_ |
1724415258897940480 |