In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil
Objective: This study examined the in vivo differentiation of mesenchymal stem cells (MSCs) into insulin producing cells (IPCs) on electrospun poly-L-lactide acid (PLLA) scaffolds coated with Matricaria chammomila L. (chamomile) oil. Materials and Methods: In this interventional, experimental stu...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Royan Institute (ACECR), Tehran
2016-09-01
|
Series: | Cell Journal |
Subjects: | |
Online Access: | http://celljournal.org/web/journal/article/6089/download |
id |
doaj-1060e53646ef40779b2bc15d1142e03a |
---|---|
record_format |
Article |
spelling |
doaj-1060e53646ef40779b2bc15d1142e03a2020-11-25T00:41:49ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142016-09-01183310321In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. OilAfsaneh Fazili0Soghra Gholami1Bagher Minaie Zangi2Ehsan Seyedjafari3Mahdi Gholami4Department of Anatomy, School of Veterinary Medicine, University of Shiraz , Shiraz, IranDepartment of Anatomy, School of Veterinary Medicine, University of Shiraz , Shiraz, IranDepartment of Histology, Medical Sciences Faculty, Tehran University of Medical Sciences, Tehran, IranDepartment of Biotechnology, College of Science, University of Tehran, Tehran, IranFaculty of Pharmacy and Pharmaceutical Sciences, Research Center, Tehran University of Medical Sciences, Tehran, IranObjective: This study examined the in vivo differentiation of mesenchymal stem cells (MSCs) into insulin producing cells (IPCs) on electrospun poly-L-lactide acid (PLLA) scaffolds coated with Matricaria chammomila L. (chamomile) oil. Materials and Methods: In this interventional, experimental study adipose MSCs (AMSCs) were isolated from 12 adult male New Zealand white rabbits and characterized by flow cytometry. AMSCs were subsequently differentiated into osteogenic and adipogenic lines. Cells were seeded onto either a PLLA scaffold (control) or PLLA scaffold coated with chamomile oil (experimental). A total of 24 scaffolds were inserted into the pancreatic area of each rabbit and placement was confirmed by ultrasound. After 21 days, immunohistochemistry analysis of insulin-producing like cells on protein levels confirmed insulin expression of insulin producing cells (IPSCs). Real-time polymerase chain reaction (PCR) determined the expressions of genes related to pancreatic endocrine development and function. Results: Fourier transform infrared spectroscopy (FTIR) results confirmed the existence of oil on the surface of the PLLA scaffold. The results showed a new peak at 2854 cm-1 for the aliphatic CH2 bond. Pdx1 expression was 0.051 ± 0.007 in the experimental group and 0.009 ± 0.002 in the control group. There was significantly increased insulin expression in the scaffold coated with chamomile oil (0.09 ± 0.001) compared to control group (0.063 ± 0.009, P≤0.05). Both groups expressed Ngn3 and Pdx1 specific markers and pancreatic tissue was observed at 21 days post transplantation. Conclusion: The pancreatic region is an optimal site for differentiation of AMSCs to IPCs. Chamomile oil (as an antioxidant agent) can affect cell adhesion to the scaffold and increase cell differentiation. In addition, the oil may lead to increased blood glucose uptake in pathways in the muscles, liver and fatty tissue of a diabetic animal model by some probable molecular mechanisms.http://celljournal.org/web/journal/article/6089/downloadDifferentiationTransplantationScaffoldInsulin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Afsaneh Fazili Soghra Gholami Bagher Minaie Zangi Ehsan Seyedjafari Mahdi Gholami |
spellingShingle |
Afsaneh Fazili Soghra Gholami Bagher Minaie Zangi Ehsan Seyedjafari Mahdi Gholami In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil Cell Journal Differentiation Transplantation Scaffold Insulin |
author_facet |
Afsaneh Fazili Soghra Gholami Bagher Minaie Zangi Ehsan Seyedjafari Mahdi Gholami |
author_sort |
Afsaneh Fazili |
title |
In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil |
title_short |
In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil |
title_full |
In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil |
title_fullStr |
In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil |
title_full_unstemmed |
In Vivo Differentiation of Mesenchymal Stem Cells into Insulin Producing Cells on Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria chamomilla L. Oil |
title_sort |
in vivo differentiation of mesenchymal stem cells into insulin producing cells on electrospun poly-l-lactide acid scaffolds coated with matricaria chamomilla l. oil |
publisher |
Royan Institute (ACECR), Tehran |
series |
Cell Journal |
issn |
2228-5806 2228-5814 |
publishDate |
2016-09-01 |
description |
Objective: This study examined the in vivo differentiation of mesenchymal stem cells
(MSCs) into insulin producing cells (IPCs) on electrospun poly-L-lactide acid (PLLA) scaffolds
coated with Matricaria chammomila L. (chamomile) oil.
Materials and Methods: In this interventional, experimental study adipose MSCs
(AMSCs) were isolated from 12 adult male New Zealand white rabbits and characterized
by flow cytometry. AMSCs were subsequently differentiated into osteogenic
and adipogenic lines. Cells were seeded onto either a PLLA scaffold (control) or
PLLA scaffold coated with chamomile oil (experimental). A total of 24 scaffolds were
inserted into the pancreatic area of each rabbit and placement was confirmed by
ultrasound. After 21 days, immunohistochemistry analysis of insulin-producing like
cells on protein levels confirmed insulin expression of insulin producing cells (IPSCs).
Real-time polymerase chain reaction (PCR) determined the expressions of genes
related to pancreatic endocrine development and function.
Results: Fourier transform infrared spectroscopy (FTIR) results confirmed the existence
of oil on the surface of the PLLA scaffold. The results showed a new peak
at 2854 cm-1 for the aliphatic CH2 bond. Pdx1 expression was 0.051 ± 0.007 in the
experimental group and 0.009 ± 0.002 in the control group. There was significantly
increased insulin expression in the scaffold coated with chamomile oil (0.09 ± 0.001)
compared to control group (0.063 ± 0.009, P≤0.05). Both groups expressed Ngn3
and Pdx1 specific markers and pancreatic tissue was observed at 21 days post transplantation.
Conclusion: The pancreatic region is an optimal site for differentiation of AMSCs to IPCs.
Chamomile oil (as an antioxidant agent) can affect cell adhesion to the scaffold and increase
cell differentiation. In addition, the oil may lead to increased blood glucose uptake
in pathways in the muscles, liver and fatty tissue of a diabetic animal model by some probable
molecular mechanisms. |
topic |
Differentiation Transplantation Scaffold Insulin |
url |
http://celljournal.org/web/journal/article/6089/download |
work_keys_str_mv |
AT afsanehfazili invivodifferentiationofmesenchymalstemcellsintoinsulinproducingcellsonelectrospunpolyllactideacidscaffoldscoatedwithmatricariachamomillaloil AT soghragholami invivodifferentiationofmesenchymalstemcellsintoinsulinproducingcellsonelectrospunpolyllactideacidscaffoldscoatedwithmatricariachamomillaloil AT bagherminaiezangi invivodifferentiationofmesenchymalstemcellsintoinsulinproducingcellsonelectrospunpolyllactideacidscaffoldscoatedwithmatricariachamomillaloil AT ehsanseyedjafari invivodifferentiationofmesenchymalstemcellsintoinsulinproducingcellsonelectrospunpolyllactideacidscaffoldscoatedwithmatricariachamomillaloil AT mahdigholami invivodifferentiationofmesenchymalstemcellsintoinsulinproducingcellsonelectrospunpolyllactideacidscaffoldscoatedwithmatricariachamomillaloil |
_version_ |
1725285428277280768 |