Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children
Systemic inflammatory response syndrome (SIRS) is defined as the systemic host response to infection or a non-infectious factor. The purpose of this study was to evaluate the involvement of reactive oxygen species (ROS) in severe inflammation and to assess the discrimination strength of the neutroph...
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doaj-10642b6c613a412b94104cd6a0506b542021-02-24T00:03:47ZengMDPI AGInternational Journal of Environmental Research and Public Health1661-78271660-46012021-02-01182187218710.3390/ijerph18042187Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in ChildrenJanusz P. Sikora0Jarosław Sobczak1Dariusz Zawadzki2Przemysław Przewratil3Anna Wysocka4Monika Burzyńska5Department of Pediatric Emergency Medicine, 2nd Chair of Pediatrics, Central Clinical Hospital, Medical University of Łódź, 36/50 Sporna St., 91-738 Łódź, PolandDepartment of Pediatric Emergency Medicine, 2nd Chair of Pediatrics, Central Clinical Hospital, Medical University of Łódź, 36/50 Sporna St., 91-738 Łódź, PolandDepartment of Pediatric Emergency Medicine, 2nd Chair of Pediatrics, Central Clinical Hospital, Medical University of Łódź, 36/50 Sporna St., 91-738 Łódź, PolandDepartment of Pediatric Surgery and Oncology, Chair of Surgical Pediatrics, Central Clinical Hospital, Medical University of Łódź, 36/50 Sporna St., 91-738 Łódź, PolandDepartment of Pediatric Surgery and Oncology, Chair of Surgical Pediatrics, Central Clinical Hospital, Medical University of Łódź, 36/50 Sporna St., 91-738 Łódź, PolandDepartment of Epidemiology and Biostatistics, Chair of Social and Preventive Medicine, Medical University of Łódź, 7/9 Żeligowskiego St., 90-752 Łódź, PolandSystemic inflammatory response syndrome (SIRS) is defined as the systemic host response to infection or a non-infectious factor. The purpose of this study was to evaluate the involvement of reactive oxygen species (ROS) in severe inflammation and to assess the discrimination strength of the neutrophil BURSTTEST assay regarding its etiology in three groups of patients (sepsis, burns, and bone fractures) who met the SIRS criteria. The neutrophil activation (respiratory burst of granulocytes as well as p55 and p75 tumor necrosis factor (TNF-α) receptor expression) was evaluated twice using flow cytometry, and the results were compared with healthy controls and among SIRS subjects. A decreased oxygen metabolism in neutrophils after <i>E.</i> <i>coli</i> stimulation and increased TNF-α receptor expression were found in septic and burned patients on admission, while ROS production augmented and TNF-α receptor expression diminished with the applied therapy. The significant differences in neutrophil respiratory burst intensity among septic and burned patients and those with sepsis and bone fractures were found (however, there were not any such differences between patients with thermal and mechanical injuries). This study indicates that the neutrophil BURSTTEST evaluation might be a clinically reliable marker for differentiating the SIRS etiology.https://www.mdpi.com/1660-4601/18/4/2187neutrophilsrespiratory burstSIRSROSTNF-α receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Janusz P. Sikora Jarosław Sobczak Dariusz Zawadzki Przemysław Przewratil Anna Wysocka Monika Burzyńska |
spellingShingle |
Janusz P. Sikora Jarosław Sobczak Dariusz Zawadzki Przemysław Przewratil Anna Wysocka Monika Burzyńska Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children International Journal of Environmental Research and Public Health neutrophils respiratory burst SIRS ROS TNF-α receptor |
author_facet |
Janusz P. Sikora Jarosław Sobczak Dariusz Zawadzki Przemysław Przewratil Anna Wysocka Monika Burzyńska |
author_sort |
Janusz P. Sikora |
title |
Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children |
title_short |
Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children |
title_full |
Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children |
title_fullStr |
Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children |
title_full_unstemmed |
Respiratory Burst and TNF-α Receptor Expression of Neutrophils after Sepsis and Severe Injury-Induced Inflammation in Children |
title_sort |
respiratory burst and tnf-α receptor expression of neutrophils after sepsis and severe injury-induced inflammation in children |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1661-7827 1660-4601 |
publishDate |
2021-02-01 |
description |
Systemic inflammatory response syndrome (SIRS) is defined as the systemic host response to infection or a non-infectious factor. The purpose of this study was to evaluate the involvement of reactive oxygen species (ROS) in severe inflammation and to assess the discrimination strength of the neutrophil BURSTTEST assay regarding its etiology in three groups of patients (sepsis, burns, and bone fractures) who met the SIRS criteria. The neutrophil activation (respiratory burst of granulocytes as well as p55 and p75 tumor necrosis factor (TNF-α) receptor expression) was evaluated twice using flow cytometry, and the results were compared with healthy controls and among SIRS subjects. A decreased oxygen metabolism in neutrophils after <i>E.</i> <i>coli</i> stimulation and increased TNF-α receptor expression were found in septic and burned patients on admission, while ROS production augmented and TNF-α receptor expression diminished with the applied therapy. The significant differences in neutrophil respiratory burst intensity among septic and burned patients and those with sepsis and bone fractures were found (however, there were not any such differences between patients with thermal and mechanical injuries). This study indicates that the neutrophil BURSTTEST evaluation might be a clinically reliable marker for differentiating the SIRS etiology. |
topic |
neutrophils respiratory burst SIRS ROS TNF-α receptor |
url |
https://www.mdpi.com/1660-4601/18/4/2187 |
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