Protists Within Corals: The Hidden Diversity
Previous observations suggested that microbial communities contribute to coral health and the ecological resilience of coral reefs. However, most studies of coral microbiology focused on prokaryotes and the endosymbiotic algae Symbiodinium. In contrast, knowledge concerning diversity of other protis...
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doaj-1066e04a6f724513b3e3ab5b6dbca70e2020-11-24T22:07:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-08-01910.3389/fmicb.2018.02043407707Protists Within Corals: The Hidden DiversityCamille Clerissi0Sébastien Brunet1Jeremie Vidal-Dupiol2Mehdi Adjeroud3Pierre Lepage4Laure Guillou5Jean-Michel Escoubas6Eve Toulza7Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, FranceMcGill University and Génome Québec Innovation Centre, Montréal, QC, CanadaIFREMER, IHPE UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, Montpellier, FranceInstitut de Recherche pour le Développement, UMR 9220 ENTROPIE & Laboratoire d’Excellence CORAIL, Université de Perpignan, Perpignan, FranceMcGill University and Génome Québec Innovation Centre, Montréal, QC, CanadaCNRS, UMR 7144, Sorbonne Universités, Université Pierre et Marie Curie – Paris 6, Station Biologique de Roscoff, Roscoff, FranceCNRS, IHPE UMR 5244, Univ. Perpignan Via Domitia, IFREMER, Univ. Montpellier, Montpellier, FranceUniv. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, FrancePrevious observations suggested that microbial communities contribute to coral health and the ecological resilience of coral reefs. However, most studies of coral microbiology focused on prokaryotes and the endosymbiotic algae Symbiodinium. In contrast, knowledge concerning diversity of other protists is still lacking, possibly due to methodological constraints. As most eukaryotic DNA in coral samples was derived from hosts, protist diversity was missed in metagenome analyses. To tackle this issue, we designed blocking primers for Scleractinia sequences amplified with two primer sets that targeted variable loops of the 18S rRNA gene (18SV1V2 and 18SV4). These blocking primers were used on environmental colonies of Pocillopora damicornis sensu lato from two regions with contrasting thermal regimes (Djibouti and New Caledonia). In addition to Symbiodinium clades A/C/D, Licnophora and unidentified coccidia genera were found in many samples. In particular, coccidian sequences formed a robust monophyletic clade with other protists identified in Agaricia, Favia, Montastraea, Mycetophyllia, Porites, and Siderastrea coral colonies. Moreover, Licnophora and coccidians had different distributions between the two geographic regions. A similar pattern was observed between Symbiodinium clades C and A/D. Although we were unable to identify factors responsible for this pattern, nor were we able to confirm that these taxa were closely associated with corals, we believe that these primer sets and the associated blocking primers offer new possibilities to describe the hidden diversity of protists within different coral species.https://www.frontiersin.org/article/10.3389/fmicb.2018.02043/fullholobiontprotistssymbiosismetabarcodingblocking primerScleractinia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Camille Clerissi Sébastien Brunet Jeremie Vidal-Dupiol Mehdi Adjeroud Pierre Lepage Laure Guillou Jean-Michel Escoubas Eve Toulza |
spellingShingle |
Camille Clerissi Sébastien Brunet Jeremie Vidal-Dupiol Mehdi Adjeroud Pierre Lepage Laure Guillou Jean-Michel Escoubas Eve Toulza Protists Within Corals: The Hidden Diversity Frontiers in Microbiology holobiont protists symbiosis metabarcoding blocking primer Scleractinia |
author_facet |
Camille Clerissi Sébastien Brunet Jeremie Vidal-Dupiol Mehdi Adjeroud Pierre Lepage Laure Guillou Jean-Michel Escoubas Eve Toulza |
author_sort |
Camille Clerissi |
title |
Protists Within Corals: The Hidden Diversity |
title_short |
Protists Within Corals: The Hidden Diversity |
title_full |
Protists Within Corals: The Hidden Diversity |
title_fullStr |
Protists Within Corals: The Hidden Diversity |
title_full_unstemmed |
Protists Within Corals: The Hidden Diversity |
title_sort |
protists within corals: the hidden diversity |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-08-01 |
description |
Previous observations suggested that microbial communities contribute to coral health and the ecological resilience of coral reefs. However, most studies of coral microbiology focused on prokaryotes and the endosymbiotic algae Symbiodinium. In contrast, knowledge concerning diversity of other protists is still lacking, possibly due to methodological constraints. As most eukaryotic DNA in coral samples was derived from hosts, protist diversity was missed in metagenome analyses. To tackle this issue, we designed blocking primers for Scleractinia sequences amplified with two primer sets that targeted variable loops of the 18S rRNA gene (18SV1V2 and 18SV4). These blocking primers were used on environmental colonies of Pocillopora damicornis sensu lato from two regions with contrasting thermal regimes (Djibouti and New Caledonia). In addition to Symbiodinium clades A/C/D, Licnophora and unidentified coccidia genera were found in many samples. In particular, coccidian sequences formed a robust monophyletic clade with other protists identified in Agaricia, Favia, Montastraea, Mycetophyllia, Porites, and Siderastrea coral colonies. Moreover, Licnophora and coccidians had different distributions between the two geographic regions. A similar pattern was observed between Symbiodinium clades C and A/D. Although we were unable to identify factors responsible for this pattern, nor were we able to confirm that these taxa were closely associated with corals, we believe that these primer sets and the associated blocking primers offer new possibilities to describe the hidden diversity of protists within different coral species. |
topic |
holobiont protists symbiosis metabarcoding blocking primer Scleractinia |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.02043/full |
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