A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer...
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2012-01-01
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doaj-1083ce7beaf045249e18e7e9a0328fca2021-03-04T00:27:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4248510.1371/journal.pone.0042485A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.Hideyuki TerazonoHyonchol KimMasahito HayashiAkihiro HattoriFumimasa NomuraTomoyuki KanekoKenji YasudaA non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870332/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hideyuki Terazono Hyonchol Kim Masahito Hayashi Akihiro Hattori Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda |
spellingShingle |
Hideyuki Terazono Hyonchol Kim Masahito Hayashi Akihiro Hattori Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. PLoS ONE |
author_facet |
Hideyuki Terazono Hyonchol Kim Masahito Hayashi Akihiro Hattori Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda |
author_sort |
Hideyuki Terazono |
title |
A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. |
title_short |
A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. |
title_full |
A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. |
title_fullStr |
A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. |
title_full_unstemmed |
A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. |
title_sort |
non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870332/?tool=EBI |
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