Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802)
The aim of this work was to study the testicular morphometry of captivity-bred adult bullfrogs. Fifteen young adult male were studied, in the rainy season and a lengthy photoperiod. The GSI was established at 0.15%. The nuclear diameter of germinative and Leydig cells, the nucleolus diameter of Sert...
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Instituto de Tecnologia do Paraná (Tecpar)
2009-12-01
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doaj-10b46ccaf347433f83b154b61796968a2020-11-24T22:38:23ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242009-12-015261461147210.1590/S1516-89132009000600018Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802)Jaqueline CarlosSérgio Luis Pinto da MattaThe aim of this work was to study the testicular morphometry of captivity-bred adult bullfrogs. Fifteen young adult male were studied, in the rainy season and a lengthy photoperiod. The GSI was established at 0.15%. The nuclear diameter of germinative and Leydig cells, the nucleolus diameter of Sertoli cells and the area of cysts and tubules were determined and the mean number of ISPC, IISPC and SPT per cyst and the mean number of cysts per tubule was estimated. The nucleoplasmatic proportion of the nucleus of the Leydig cell was 76.22%, indicating less cytoplasmic activity. Eight generations of spermatogonia were found. The spermatogenesis efficiency in meiosis and in mitosis was 63 and 49%, respectively. The spermatogenesis of bullfrog fited in the pattern of other captivity Anurans, with differences as the morphology of Sertoli and Leydig cells nuclei.<br>A morfometria é uma importante ferramenta para a biologia estrutural, permitindo estudos estereológicos e análises quantitativas. Existem muitos pontos a serem esclarecidos sobre a morfometria testicular desta espécie, que objetivamos desvendar neste trabalho. Quinze machos adultos foram estudados, em período chuvoso e de fotoperíodo longo (dezembro, 2000). O IGS encontrado foi de 0.15%. O diâmetro nuclear das células germinativas e da célula de Leydig, o diâmetro nucleolar das células de Sertoli e a área dos cistos e túbulos foram determinados. O número médio de ISPC, IISPC e SPT por cisto e o número médio de cisto por túbulo foi estimado. A proporção nucleoplasmática do núcleo da célula de Leydig foi de 76.22%, indicando pouca atividade citoplasmática. Oito gerações de espermatogônia foram estimadas. A eficiência da espermatogênese na meiose e mitose foi de 63% e49%, respectivamente. A espermatogênese de rãtouro segue os padrões dos demais Anuros de cativeiro, apresentando diferenças nos núcleos das c��lulas de Sertoli e Leydig.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600018spermatogenesisSertoliLeydigGSI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jaqueline Carlos Sérgio Luis Pinto da Matta |
spellingShingle |
Jaqueline Carlos Sérgio Luis Pinto da Matta Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802) Brazilian Archives of Biology and Technology spermatogenesis Sertoli Leydig GSI |
author_facet |
Jaqueline Carlos Sérgio Luis Pinto da Matta |
author_sort |
Jaqueline Carlos |
title |
Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802) |
title_short |
Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802) |
title_full |
Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802) |
title_fullStr |
Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802) |
title_full_unstemmed |
Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802) |
title_sort |
microscopic morphology and testis morphometry of captivity-bred adult bullfrogs (lithobates catesbeianus shaw, 1802) |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1516-8913 1678-4324 |
publishDate |
2009-12-01 |
description |
The aim of this work was to study the testicular morphometry of captivity-bred adult bullfrogs. Fifteen young adult male were studied, in the rainy season and a lengthy photoperiod. The GSI was established at 0.15%. The nuclear diameter of germinative and Leydig cells, the nucleolus diameter of Sertoli cells and the area of cysts and tubules were determined and the mean number of ISPC, IISPC and SPT per cyst and the mean number of cysts per tubule was estimated. The nucleoplasmatic proportion of the nucleus of the Leydig cell was 76.22%, indicating less cytoplasmic activity. Eight generations of spermatogonia were found. The spermatogenesis efficiency in meiosis and in mitosis was 63 and 49%, respectively. The spermatogenesis of bullfrog fited in the pattern of other captivity Anurans, with differences as the morphology of Sertoli and Leydig cells nuclei.<br>A morfometria é uma importante ferramenta para a biologia estrutural, permitindo estudos estereológicos e análises quantitativas. Existem muitos pontos a serem esclarecidos sobre a morfometria testicular desta espécie, que objetivamos desvendar neste trabalho. Quinze machos adultos foram estudados, em período chuvoso e de fotoperíodo longo (dezembro, 2000). O IGS encontrado foi de 0.15%. O diâmetro nuclear das células germinativas e da célula de Leydig, o diâmetro nucleolar das células de Sertoli e a área dos cistos e túbulos foram determinados. O número médio de ISPC, IISPC e SPT por cisto e o número médio de cisto por túbulo foi estimado. A proporção nucleoplasmática do núcleo da célula de Leydig foi de 76.22%, indicando pouca atividade citoplasmática. Oito gerações de espermatogônia foram estimadas. A eficiência da espermatogênese na meiose e mitose foi de 63% e49%, respectivamente. A espermatogênese de rãtouro segue os padrões dos demais Anuros de cativeiro, apresentando diferenças nos núcleos das c��lulas de Sertoli e Leydig. |
topic |
spermatogenesis Sertoli Leydig GSI |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600018 |
work_keys_str_mv |
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