Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes
Chondrocyte preparation with the safety and efficiency is the first step in cartilage regenerative medicine. To prepare a chondrocyte proliferation medium that does not contain fetal bovine serum (FBS) and that provides more than a 1000-fold increase in cell numbers within approximately 1 month, we...
Main Authors: | , , , , , , , , , , |
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Format: | Article |
Language: | English |
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SAGE Publishing
2005-10-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.3727/000000005783982675 |
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doaj-10d06afd59244d80a4aa35b765c7f79c |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tsuguharu Takahashi Toru Ogasawara Junji Kishimoto Guangyao Liu Hirotaka Asato Takashi Nakatsuka Eijyu Uchinuma Kozo Nakamura Hiroshi Kawaguchi Tsuyoshi Takato Kazuto Hoshi M.D., Ph.D. |
spellingShingle |
Tsuguharu Takahashi Toru Ogasawara Junji Kishimoto Guangyao Liu Hirotaka Asato Takashi Nakatsuka Eijyu Uchinuma Kozo Nakamura Hiroshi Kawaguchi Tsuyoshi Takato Kazuto Hoshi M.D., Ph.D. Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes Cell Transplantation |
author_facet |
Tsuguharu Takahashi Toru Ogasawara Junji Kishimoto Guangyao Liu Hirotaka Asato Takashi Nakatsuka Eijyu Uchinuma Kozo Nakamura Hiroshi Kawaguchi Tsuyoshi Takato Kazuto Hoshi M.D., Ph.D. |
author_sort |
Tsuguharu Takahashi |
title |
Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes |
title_short |
Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes |
title_full |
Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes |
title_fullStr |
Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes |
title_full_unstemmed |
Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes |
title_sort |
synergistic effects of fgf-2 with insulin or igf-i on the proliferation of human auricular chondrocytes |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2005-10-01 |
description |
Chondrocyte preparation with the safety and efficiency is the first step in cartilage regenerative medicine. To prepare a chondrocyte proliferation medium that does not contain fetal bovine serum (FBS) and that provides more than a 1000-fold increase in cell numbers within approximately 1 month, we attempted to use the medium containing 5% human serum (HS), but it exerted no more than twofold increase in 2 weeks. To compensate for the limited proliferation ability in HS, we investigated the combinational effects of 12 factors [i.e., fibroblast growth factor(FGF)-2, insulin-like growth factor(IGF)-I, insulin, bone morphogenetic protein-2, parathyroid hormone, growth hormone, dexamethasone, 1α25-dihydroxy vitamin D 3 , L-3,3′,5′-triodothyronine, interleukine-1 receptor antagonist, 17β-estradiol, and testosterone] on the proliferation of human auricular chondrocytes by analysis of variance in fractional factorial design. As a result, FGF-2, dexamethasone, insulin, and IGF-I possessed promotional effects on proliferation, while the combination of FGF-2 with insulin or IGF-I synergistically enhanced the proliferation. Actually, the chondrocytes increased 7.5-fold in number in 2 weeks in a medium containing 5% HS with 10 ng/ml FGF-2, while the cell number synergistically gained a 10–12-fold increase with 5 μg/ml insulin or 100 ng/ml IGF-I in the same period. The proliferation effects were more enhanced at a concentration of 100 ng/ml for FGF-2, and especially for the combination of 100 ng/ml FGF-2 and 5 μg/ml insulin (approximately 16-fold within 2 weeks). In the long-term culture with repeated passaging, this combination provided more than 10,000-fold within 8 weeks (i.e., passage 4). Thus, we concluded that such a combination of FGF-2 with insulin or IGF-I may be useful for promotion of auricular chondrocyte proliferation in a clinical application for cartilage regeneration. |
url |
https://doi.org/10.3727/000000005783982675 |
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doaj-10d06afd59244d80a4aa35b765c7f79c2020-11-25T03:45:17ZengSAGE PublishingCell Transplantation0963-68971555-38922005-10-011410.3727/000000005783982675Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular ChondrocytesTsuguharu Takahashi0Toru Ogasawara1Junji Kishimoto2Guangyao Liu3Hirotaka Asato4Takashi Nakatsuka5Eijyu Uchinuma6Kozo Nakamura7Hiroshi Kawaguchi8Tsuyoshi Takato9Kazuto Hoshi M.D., Ph.D.10Division of Tissue Engineering, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Oral & Maxillofacial Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Clinical Bioinformatics, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Orthopaedics Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Plastic & Reconstructive Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Plastic & Reconstructive Surgery, Saitama Medical School, Kerohongo 38, keroyama-cho, Iruma, Saitama 350–0495, JapanDepartment of Plastic & Reconstructive Surgery, Kitasato University, Kitasato 1–15–1, Sagamihara, Kanagawa 228–8555, JapanDepartment of Clinical Bioinformatics, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Clinical Bioinformatics, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDepartment of Oral & Maxillofacial Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanDivision of Tissue Engineering, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Hongo 7–3–1, Bunkyo-Ku, Tokyo 113–0033, JapanChondrocyte preparation with the safety and efficiency is the first step in cartilage regenerative medicine. To prepare a chondrocyte proliferation medium that does not contain fetal bovine serum (FBS) and that provides more than a 1000-fold increase in cell numbers within approximately 1 month, we attempted to use the medium containing 5% human serum (HS), but it exerted no more than twofold increase in 2 weeks. To compensate for the limited proliferation ability in HS, we investigated the combinational effects of 12 factors [i.e., fibroblast growth factor(FGF)-2, insulin-like growth factor(IGF)-I, insulin, bone morphogenetic protein-2, parathyroid hormone, growth hormone, dexamethasone, 1α25-dihydroxy vitamin D 3 , L-3,3′,5′-triodothyronine, interleukine-1 receptor antagonist, 17β-estradiol, and testosterone] on the proliferation of human auricular chondrocytes by analysis of variance in fractional factorial design. As a result, FGF-2, dexamethasone, insulin, and IGF-I possessed promotional effects on proliferation, while the combination of FGF-2 with insulin or IGF-I synergistically enhanced the proliferation. Actually, the chondrocytes increased 7.5-fold in number in 2 weeks in a medium containing 5% HS with 10 ng/ml FGF-2, while the cell number synergistically gained a 10–12-fold increase with 5 μg/ml insulin or 100 ng/ml IGF-I in the same period. The proliferation effects were more enhanced at a concentration of 100 ng/ml for FGF-2, and especially for the combination of 100 ng/ml FGF-2 and 5 μg/ml insulin (approximately 16-fold within 2 weeks). In the long-term culture with repeated passaging, this combination provided more than 10,000-fold within 8 weeks (i.e., passage 4). Thus, we concluded that such a combination of FGF-2 with insulin or IGF-I may be useful for promotion of auricular chondrocyte proliferation in a clinical application for cartilage regeneration.https://doi.org/10.3727/000000005783982675 |