COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21
Barbara Kuźnar-Kamińska,1 Justyna Mikuła-Pietrasik,2 Patrycja Sosińska,2 Krzysztof Książek,2 Halina Batura-Gabryel1 1Department of Pulmonology, Allergology and Respiratory Oncology, 2Department of Pathophysiology, Poznań University of Medical Sciences, Poznań, Poland Abstract: Patients wi...
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doaj-10e27af787b04a5d86463892e936135a2020-11-24T23:39:37ZengDove Medical PressInternational Journal of COPD1178-20052016-05-012016Issue 11061106627100COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21Kuźnar-Kamińska BMikuła-Pietrasik JSosińska PKsiążek KBatura-Gabryel HBarbara Kuźnar-Kamińska,1 Justyna Mikuła-Pietrasik,2 Patrycja Sosińska,2 Krzysztof Książek,2 Halina Batura-Gabryel1 1Department of Pulmonology, Allergology and Respiratory Oncology, 2Department of Pathophysiology, Poznań University of Medical Sciences, Poznań, Poland Abstract: Patients with COPD develop lung cancer more frequently than healthy smokers. At the same time, molecular mediators promoting various aspects of cancer cell progression are still elusive. In this report, we examined whether COPD can be coupled with increased migration of non-small-cell lung cancer cells A549 and, if so, whether this effect may be related to altered production and activity of chemokines CCL21, CXCL5, and CXCL12. The study showed that the migration of A549 cells through the polycarbonate membrane and basement membrane extract toward a chemotactic gradient elicited by serum from patients with COPD was markedly higher as compared with serum from healthy donors. The concentration of CCL21 and CXCL12, but not CXCL5, in serum from patients with COPD was also increased. Experiments in which CCL21- and CXCL12-dependent signaling was blocked revealed that increased migration of the cancer cells upon treatment with serum from patients with COPD was mediated exclusively by CCL21. Collectively, our results indicate that COPD may contribute to the progression of lung cancer via CCL21-dependent intensification of cancer cell migration. Keywords: chemokines, COPD, lung cancer, migrationhttps://www.dovepress.com/copd-promotes-migration-of-a549-lung-cancer-cells-the-role-of-chemokin-peer-reviewed-article-COPDchemokinesCOPDlung cancermigration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kuźnar-Kamińska B Mikuła-Pietrasik J Sosińska P Książek K Batura-Gabryel H |
spellingShingle |
Kuźnar-Kamińska B Mikuła-Pietrasik J Sosińska P Książek K Batura-Gabryel H COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21 International Journal of COPD chemokines COPD lung cancer migration |
author_facet |
Kuźnar-Kamińska B Mikuła-Pietrasik J Sosińska P Książek K Batura-Gabryel H |
author_sort |
Kuźnar-Kamińska B |
title |
COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21 |
title_short |
COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21 |
title_full |
COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21 |
title_fullStr |
COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21 |
title_full_unstemmed |
COPD promotes migration of A549 lung cancer cells: the role of chemokine CCL21 |
title_sort |
copd promotes migration of a549 lung cancer cells: the role of chemokine ccl21 |
publisher |
Dove Medical Press |
series |
International Journal of COPD |
issn |
1178-2005 |
publishDate |
2016-05-01 |
description |
Barbara Kuźnar-Kamińska,1 Justyna Mikuła-Pietrasik,2 Patrycja Sosińska,2 Krzysztof Książek,2 Halina Batura-Gabryel1 1Department of Pulmonology, Allergology and Respiratory Oncology, 2Department of Pathophysiology, Poznań University of Medical Sciences, Poznań, Poland Abstract: Patients with COPD develop lung cancer more frequently than healthy smokers. At the same time, molecular mediators promoting various aspects of cancer cell progression are still elusive. In this report, we examined whether COPD can be coupled with increased migration of non-small-cell lung cancer cells A549 and, if so, whether this effect may be related to altered production and activity of chemokines CCL21, CXCL5, and CXCL12. The study showed that the migration of A549 cells through the polycarbonate membrane and basement membrane extract toward a chemotactic gradient elicited by serum from patients with COPD was markedly higher as compared with serum from healthy donors. The concentration of CCL21 and CXCL12, but not CXCL5, in serum from patients with COPD was also increased. Experiments in which CCL21- and CXCL12-dependent signaling was blocked revealed that increased migration of the cancer cells upon treatment with serum from patients with COPD was mediated exclusively by CCL21. Collectively, our results indicate that COPD may contribute to the progression of lung cancer via CCL21-dependent intensification of cancer cell migration. Keywords: chemokines, COPD, lung cancer, migration |
topic |
chemokines COPD lung cancer migration |
url |
https://www.dovepress.com/copd-promotes-migration-of-a549-lung-cancer-cells-the-role-of-chemokin-peer-reviewed-article-COPD |
work_keys_str_mv |
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