Development of Microsatellite Markers for <i>Lagerstroemia indica</i> (Lythraceae) and Related Species

• Main Authors: Yang Liu, Dan He, Ming Cai, Wan Tang, Xiao-Yuan Li, Hui-Tang Pan, Qi-Xiang Zhang
• Format: Article
• Language: English
• Published: Wiley 2013-02-01
• Series: Applications in Plant Sciences
• Subjects: cross-transferrable; Lagerstroemia indica; Lythraceae; microsatellite; SSR-enriched library
• Online Access: http://www.bioone.org/doi/full/10.3732/apps.1200203
• ISSN: 2168-0450

Premise of the study: Microsatellite markers were developed and characterized to analyze genetic diversity within <i>Lagerstroemia</i> cultivars and related species. Methods and Results: Using simple sequence repeat (SSR)-enriched libraries, 11 species-specific polymorphic genomic SSRs were developed from <i>L. indica</i> ‘Hong Die Fei Wu’. All primers were tested on 48 L. indica individuals from China, the United States, and France. The primers amplified four to 12 alleles per locus, including di-, tri-, and tetranucleotide repeats. Observed and expected heterozygosities ranged from 0.1875 to 0.7609 and 0.2836 to 0.8385, respectively. The primers were also highly cross-transferrable to <i>L. subcostata</i>, <i>L. limii</i>, <i>L. fauriei</i>, <i>L. caudata</i>, and <i>L. speciosa</i>. Conclusions: The new primers will enlarge the bank of SSRs available to genetic research of <i>Lagerstroemia</i>. These SSR markers will facilitate population genetics and molecular marker-assisted selection of <i>L. indica</i>.