Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids
A fraction of commercially available lyophilized concanavalin A (ConA) shows low mannose binding activity. In this study, we investigated the effects of the component ion and water content of hydrated ionic liquids (ILs) as treatment solvent on the mannose binding ability of lyophilized ConA. While...
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doaj-10ebd38116dd47999d676cc5800d845d2020-12-24T00:05:45ZengMDPI AGApplied Sciences2076-34172021-12-0111575710.3390/app11010057Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic LiquidsKyoko Fujita0Ryota Fujii1Kimiyoshi Ichida2Department of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, JapanDepartment of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, JapanDepartment of Pathophysiology, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, JapanA fraction of commercially available lyophilized concanavalin A (ConA) shows low mannose binding activity. In this study, we investigated the effects of the component ion and water content of hydrated ionic liquids (ILs) as treatment solvent on the mannose binding ability of lyophilized ConA. While high mannose binding ability was observed treated in hydrated ILs composed of dihydrogen phosphate anion with three water molecules per ion pair, it decreased with increase in water content. In contrast, no increase in mannose binding ability was observed after treatment with hydrated ILs composed of bromide anions. A steady response in mannose binding was observed after treatment with hydrated cholinium dihydrogen phosphate ([ch][dhp]). Following treatment with hydrated [ch][dhp] with three water molecules per ion pair, the mannose binding ability of lyophilized ConA increased twofold, while the low and high mannose binding ConA fraction showed an increase of 3.3 and 1.8 in the binding response, respectively.https://www.mdpi.com/2076-3417/11/1/57hydrated ionic liquidsrenaturationwater contentmannose binding ability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyoko Fujita Ryota Fujii Kimiyoshi Ichida |
spellingShingle |
Kyoko Fujita Ryota Fujii Kimiyoshi Ichida Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids Applied Sciences hydrated ionic liquids renaturation water content mannose binding ability |
author_facet |
Kyoko Fujita Ryota Fujii Kimiyoshi Ichida |
author_sort |
Kyoko Fujita |
title |
Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids |
title_short |
Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids |
title_full |
Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids |
title_fullStr |
Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids |
title_full_unstemmed |
Renaturation of Lyophilized Concanavalin a Treated in Water Content Controlled Hydrated Ionic Liquids |
title_sort |
renaturation of lyophilized concanavalin a treated in water content controlled hydrated ionic liquids |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-12-01 |
description |
A fraction of commercially available lyophilized concanavalin A (ConA) shows low mannose binding activity. In this study, we investigated the effects of the component ion and water content of hydrated ionic liquids (ILs) as treatment solvent on the mannose binding ability of lyophilized ConA. While high mannose binding ability was observed treated in hydrated ILs composed of dihydrogen phosphate anion with three water molecules per ion pair, it decreased with increase in water content. In contrast, no increase in mannose binding ability was observed after treatment with hydrated ILs composed of bromide anions. A steady response in mannose binding was observed after treatment with hydrated cholinium dihydrogen phosphate ([ch][dhp]). Following treatment with hydrated [ch][dhp] with three water molecules per ion pair, the mannose binding ability of lyophilized ConA increased twofold, while the low and high mannose binding ConA fraction showed an increase of 3.3 and 1.8 in the binding response, respectively. |
topic |
hydrated ionic liquids renaturation water content mannose binding ability |
url |
https://www.mdpi.com/2076-3417/11/1/57 |
work_keys_str_mv |
AT kyokofujita renaturationoflyophilizedconcanavalinatreatedinwatercontentcontrolledhydratedionicliquids AT ryotafujii renaturationoflyophilizedconcanavalinatreatedinwatercontentcontrolledhydratedionicliquids AT kimiyoshiichida renaturationoflyophilizedconcanavalinatreatedinwatercontentcontrolledhydratedionicliquids |
_version_ |
1724372171455725568 |