Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and...
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doaj-10ecae0d3b1c4928b330f670ac49ca832020-12-24T00:03:48ZengMDPI AGFoods2304-81582021-12-0110181810.3390/foods10010018Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince LeavesVaidotas Zvikas0Ieva Urbanaviciute1Rasa Bernotiene2Deimante Kulakauskiene3Urte Morkunaite4Zbigniev Balion5Daiva Majiene6Mindaugas Liaudanskas7Pranas Viskelis8Aiste Jekabsone9Valdas Jakstas10Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaLaboratory of Biochemistry and Technology, Institute for Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kauno str. 30, LT-54333 Babtai, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Eivenių str. 4, LT-50161 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Eivenių str. 4, LT-50161 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, LithuaniaGlioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ leaf extracts on glioblastoma C6 and HROG36 cells. As identified by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry, the extracts contained three prevailing groups of phenols: hydroxycinnamic acid derivatives; flavan-3-ols; and flavonols. Sixteen phenols were detected; the predominant compound was chlorogenic acid. The sum of detected phenols varied significantly between the cultivars ranging from 9322 µg/g (‘Rondo’) to 17,048 µg/g DW (‘Darius’). Incubation with the extracts decreased the viability of glioblastoma HROG36 cells with an efficiency similar to temozolomide, a drug used for glioblastoma treatment. In the case of C6 glioblastoma cells, the extracts were even more efficient than temozolomide. Interestingly, primary cerebellar neuronal-glial cells were significantly less sensitive to the extracts compared to the cancer cell lines. The results showed that JQ leaf ethanol extracts are rich in phenolic compounds, can efficiently reduce glioblastoma cell viability while preserving non-cancerous cells, and are worth further investigations as potential anticancer drugs.https://www.mdpi.com/2304-8158/10/1/18<i>Chaenomeles japonica</i> leavesphenolic compoundsglioblastomaanticancer activity |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vaidotas Zvikas Ieva Urbanaviciute Rasa Bernotiene Deimante Kulakauskiene Urte Morkunaite Zbigniev Balion Daiva Majiene Mindaugas Liaudanskas Pranas Viskelis Aiste Jekabsone Valdas Jakstas |
spellingShingle |
Vaidotas Zvikas Ieva Urbanaviciute Rasa Bernotiene Deimante Kulakauskiene Urte Morkunaite Zbigniev Balion Daiva Majiene Mindaugas Liaudanskas Pranas Viskelis Aiste Jekabsone Valdas Jakstas Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves Foods <i>Chaenomeles japonica</i> leaves phenolic compounds glioblastoma anticancer activity |
author_facet |
Vaidotas Zvikas Ieva Urbanaviciute Rasa Bernotiene Deimante Kulakauskiene Urte Morkunaite Zbigniev Balion Daiva Majiene Mindaugas Liaudanskas Pranas Viskelis Aiste Jekabsone Valdas Jakstas |
author_sort |
Vaidotas Zvikas |
title |
Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves |
title_short |
Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves |
title_full |
Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves |
title_fullStr |
Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves |
title_full_unstemmed |
Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves |
title_sort |
investigation of phenolic composition and anticancer properties of ethanolic extracts of japanese quince leaves |
publisher |
MDPI AG |
series |
Foods |
issn |
2304-8158 |
publishDate |
2021-12-01 |
description |
Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ leaf extracts on glioblastoma C6 and HROG36 cells. As identified by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry, the extracts contained three prevailing groups of phenols: hydroxycinnamic acid derivatives; flavan-3-ols; and flavonols. Sixteen phenols were detected; the predominant compound was chlorogenic acid. The sum of detected phenols varied significantly between the cultivars ranging from 9322 µg/g (‘Rondo’) to 17,048 µg/g DW (‘Darius’). Incubation with the extracts decreased the viability of glioblastoma HROG36 cells with an efficiency similar to temozolomide, a drug used for glioblastoma treatment. In the case of C6 glioblastoma cells, the extracts were even more efficient than temozolomide. Interestingly, primary cerebellar neuronal-glial cells were significantly less sensitive to the extracts compared to the cancer cell lines. The results showed that JQ leaf ethanol extracts are rich in phenolic compounds, can efficiently reduce glioblastoma cell viability while preserving non-cancerous cells, and are worth further investigations as potential anticancer drugs. |
topic |
<i>Chaenomeles japonica</i> leaves phenolic compounds glioblastoma anticancer activity |
url |
https://www.mdpi.com/2304-8158/10/1/18 |
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