Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves.
Tomato gray mold disease, caused by Botrytis cinerea, is a serious disease in tomato. Clonostachys rosea is an antagonistic microorganism to B. cinerea. To investigate the induced resistance mechanism of C. rosea, we examined the effects of these microorganisms on tomato leaves, along with changes i...
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doaj-110acc2492e2422484b7bbb00d37114d2020-11-25T01:18:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10269010.1371/journal.pone.0102690Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves.Liana Dalcantara Ongouya MouekoubaLili ZhangXin GuanXiuling ChenHongyu ChenJian ZhangJunfeng ZhangJingfu LiYijun YangAoxue WangTomato gray mold disease, caused by Botrytis cinerea, is a serious disease in tomato. Clonostachys rosea is an antagonistic microorganism to B. cinerea. To investigate the induced resistance mechanism of C. rosea, we examined the effects of these microorganisms on tomato leaves, along with changes in the activities of three defense enzymes (PAL, PPO, GST), second messengers (NO, H2O2, O2(-)) and phytohormones (IAA, ABA, GA3, ZT, MeJA, SA and C2H4). Compared to the control, all treatments induced higher levels of PAL, PPO and GST activity in tomato leaves and increased NO, SA and GA3 levels. The expression of WRKY and MAPK, two important transcription factors in plant disease resistance, was upregulated in C. rosea- and C. rosea plus B. cinerea-treated samples. Two-dimensional gel electrophoresis analysis showed that two abundant proteins were present in the C. rosea plus B. cinerea-treated samples but not in the other samples. These proteins were determined (by mass spectrum analysis) to be LEXYL2 (β-xylosidase) and ATP synthase CF1 alpha subunit. Therefore, C. rosea plus B. cinerea treatment induces gray mold resistance in tomato. This study provides a basis for elucidating the mechanism of C. rosea as a biocontrol agent.http://europepmc.org/articles/PMC4111289?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liana Dalcantara Ongouya Mouekouba Lili Zhang Xin Guan Xiuling Chen Hongyu Chen Jian Zhang Junfeng Zhang Jingfu Li Yijun Yang Aoxue Wang |
spellingShingle |
Liana Dalcantara Ongouya Mouekouba Lili Zhang Xin Guan Xiuling Chen Hongyu Chen Jian Zhang Junfeng Zhang Jingfu Li Yijun Yang Aoxue Wang Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. PLoS ONE |
author_facet |
Liana Dalcantara Ongouya Mouekouba Lili Zhang Xin Guan Xiuling Chen Hongyu Chen Jian Zhang Junfeng Zhang Jingfu Li Yijun Yang Aoxue Wang |
author_sort |
Liana Dalcantara Ongouya Mouekouba |
title |
Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. |
title_short |
Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. |
title_full |
Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. |
title_fullStr |
Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. |
title_full_unstemmed |
Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. |
title_sort |
analysis of clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Tomato gray mold disease, caused by Botrytis cinerea, is a serious disease in tomato. Clonostachys rosea is an antagonistic microorganism to B. cinerea. To investigate the induced resistance mechanism of C. rosea, we examined the effects of these microorganisms on tomato leaves, along with changes in the activities of three defense enzymes (PAL, PPO, GST), second messengers (NO, H2O2, O2(-)) and phytohormones (IAA, ABA, GA3, ZT, MeJA, SA and C2H4). Compared to the control, all treatments induced higher levels of PAL, PPO and GST activity in tomato leaves and increased NO, SA and GA3 levels. The expression of WRKY and MAPK, two important transcription factors in plant disease resistance, was upregulated in C. rosea- and C. rosea plus B. cinerea-treated samples. Two-dimensional gel electrophoresis analysis showed that two abundant proteins were present in the C. rosea plus B. cinerea-treated samples but not in the other samples. These proteins were determined (by mass spectrum analysis) to be LEXYL2 (β-xylosidase) and ATP synthase CF1 alpha subunit. Therefore, C. rosea plus B. cinerea treatment induces gray mold resistance in tomato. This study provides a basis for elucidating the mechanism of C. rosea as a biocontrol agent. |
url |
http://europepmc.org/articles/PMC4111289?pdf=render |
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