Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily
Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy...
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doaj-11181ea8741d40fb8b56a0df82e19bf42021-06-01T01:16:46ZengMDPI AGAgriculture2077-04722021-05-011149649610.3390/agriculture11060496Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in SicilyStefano Panno0Andrea Giovanni Caruso1Sofia Bertacca2Antonino Pisciotta3Rosario Di Lorenzo4Serafino Marchione5Slavica Matić6Salvatore Davino7Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, ItalyOsservatorio per le Malattie delle Piante di Palermo (OMP), Via Uditore 15, 90145 Palermo, ItalyInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), 10151 Turin, ItalyDepartment of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, ItalyGrapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy), and its genetic structure and molecular variability were studied. In detail, a total of 617 grapevine samples of 11 autochthonous grapevine cultivars were collected in 20 commercial vineyards. Preliminary screening by serological (DAS-ELISA) and molecular (RT-PCR) analyses for ArMV (arabis mosaic virus) and GFLV detection were conducted. Results obtained showed the absence of ArMV in all the samples analyzed, while 48 out of 617 samples gave positive results to GFLV, for a total of 9 out of 11 cultivars analyzed. Phylogenetic analyses carried out on the GFLV-CP gene of 18 Sicilian GFLV sequences selected in this study showed a certain degree of variability among the Sicilian isolates, suggesting a different origin, probably as a consequence of the continuous interchange of GFLV-infected propagating material with other Italian regions or viticultural areas located in other countries.https://www.mdpi.com/2077-0472/11/6/496GFLVgrapevine diseasemolecular variabilityDAS-ELISART-PCRphylogenetic analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefano Panno Andrea Giovanni Caruso Sofia Bertacca Antonino Pisciotta Rosario Di Lorenzo Serafino Marchione Slavica Matić Salvatore Davino |
spellingShingle |
Stefano Panno Andrea Giovanni Caruso Sofia Bertacca Antonino Pisciotta Rosario Di Lorenzo Serafino Marchione Slavica Matić Salvatore Davino Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily Agriculture GFLV grapevine disease molecular variability DAS-ELISA RT-PCR phylogenetic analysis |
author_facet |
Stefano Panno Andrea Giovanni Caruso Sofia Bertacca Antonino Pisciotta Rosario Di Lorenzo Serafino Marchione Slavica Matić Salvatore Davino |
author_sort |
Stefano Panno |
title |
Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily |
title_short |
Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily |
title_full |
Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily |
title_fullStr |
Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily |
title_full_unstemmed |
Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily |
title_sort |
genetic structure and molecular variability of grapevine fanleaf virus in sicily |
publisher |
MDPI AG |
series |
Agriculture |
issn |
2077-0472 |
publishDate |
2021-05-01 |
description |
Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy), and its genetic structure and molecular variability were studied. In detail, a total of 617 grapevine samples of 11 autochthonous grapevine cultivars were collected in 20 commercial vineyards. Preliminary screening by serological (DAS-ELISA) and molecular (RT-PCR) analyses for ArMV (arabis mosaic virus) and GFLV detection were conducted. Results obtained showed the absence of ArMV in all the samples analyzed, while 48 out of 617 samples gave positive results to GFLV, for a total of 9 out of 11 cultivars analyzed. Phylogenetic analyses carried out on the GFLV-CP gene of 18 Sicilian GFLV sequences selected in this study showed a certain degree of variability among the Sicilian isolates, suggesting a different origin, probably as a consequence of the continuous interchange of GFLV-infected propagating material with other Italian regions or viticultural areas located in other countries. |
topic |
GFLV grapevine disease molecular variability DAS-ELISA RT-PCR phylogenetic analysis |
url |
https://www.mdpi.com/2077-0472/11/6/496 |
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