Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study
Background: Oral squamous cell carcinoma (OSCC) is a devastating disease that is usually associated with a dense associated inflammatory infiltrate. Characterizing tumor-associated inflammation is critical to understand the pathogenies of tumor development and progression.Methods: We have tested a p...
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doaj-111af8b8d561439bb0ab0690fae247a32021-09-21T14:29:07ZengFrontiers Media S.A.Frontiers in Oral Health2673-48422021-09-01210.3389/froh.2021.740469740469Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot StudyCatherine Laliberté0Nicole Ng1Denise Eymael2Kevin Higgins3Aiman Ali4Alex Kiss5Grace Bradley6Grace Bradley7Marco A. O. Magalhaes8Marco A. O. Magalhaes9Marco A. O. Magalhaes10Oral Pathology and Oral Medicine, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaCancer Invasion and Metastasis Laboratory, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaCancer Invasion and Metastasis Laboratory, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaDepartment of Otolaryngology-Head and Neck Surgery, Odette Cancer Centre, Sunnybrook Health Sciences Centre, Toronto, ON, CanadaCancer Invasion and Metastasis Laboratory, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaEvaluative Clinical Sciences, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, ON, CanadaOral Pathology and Oral Medicine, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaDental and Maxillofacial Sciences Department, Sunnybrook Health Sciences Centre, Toronto, ON, CanadaOral Pathology and Oral Medicine, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaCancer Invasion and Metastasis Laboratory, Faculty of Dentistry, University of Toronto, Toronto, ON, CanadaDental and Maxillofacial Sciences Department, Sunnybrook Health Sciences Centre, Toronto, ON, CanadaBackground: Oral squamous cell carcinoma (OSCC) is a devastating disease that is usually associated with a dense associated inflammatory infiltrate. Characterizing tumor-associated inflammation is critical to understand the pathogenies of tumor development and progression.Methods: We have tested a protocol to analyze tissue and salivary immune cells and mediators of 37 patients with OSCC at different stages and compared to eight chronic periodontitis patients and 24 healthy controls. Tissue analysis was based on fluorescent immunohistochemistry (FIHC) and inflammatory mediators were analyzed using a Luminex-based 30-Plex panel. Immune cells were analyzed using multichannel flow cytometry including CD45, CD66b, CD3, CD4, CD8, CD25, CD56, CD68, CD138, PD-1, and PD-L1.Results: We show an increase in OSCC-associated inflammation characterized by increased pro-inflammatory cytokines including IL-6, IL-8, TNFα, and GMCSF and increased salivary immune cells.Conclusion: We described a new method to analyze salivary inflammatory markers that can be used in future studies to monitor disease progression and prognosis.https://www.frontiersin.org/articles/10.3389/froh.2021.740469/fulloral cancerinflammationdysplasiasalivacancercytokine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Catherine Laliberté Nicole Ng Denise Eymael Kevin Higgins Aiman Ali Alex Kiss Grace Bradley Grace Bradley Marco A. O. Magalhaes Marco A. O. Magalhaes Marco A. O. Magalhaes |
spellingShingle |
Catherine Laliberté Nicole Ng Denise Eymael Kevin Higgins Aiman Ali Alex Kiss Grace Bradley Grace Bradley Marco A. O. Magalhaes Marco A. O. Magalhaes Marco A. O. Magalhaes Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study Frontiers in Oral Health oral cancer inflammation dysplasia saliva cancer cytokine |
author_facet |
Catherine Laliberté Nicole Ng Denise Eymael Kevin Higgins Aiman Ali Alex Kiss Grace Bradley Grace Bradley Marco A. O. Magalhaes Marco A. O. Magalhaes Marco A. O. Magalhaes |
author_sort |
Catherine Laliberté |
title |
Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study |
title_short |
Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study |
title_full |
Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study |
title_fullStr |
Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study |
title_full_unstemmed |
Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study |
title_sort |
characterization of oral squamous cell carcinoma associated inflammation: a pilot study |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oral Health |
issn |
2673-4842 |
publishDate |
2021-09-01 |
description |
Background: Oral squamous cell carcinoma (OSCC) is a devastating disease that is usually associated with a dense associated inflammatory infiltrate. Characterizing tumor-associated inflammation is critical to understand the pathogenies of tumor development and progression.Methods: We have tested a protocol to analyze tissue and salivary immune cells and mediators of 37 patients with OSCC at different stages and compared to eight chronic periodontitis patients and 24 healthy controls. Tissue analysis was based on fluorescent immunohistochemistry (FIHC) and inflammatory mediators were analyzed using a Luminex-based 30-Plex panel. Immune cells were analyzed using multichannel flow cytometry including CD45, CD66b, CD3, CD4, CD8, CD25, CD56, CD68, CD138, PD-1, and PD-L1.Results: We show an increase in OSCC-associated inflammation characterized by increased pro-inflammatory cytokines including IL-6, IL-8, TNFα, and GMCSF and increased salivary immune cells.Conclusion: We described a new method to analyze salivary inflammatory markers that can be used in future studies to monitor disease progression and prognosis. |
topic |
oral cancer inflammation dysplasia saliva cancer cytokine |
url |
https://www.frontiersin.org/articles/10.3389/froh.2021.740469/full |
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