A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR
A 25-year-old female presented to the infertility OPD with complaints of secondary infertility and pain lower abdomen with watery discharge for the past five days. She had history of undergoing hysterosalpingography in a private hospital ten days back. The interventions included drainage of pyometr...
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Tehran University of Medical Sciences
2012-09-01
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doaj-112b89f8f6a143119bf73e7502a221092021-04-02T12:18:17ZengTehran University of Medical SciencesJournal of Family and Reproductive Health1735-89491735-93922012-09-0163157A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCRMegha Singhal0Renu Tanwar1Ashok Kumar2Sudha Prasad31 IVF and Reproductive Biology Centre, Department of Obstetrics & Gynaecology, Maulana Azad MedicalIVF and Reproductive Biology Centre, Department of Obstetrics & Gynaecology, Maulana Azad Medical CoIVF and Reproductive Biology Centre, Department of Obstetrics & Gynaecology, Maulana Azad Medical CoIVF and Reproductive Biology Centre, Department of Obstetrics & Gynaecology, Maulana Azad Medical Co A 25-year-old female presented to the infertility OPD with complaints of secondary infertility and pain lower abdomen with watery discharge for the past five days. She had history of undergoing hysterosalpingography in a private hospital ten days back. The interventions included drainage of pyometra, endometrial biopsy for routine and AFB smear/ culture, confirmation of diagnosis by mRNA-based RT-PCR for detection of M. tuberculosis-specific 85B antigen gene, anti-tubercular therapy. Pyometra and tubo-ovarian masses disappeared and patient resumed her normal period post-treatment. Genital tuberculosis was confirmed by mRNA-based RT-PCR and the disease resolved after anti-tubercular therapy. We conclude that a combination of high degree of clinical suspicion and ‘high-precision' gene detection methods (e.g. mRNA) in culture-negative cases may be useful in diagnosis of genital tuberculosis, particularly in infertile patients presenting with pyometra post-hysterosalpingography. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/157PyometratuberculosismRNA-based RT-PCRendometrial biopsy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Megha Singhal Renu Tanwar Ashok Kumar Sudha Prasad |
spellingShingle |
Megha Singhal Renu Tanwar Ashok Kumar Sudha Prasad A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR Journal of Family and Reproductive Health Pyometra tuberculosis mRNA-based RT-PCR endometrial biopsy |
author_facet |
Megha Singhal Renu Tanwar Ashok Kumar Sudha Prasad |
author_sort |
Megha Singhal |
title |
A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR |
title_short |
A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR |
title_full |
A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR |
title_fullStr |
A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR |
title_full_unstemmed |
A Rare Case of Tuberculous Pyometra in a Young Infertile Female Confirmed by mRNA-based RT-PCR |
title_sort |
rare case of tuberculous pyometra in a young infertile female confirmed by mrna-based rt-pcr |
publisher |
Tehran University of Medical Sciences |
series |
Journal of Family and Reproductive Health |
issn |
1735-8949 1735-9392 |
publishDate |
2012-09-01 |
description |
A 25-year-old female presented to the infertility OPD with complaints of secondary infertility and pain lower abdomen with watery discharge for the past five days. She had history of undergoing hysterosalpingography in a private hospital ten days back. The interventions included drainage of pyometra, endometrial biopsy for routine and AFB smear/ culture, confirmation of diagnosis by mRNA-based RT-PCR for detection of M. tuberculosis-specific 85B antigen gene, anti-tubercular therapy. Pyometra and tubo-ovarian masses disappeared and patient resumed her normal period post-treatment. Genital tuberculosis was confirmed by mRNA-based RT-PCR and the disease resolved after anti-tubercular therapy. We conclude that a combination of high degree of clinical suspicion and ‘high-precision' gene detection methods (e.g. mRNA) in culture-negative cases may be useful in diagnosis of genital tuberculosis, particularly in infertile patients presenting with pyometra post-hysterosalpingography.
|
topic |
Pyometra tuberculosis mRNA-based RT-PCR endometrial biopsy |
url |
https://jfrh.tums.ac.ir/index.php/jfrh/article/view/157 |
work_keys_str_mv |
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