Virtual histology of multi-modal magnetic resonance imaging of cerebral cortex in young men

• Main Authors: Yash Patel, Jean Shin, Mark Drakesmith, John Evans, Zdenka Pausova, Tomas Paus
• Format: Article
• Language: English
• Published: Elsevier 2020-09-01
• Series: NeuroImage
• Subjects: Magnetic resonance imaging; Myelin water fraction; Relaxometry; Cerebral cortex; mcDESPOT imaging; Diffusion tensor imaging
• Online Access: http://www.sciencedirect.com/science/article/pii/S1053811920304547

Neurobiology underlying inter-regional variations - across the human cerebral cortex - in measures derived with multi-modal magnetic resonance imaging (MRI) is poorly understood. Here, we characterize inter-regional variations in a large number of such measures, including T1 and T2 relaxation times, myelin water fraction (MWF), T1w/T2w ratio, mean diffusivity (MD), fractional anisotropy (FA), magnetization transfer ratio (MTR) and cortical thickness. We then employ a virtual-histology approach and relate these inter-regional profiles to those in cell-specific gene expression. Virtual histology revealed that most MRI-derived measures, including T1, T2 relaxation time, MWF, T1w/T2w ratio, MTR, FA and cortical thickness, are associated with expression profiles of genes specific to CA1 pyramidal cells; these genes are enriched in biological processes related to dendritic arborisation. In addition, T2 relaxation time, MWF and T1w/T2w ratio are associated with oligodendrocyte-specific gene-expression profiles, supporting their use as measures sensitive to intra-cortical myelin. MWF contributes more variance than T1w/T2w ratio to the mean oligodendrocyte expression profile, suggesting greater sensitivity to myelin. These cell-specific MRI associations may help provide a framework for determining which MRI sequences to acquire in studies with specific neurobiological hypotheses.