Deep tissue culture and hemoculture in dogs with wounds and sepsis
ABSTRACT: Contaminated and infected wounds occur very frequently in veterinary medicine and can cause systemic inflammatory response syndrome, sepsis, and death. This study aimed to test the feasibility of collecting wound material by deep-tissue or punch biopsy for microbial culture, determine the...
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Colégio Brasileiro de Patologia Animal (CBPA)
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doaj-1157985cb60c4d548f9c33734b3062352020-11-24T20:58:43ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-515037121483149010.1590/s0100-736x2017001200020S0100-736X2017001201483Deep tissue culture and hemoculture in dogs with wounds and sepsisMônica V. Bahr AriasFlávia N. PadilhaMarcia R.E. PeruginiABSTRACT: Contaminated and infected wounds occur very frequently in veterinary medicine and can cause systemic inflammatory response syndrome, sepsis, and death. This study aimed to test the feasibility of collecting wound material by deep-tissue or punch biopsy for microbial culture, determine the frequency of bacteria in the wound(s) and blood cultures and the susceptibility of these microbes to antimicrobials, and evaluate clinical parameters that could be related to prognosis. Thirty dogs with wounds and signs of SIRS/sepsis were included in this study. Bacteria were isolated from all wounds and 41 bacterial isolates could be identified based on culture of the materials collected by punch biopsy; 53.66% of the isolates were gram-negative, mainly involving Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus spp., and 46.34% were gram-positive bacteria such as Streptococcus spp., Enterococcus spp., and Staphylococcus spp. The survival rate was 66.67%. Based on blood culture analysis, we identified bacteremia in seven patients, predominantly of gram-negative bacteria, which negatively affected patient survival, as six dogs died. Hypoglycemia (≤60mg/dL) and severe hyperglycemia (≥180mg/dL) also negatively affected survival as 23.33% of the hypo/hyperglycemic dogs died. Factors such as blood lactate level at admission and hematocrit levels, and mean arterial pressure were not significantly correlated with death or survival of the dogs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017001201483&lng=en&tlng=enTissue culturehemoculturedogssepsisbacteriabacteremiablood glucose |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mônica V. Bahr Arias Flávia N. Padilha Marcia R.E. Perugini |
spellingShingle |
Mônica V. Bahr Arias Flávia N. Padilha Marcia R.E. Perugini Deep tissue culture and hemoculture in dogs with wounds and sepsis Pesquisa Veterinária Brasileira Tissue culture hemoculture dogs sepsis bacteria bacteremia blood glucose |
author_facet |
Mônica V. Bahr Arias Flávia N. Padilha Marcia R.E. Perugini |
author_sort |
Mônica V. Bahr Arias |
title |
Deep tissue culture and hemoculture in dogs with wounds and sepsis |
title_short |
Deep tissue culture and hemoculture in dogs with wounds and sepsis |
title_full |
Deep tissue culture and hemoculture in dogs with wounds and sepsis |
title_fullStr |
Deep tissue culture and hemoculture in dogs with wounds and sepsis |
title_full_unstemmed |
Deep tissue culture and hemoculture in dogs with wounds and sepsis |
title_sort |
deep tissue culture and hemoculture in dogs with wounds and sepsis |
publisher |
Colégio Brasileiro de Patologia Animal (CBPA) |
series |
Pesquisa Veterinária Brasileira |
issn |
1678-5150 |
description |
ABSTRACT: Contaminated and infected wounds occur very frequently in veterinary medicine and can cause systemic inflammatory response syndrome, sepsis, and death. This study aimed to test the feasibility of collecting wound material by deep-tissue or punch biopsy for microbial culture, determine the frequency of bacteria in the wound(s) and blood cultures and the susceptibility of these microbes to antimicrobials, and evaluate clinical parameters that could be related to prognosis. Thirty dogs with wounds and signs of SIRS/sepsis were included in this study. Bacteria were isolated from all wounds and 41 bacterial isolates could be identified based on culture of the materials collected by punch biopsy; 53.66% of the isolates were gram-negative, mainly involving Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus spp., and 46.34% were gram-positive bacteria such as Streptococcus spp., Enterococcus spp., and Staphylococcus spp. The survival rate was 66.67%. Based on blood culture analysis, we identified bacteremia in seven patients, predominantly of gram-negative bacteria, which negatively affected patient survival, as six dogs died. Hypoglycemia (≤60mg/dL) and severe hyperglycemia (≥180mg/dL) also negatively affected survival as 23.33% of the hypo/hyperglycemic dogs died. Factors such as blood lactate level at admission and hematocrit levels, and mean arterial pressure were not significantly correlated with death or survival of the dogs. |
topic |
Tissue culture hemoculture dogs sepsis bacteria bacteremia blood glucose |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017001201483&lng=en&tlng=en |
work_keys_str_mv |
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1716784898051670016 |