Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes
Abstract Background Agastache rugosa (Fisch. & C.A.Mey.) Kuntze (Korean mint) is used to treat diverse types of human disorders in traditional medicine. In recent years, its non-fermented leaf extract (ARE) has been shown to possess protective properties against ultraviolet-B (UV-B) radiation-in...
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doaj-117a748ff069497abafc1f6da9ec02862020-11-25T03:29:39ZengBMCBMC Complementary and Alternative Medicine1472-68822018-06-0118111010.1186/s12906-018-2194-9Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytesDaehyun Shin0Yoonjin Lee1Yu-Hua Huang2Hye-Won Lim3Kyounghee Jang4Dae-Duk Kim5Chang-Jin Lim6R & D Center, Cosmocos CorporationR & D Center, Cosmocos CorporationR & D Center, Shebah Biotech Inc.R & D Center, Shebah Biotech Inc.R & D Center, Cosmocos CorporationCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National UniversityR & D Center, Shebah Biotech Inc.Abstract Background Agastache rugosa (Fisch. & C.A.Mey.) Kuntze (Korean mint) is used to treat diverse types of human disorders in traditional medicine. In recent years, its non-fermented leaf extract (ARE) has been shown to possess protective properties against ultraviolet-B (UV-B) radiation-induced photooxidative stress. The present work aimed to examine whether probiotic bacterial fermentation would potentiate the skin anti-photoaging activity of ARE or not, by comparing the protective properties of ARE and corresponding fermented extract (ARE-F) against UV-B radiation-induced photooxidative stress in HaCaT keratinocytes. Methods ARE-F was produced from ARE by the fermentation with Lactobacillus rhamnosus HK-9, a type of Gram-positive probiotic bacterial strain. Anti-photoaging activities were evaluated by analyzing reactive oxygen species (ROS), promatrix metalloproteinases (proMMPs), total glutathione (GSH) and total superoxide dismutase (SOD) in UV-B-irradiated HaCaT keratinocytes. Antiradical activity was determined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. Results ARE-F contained higher attenuating activity on the UV-B-induced ROS generation than ARE. Similarly, ARE-F was able to diminish the UV-B-induced proMMP-9 and -2 more effectively than ARE. ARE-F displayed higher tendencies to augment the UV-B-reduced total GSH content and SOD activity than ARE. However, there were no significant difference between ARE and ARE-F in ABTS radical scavenging activities. Conclusions The findings suggest that the UV-B radiation-protective activity of ARE is enhanced by probiotic bacterial fermentation, which might improve the therapeutic and cosmetic values of A. rugosa leaves.http://link.springer.com/article/10.1186/s12906-018-2194-9Agastache rugosaAnti-photoagingGlutathioneHaCaTMatrix metalloproteinase (MMP)Probiotic fermentation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daehyun Shin Yoonjin Lee Yu-Hua Huang Hye-Won Lim Kyounghee Jang Dae-Duk Kim Chang-Jin Lim |
spellingShingle |
Daehyun Shin Yoonjin Lee Yu-Hua Huang Hye-Won Lim Kyounghee Jang Dae-Duk Kim Chang-Jin Lim Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes BMC Complementary and Alternative Medicine Agastache rugosa Anti-photoaging Glutathione HaCaT Matrix metalloproteinase (MMP) Probiotic fermentation |
author_facet |
Daehyun Shin Yoonjin Lee Yu-Hua Huang Hye-Won Lim Kyounghee Jang Dae-Duk Kim Chang-Jin Lim |
author_sort |
Daehyun Shin |
title |
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes |
title_short |
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes |
title_full |
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes |
title_fullStr |
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes |
title_full_unstemmed |
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes |
title_sort |
probiotic fermentation augments the skin anti-photoaging properties of agastache rugosa through up-regulating antioxidant components in uv-b-irradiated hacat keratinocytes |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2018-06-01 |
description |
Abstract Background Agastache rugosa (Fisch. & C.A.Mey.) Kuntze (Korean mint) is used to treat diverse types of human disorders in traditional medicine. In recent years, its non-fermented leaf extract (ARE) has been shown to possess protective properties against ultraviolet-B (UV-B) radiation-induced photooxidative stress. The present work aimed to examine whether probiotic bacterial fermentation would potentiate the skin anti-photoaging activity of ARE or not, by comparing the protective properties of ARE and corresponding fermented extract (ARE-F) against UV-B radiation-induced photooxidative stress in HaCaT keratinocytes. Methods ARE-F was produced from ARE by the fermentation with Lactobacillus rhamnosus HK-9, a type of Gram-positive probiotic bacterial strain. Anti-photoaging activities were evaluated by analyzing reactive oxygen species (ROS), promatrix metalloproteinases (proMMPs), total glutathione (GSH) and total superoxide dismutase (SOD) in UV-B-irradiated HaCaT keratinocytes. Antiradical activity was determined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. Results ARE-F contained higher attenuating activity on the UV-B-induced ROS generation than ARE. Similarly, ARE-F was able to diminish the UV-B-induced proMMP-9 and -2 more effectively than ARE. ARE-F displayed higher tendencies to augment the UV-B-reduced total GSH content and SOD activity than ARE. However, there were no significant difference between ARE and ARE-F in ABTS radical scavenging activities. Conclusions The findings suggest that the UV-B radiation-protective activity of ARE is enhanced by probiotic bacterial fermentation, which might improve the therapeutic and cosmetic values of A. rugosa leaves. |
topic |
Agastache rugosa Anti-photoaging Glutathione HaCaT Matrix metalloproteinase (MMP) Probiotic fermentation |
url |
http://link.springer.com/article/10.1186/s12906-018-2194-9 |
work_keys_str_mv |
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