Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks
The objective of this study was to investigate the effects of low-protein diets with low digestibility of feed ingredients on intestinal damage and to explore whether the protease supplementation can alleviate the damage in Pekin ducks. A total of 576 Pekin ducklings (6 replicate pens, 16 ducks/pen)...
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Format: | Article |
Language: | English |
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Elsevier
2020-12-01
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Series: | Poultry Science |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912030763X |
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doaj-118033a1b519499ab00666b2fab58413 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Q.D. Wang S. Li K.Y. Zhang Y. Zhang S.P. Bai X.M. Ding J.P. Wang H.W. Peng G. Tian Y. Xuan Z.W. Su Q.F. Zeng |
spellingShingle |
Q.D. Wang S. Li K.Y. Zhang Y. Zhang S.P. Bai X.M. Ding J.P. Wang H.W. Peng G. Tian Y. Xuan Z.W. Su Q.F. Zeng Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks Poultry Science low-protein diet intestinal health Pekin duck protease serum free amino acid |
author_facet |
Q.D. Wang S. Li K.Y. Zhang Y. Zhang S.P. Bai X.M. Ding J.P. Wang H.W. Peng G. Tian Y. Xuan Z.W. Su Q.F. Zeng |
author_sort |
Q.D. Wang |
title |
Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks |
title_short |
Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks |
title_full |
Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks |
title_fullStr |
Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks |
title_full_unstemmed |
Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks |
title_sort |
protease supplementation attenuates the intestinal health damage caused by low-protein diets in pekin ducks |
publisher |
Elsevier |
series |
Poultry Science |
issn |
0032-5791 |
publishDate |
2020-12-01 |
description |
The objective of this study was to investigate the effects of low-protein diets with low digestibility of feed ingredients on intestinal damage and to explore whether the protease supplementation can alleviate the damage in Pekin ducks. A total of 576 Pekin ducklings (6 replicate pens, 16 ducks/pen) were randomly assigned to 6 dietary treatments (3 × 2 factorial arrangement) in a randomized complete block design. Factors were CP levels (13.5%, 15.5%, and 17.5%) and protease (0 or 20,000U/kg). Compared with the diets containing 17.5% CP, low-protein diets (13.5% CP) showed suppressed (P < 0.05) growth performance and feed intake (FI); reduced (P < 0.05) serum-free arginine, isoleucine, leucine, methionine, phenylalanine, valine, and proline as well as the cecal acetate and propionate concentration; increased (P < 0.05) plasma and ileal mucosal tumor necrosis factor-α (TNF-α) concentration; and downregulated (P < 0.05) mRNA expression of TNF-α, nuclear transcription factor-κb, interferon gamma, and Occludin in ileal mucosa. Irrespective of the dietary CP levels, protease supplementation significantly increased (P < 0.05) the serum-free glutamic acid concentration while decreasing (P < 0.05) the plasma endotoxin, IL-6, and the cecal isovalerate concentration. A significant interactive effect was observed between low-protein diets and protease supplementation (P < 0.05) on serum-free arginine concentration, the ratio of ileal villus height to crypt depth, and the IL-6 concentration in ileal mucosa. These results indicated that low-protein diets could damage intestinal integrity to induce systemic inflammation response and at last to suppress growth performance. Protease supplementation could partly attenuate the negative effects on gut health caused by low-protein diets in Pekin ducks. |
topic |
low-protein diet intestinal health Pekin duck protease serum free amino acid |
url |
http://www.sciencedirect.com/science/article/pii/S003257912030763X |
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doaj-118033a1b519499ab00666b2fab584132020-11-27T04:17:52ZengElsevierPoultry Science0032-57912020-12-01991266306642Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducksQ.D. Wang0S. Li1K.Y. Zhang2Y. Zhang3S.P. Bai4X.M. Ding5J.P. Wang6H.W. Peng7G. Tian8Y. Xuan9Z.W. Su10Q.F. Zeng11Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaMianyang Habio Bioengineering Co., Ltd., Mianyang 610000, ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, 611130 China; Corresponding author:The objective of this study was to investigate the effects of low-protein diets with low digestibility of feed ingredients on intestinal damage and to explore whether the protease supplementation can alleviate the damage in Pekin ducks. A total of 576 Pekin ducklings (6 replicate pens, 16 ducks/pen) were randomly assigned to 6 dietary treatments (3 × 2 factorial arrangement) in a randomized complete block design. Factors were CP levels (13.5%, 15.5%, and 17.5%) and protease (0 or 20,000U/kg). Compared with the diets containing 17.5% CP, low-protein diets (13.5% CP) showed suppressed (P < 0.05) growth performance and feed intake (FI); reduced (P < 0.05) serum-free arginine, isoleucine, leucine, methionine, phenylalanine, valine, and proline as well as the cecal acetate and propionate concentration; increased (P < 0.05) plasma and ileal mucosal tumor necrosis factor-α (TNF-α) concentration; and downregulated (P < 0.05) mRNA expression of TNF-α, nuclear transcription factor-κb, interferon gamma, and Occludin in ileal mucosa. Irrespective of the dietary CP levels, protease supplementation significantly increased (P < 0.05) the serum-free glutamic acid concentration while decreasing (P < 0.05) the plasma endotoxin, IL-6, and the cecal isovalerate concentration. A significant interactive effect was observed between low-protein diets and protease supplementation (P < 0.05) on serum-free arginine concentration, the ratio of ileal villus height to crypt depth, and the IL-6 concentration in ileal mucosa. These results indicated that low-protein diets could damage intestinal integrity to induce systemic inflammation response and at last to suppress growth performance. Protease supplementation could partly attenuate the negative effects on gut health caused by low-protein diets in Pekin ducks.http://www.sciencedirect.com/science/article/pii/S003257912030763Xlow-protein dietintestinal healthPekin duckproteaseserum free amino acid |