Evidentiation of Paramyosin (Sm-97) as a Modulating Antigen on Granulomatous Hypersensitivity to Schistosoma mansoni Eggs

• Main Authors: Hirsch Cristine, Carvalho-Queiroz Claudia, Franco Gloria R, Pena Sergio DJ, Simpson Andrew JG, Goes Alfredo M
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 1997-01-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Paramyosin; modulation; granuloma; Schistosoma mansoni
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500020

A Schistosoma mansoni adult worm anionic fraction (PIII) has previously been shown to protect mice against challenge infection and to reduce pulmonary and hepatic granulomatous hypersensitivity. Serum from PIII-immunized rabbit was used to screen a lgt11 cDNA library from S. mansoni adult worm in order to identify antigens capable of modulating granulomatous hypersensitivity. We obtained four clones with 400 (Sm-III.11), 900 (Sm-III.16), 1100 (Sm-III.10) and 1300 (Sm-III.12) bp of length. All clone-specific antibodies were able to recognize most of the PIII components. The sequence analysis showed that these clones presented high homology with S. mansoni paramyosin (Sm-97). These findings ascribe a new function to this antigen with an important role in modulation of granulomatous hypersensitivity to S. mansoni eggs