The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves
The morbidity and mortality rates from Pseudomonas aeruginosa infections are increasing, due to the development of drug-resistant strains. This study aimed to explore the secondary metabolites of endophytic fungi of Garcinia kola for their antibacterial activities against P. aeruginosa. The endoph...
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doaj-119e39659e5a44e98b49504727269ff02021-04-24T11:31:06ZengAin Shams UniversityNovel Research in Microbiology Journal2537-02862537-02942020-06-014384585510.21608/nrmj.2020.95326The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leavesDavid C. Nwobodo0Chibueze P. Ihekwereme1Chinedu J. Ikem2Festus B.C. Okoye3Department of Microbiology, Renaissance University, Ugbawka, Enugu State, NigeriaDepartment of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, NigeriaDepartment of Pharmaceutical Microbiology & Biotechnology, Madonna University, Elele, Rivers State, Nigeria, Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, NigeriaDepartment of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, NigeriaThe morbidity and mortality rates from Pseudomonas aeruginosa infections are increasing, due to the development of drug-resistant strains. This study aimed to explore the secondary metabolites of endophytic fungi of Garcinia kola for their antibacterial activities against P. aeruginosa. The endophytic fungi associated with healthy leaves of G. kola were isolated using the standard methods. These fungi were subjected to solid-state fermentation on rice media at 28oC for 21 d. The fungal secondary metabolites were extracted using ethyl acetate, and then concentrated under vacuum. The fungal crude extracts were screened for their antibacterial activities against clinical and laboratory strains of P. aeruginosa, using the agar diffusion method. The bioactive components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) analysis. Three endophytic fungi mainly; Aspergillus sp., Fusarium sp. and Colletotrichum sp. were isolated. At concentration of 1 mg/ml, extracts of the three fungi displayed antipseudomonal activities against all the isolates, except for a P. aeruginosa isolate recovered from urine. Results of the HPLC-DAD analysis revealed the presence of several active compounds such as; indole-3-acetic acid, phydroxybenzoic acid, and protocatechuic acid, among others in the fungal extracts. These compounds have been previously reported to have significant antimicrobial properties. This study reveals that endophytic fungi associated with G. kola leaves possess promising anti-pseudomonal potential.https://nrmj.journals.ekb.eg/article_95326_58e3a21bb2dd1b0b19a706a43e43637c.pdfpseudomonas aeruginosagarcinia kolaendophytic fungianti-pseudomonalsecondary metabolites |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David C. Nwobodo Chibueze P. Ihekwereme Chinedu J. Ikem Festus B.C. Okoye |
spellingShingle |
David C. Nwobodo Chibueze P. Ihekwereme Chinedu J. Ikem Festus B.C. Okoye The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves Novel Research in Microbiology Journal pseudomonas aeruginosa garcinia kola endophytic fungi anti-pseudomonal secondary metabolites |
author_facet |
David C. Nwobodo Chibueze P. Ihekwereme Chinedu J. Ikem Festus B.C. Okoye |
author_sort |
David C. Nwobodo |
title |
The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves |
title_short |
The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves |
title_full |
The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves |
title_fullStr |
The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves |
title_full_unstemmed |
The anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from Garcinia kola leaves |
title_sort |
anti-pseudomonal potentials of metabolites from some endophytic fungi isolated from garcinia kola leaves |
publisher |
Ain Shams University |
series |
Novel Research in Microbiology Journal |
issn |
2537-0286 2537-0294 |
publishDate |
2020-06-01 |
description |
The morbidity and mortality rates from Pseudomonas aeruginosa infections are increasing, due to the
development of drug-resistant strains. This study aimed to explore the secondary metabolites of endophytic fungi
of Garcinia kola for their antibacterial activities against P. aeruginosa. The endophytic fungi associated with
healthy leaves of G. kola were isolated using the standard methods. These fungi were subjected to solid-state
fermentation on rice media at 28oC for 21 d. The fungal secondary metabolites were extracted using ethyl acetate,
and then concentrated under vacuum. The fungal crude extracts were screened for their antibacterial activities
against clinical and laboratory strains of P. aeruginosa, using the agar diffusion method. The bioactive
components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array
Detector (HPLC-DAD) analysis. Three endophytic fungi mainly; Aspergillus sp., Fusarium sp. and
Colletotrichum sp. were isolated. At concentration of 1 mg/ml, extracts of the three fungi displayed antipseudomonal activities against all the isolates, except for a P. aeruginosa isolate recovered from urine. Results of
the HPLC-DAD analysis revealed the presence of several active compounds such as; indole-3-acetic acid, phydroxybenzoic acid, and protocatechuic acid, among others in the fungal extracts. These compounds have been
previously reported to have significant antimicrobial properties. This study reveals that endophytic fungi
associated with G. kola leaves possess promising anti-pseudomonal potential. |
topic |
pseudomonas aeruginosa garcinia kola endophytic fungi anti-pseudomonal secondary metabolites |
url |
https://nrmj.journals.ekb.eg/article_95326_58e3a21bb2dd1b0b19a706a43e43637c.pdf |
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