Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity.
<h4>Background</h4>The clinical significance of the quantitative value of antibodies in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains mostly unidentified. We investigated the dynamics and clinical implications of the SARS-CoV-2 antibody over time using th...
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doaj-11a62e0d0c7746f494003929a04b2df02021-07-15T04:30:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01166e025388910.1371/journal.pone.0253889Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity.Nuri LeeSeri JeongMin-Jeong ParkWonkeun Song<h4>Background</h4>The clinical significance of the quantitative value of antibodies in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains mostly unidentified. We investigated the dynamics and clinical implications of the SARS-CoV-2 antibody over time using three automated chemiluminescence immunoassays targeting either nucleocapsids or spikes.<h4>Methods</h4>A total of 126 specimens were collected from 23 patients with confirmed and indeterminate COVID-19 identified by molecular tests. SARS-CoV-2 antibody index was measured using SARS-CoV2 IgG reagent from Alinity (Abbott) and Access (Beckman Coulter) and SARS-CoV2 Total (IgG + IgM) from Atellica (Siemens).<h4>Results</h4>Three immunoassays showed strong correlations with each other (range of Pearson' s correlation coefficient (r) = 0.700-0.854, P < 0.001). Eleven (8.7%) specimens showed inconsistencies. SARS-CoV-2 IgG showed a statistically significantly higher value in patients with severe disease than that in non-severe disease patients (P < 0.001) and was significantly associated with clinical markers of disease severity.<h4>Conclusion</h4>The quantitative value of the SARS-CoV-2 IgG antibody measured using automated immunoassays is a significant indicator of clinical severity in patients with COVID-19.https://doi.org/10.1371/journal.pone.0253889 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nuri Lee Seri Jeong Min-Jeong Park Wonkeun Song |
spellingShingle |
Nuri Lee Seri Jeong Min-Jeong Park Wonkeun Song Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity. PLoS ONE |
author_facet |
Nuri Lee Seri Jeong Min-Jeong Park Wonkeun Song |
author_sort |
Nuri Lee |
title |
Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity. |
title_short |
Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity. |
title_full |
Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity. |
title_fullStr |
Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity. |
title_full_unstemmed |
Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity. |
title_sort |
comparison of three serological chemiluminescence immunoassays for sars-cov-2, and clinical significance of antibody index with disease severity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
<h4>Background</h4>The clinical significance of the quantitative value of antibodies in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains mostly unidentified. We investigated the dynamics and clinical implications of the SARS-CoV-2 antibody over time using three automated chemiluminescence immunoassays targeting either nucleocapsids or spikes.<h4>Methods</h4>A total of 126 specimens were collected from 23 patients with confirmed and indeterminate COVID-19 identified by molecular tests. SARS-CoV-2 antibody index was measured using SARS-CoV2 IgG reagent from Alinity (Abbott) and Access (Beckman Coulter) and SARS-CoV2 Total (IgG + IgM) from Atellica (Siemens).<h4>Results</h4>Three immunoassays showed strong correlations with each other (range of Pearson' s correlation coefficient (r) = 0.700-0.854, P < 0.001). Eleven (8.7%) specimens showed inconsistencies. SARS-CoV-2 IgG showed a statistically significantly higher value in patients with severe disease than that in non-severe disease patients (P < 0.001) and was significantly associated with clinical markers of disease severity.<h4>Conclusion</h4>The quantitative value of the SARS-CoV-2 IgG antibody measured using automated immunoassays is a significant indicator of clinical severity in patients with COVID-19. |
url |
https://doi.org/10.1371/journal.pone.0253889 |
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