Oral tolerance induction with altered forms of ovalbumin
As a T cell-dependent phenomenon, oral tolerance is not expected to depend necessarily on native configuration of antigens. We investigated the induction of oral tolerance with modified ovalbumin (Ova). Oral administration of heat-denatured (HD-Ova) and cyanogen bromide-degraded ovalbumin was less e...
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Associação Brasileira de Divulgação Científica
1998-01-01
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doaj-11a98c2c825447b1acde761df525b4fe2020-11-24T23:19:53ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73101998-01-01313381386Oral tolerance induction with altered forms of ovalbuminStransky B.Faria A.M.C.Vaz N.M.As a T cell-dependent phenomenon, oral tolerance is not expected to depend necessarily on native configuration of antigens. We investigated the induction of oral tolerance with modified ovalbumin (Ova). Oral administration of heat-denatured (HD-Ova) and cyanogen bromide-degraded ovalbumin was less effective than native Ova in inducing oral tolerance in B6D2F1 mice. HD-Ova was effective in suppressing delayed-type hypersensitivity (DTH) reactions but did not suppress specific antibody formation. Injection of Ova directly into the stomach, but not into the ileum or cecum, suppressed subsequent immunization to DTH reactions. Gavage with protease inhibitors (aprotinin or ovomucoid) before gavage with Ova was ineffective in blocking tolerance induction. Treatment with hydroxyurea to destroy cycling cells 24 h before gavage with Ova blocked oral tolerance induction and also the possibility to passively transfer tolerance to naive recipients with the serum of mice gavaged with Ova 1 h before. The implications of these findings about oral tolerance induction are discussedhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300009antigendigestionoral tolerancemouseovalbumin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stransky B. Faria A.M.C. Vaz N.M. |
spellingShingle |
Stransky B. Faria A.M.C. Vaz N.M. Oral tolerance induction with altered forms of ovalbumin Brazilian Journal of Medical and Biological Research antigen digestion oral tolerance mouse ovalbumin |
author_facet |
Stransky B. Faria A.M.C. Vaz N.M. |
author_sort |
Stransky B. |
title |
Oral tolerance induction with altered forms of ovalbumin |
title_short |
Oral tolerance induction with altered forms of ovalbumin |
title_full |
Oral tolerance induction with altered forms of ovalbumin |
title_fullStr |
Oral tolerance induction with altered forms of ovalbumin |
title_full_unstemmed |
Oral tolerance induction with altered forms of ovalbumin |
title_sort |
oral tolerance induction with altered forms of ovalbumin |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 0034-7310 |
publishDate |
1998-01-01 |
description |
As a T cell-dependent phenomenon, oral tolerance is not expected to depend necessarily on native configuration of antigens. We investigated the induction of oral tolerance with modified ovalbumin (Ova). Oral administration of heat-denatured (HD-Ova) and cyanogen bromide-degraded ovalbumin was less effective than native Ova in inducing oral tolerance in B6D2F1 mice. HD-Ova was effective in suppressing delayed-type hypersensitivity (DTH) reactions but did not suppress specific antibody formation. Injection of Ova directly into the stomach, but not into the ileum or cecum, suppressed subsequent immunization to DTH reactions. Gavage with protease inhibitors (aprotinin or ovomucoid) before gavage with Ova was ineffective in blocking tolerance induction. Treatment with hydroxyurea to destroy cycling cells 24 h before gavage with Ova blocked oral tolerance induction and also the possibility to passively transfer tolerance to naive recipients with the serum of mice gavaged with Ova 1 h before. The implications of these findings about oral tolerance induction are discussed |
topic |
antigen digestion oral tolerance mouse ovalbumin |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300009 |
work_keys_str_mv |
AT stranskyb oraltoleranceinductionwithalteredformsofovalbumin AT fariaamc oraltoleranceinductionwithalteredformsofovalbumin AT vaznm oraltoleranceinductionwithalteredformsofovalbumin |
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