New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall.
β-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to el...
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doaj-11abf6702dcd422bb250e4f7c2eda27d2020-11-25T01:33:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2761410.1371/journal.pone.0027614New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall.Douglas W LowmanLara J WestDaniel W BeardenMichael F WempeTrevor D PowerHarry E EnsleyKen HaynesDavid L WilliamsMichael D Kruppaβ-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity.http://europepmc.org/articles/PMC3214063?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Douglas W Lowman Lara J West Daniel W Bearden Michael F Wempe Trevor D Power Harry E Ensley Ken Haynes David L Williams Michael D Kruppa |
spellingShingle |
Douglas W Lowman Lara J West Daniel W Bearden Michael F Wempe Trevor D Power Harry E Ensley Ken Haynes David L Williams Michael D Kruppa New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. PLoS ONE |
author_facet |
Douglas W Lowman Lara J West Daniel W Bearden Michael F Wempe Trevor D Power Harry E Ensley Ken Haynes David L Williams Michael D Kruppa |
author_sort |
Douglas W Lowman |
title |
New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
title_short |
New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
title_full |
New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
title_fullStr |
New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
title_full_unstemmed |
New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
title_sort |
new insights into the structure of (1→3,1→6)-β-d-glucan side chains in the candida glabrata cell wall. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
β-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity. |
url |
http://europepmc.org/articles/PMC3214063?pdf=render |
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