Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease
Background/Aims: We found recently that increasing renal epoxyeicosatrienoic acids (EETs) levels by blocking soluble epoxide hydrolase (sEH), an enzyme responsible for EETs degradation, shows renoprotective actions and retards the progression of chronic kidney disease (CKD) in Ren-2 transgenic hyper...
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2018-03-01
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doaj-11b36c3fd958496eb6b6610466ba58ff2020-11-25T02:34:40ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432018-03-0143232934910.1159/000487902487902Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney DiseaseVěra Čertíková ChábováPetr KujalPetra ŠkaroupkováZdeňka VarňourkováŠárka VackováZuzana HuskováSoňa KikerlováJanusz SadowskiElzbieta Kompanowska-JezierskaIwona BaranowskaSung Hee HwangBruce D. HammockJohn D. ImigVladimír TesařLudek ČervenkaBackground/Aims: We found recently that increasing renal epoxyeicosatrienoic acids (EETs) levels by blocking soluble epoxide hydrolase (sEH), an enzyme responsible for EETs degradation, shows renoprotective actions and retards the progression of chronic kidney disease (CKD) in Ren-2 transgenic hypertensive rats (TGR) after 5/6 renal ablation (5/6 NX). This prompted us to examine if additional protection is provided when sEH inhibitor is added to the standard renin-angiotensin system (RAS) blockade, specifically in rats with established CKD. Methods: For RAS blockade, an angiotensin-converting enzyme inhibitor along with an angiotensin II type receptor blocker was used. RAS blockade was compared to sEH inhibition added to the RAS blockade. Treatments were initiated 6 weeks after 5/6 NX in TGR and the follow-up period was 60 weeks. Results: Combined RAS and sEH blockade exhibited additional positive impact on the rat survival rate, further reduced albuminuria, further reduced glomerular and tubulointerstitial injury, and attenuated the decline in creatinine clearance when compared to 5/6 NX TGR subjected to RAS blockade alone. These additional beneficial actions were associated with normalization of the intrarenal EETs deficient and a further reduction of urinary angiotensinogen excretion. Conclusion: This study provides evidence that addition of pharmacological inhibition of sEH to RAS blockade in 5/6 NX TGR enhances renoprotection and retards progression of CKD, notably, when applied at an advanced stage.https://www.karger.com/Article/FullText/487902Chronic kidney disease5/6 nephrectomyHypertensionRenin-angiotensin systemEpoxyeicosatrienoic acidsSoluble epoxide hydrolase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Věra Čertíková Chábová Petr Kujal Petra Škaroupková Zdeňka Varňourková Šárka Vacková Zuzana Husková Soňa Kikerlová Janusz Sadowski Elzbieta Kompanowska-Jezierska Iwona Baranowska Sung Hee Hwang Bruce D. Hammock John D. Imig Vladimír Tesař Ludek Červenka |
spellingShingle |
Věra Čertíková Chábová Petr Kujal Petra Škaroupková Zdeňka Varňourková Šárka Vacková Zuzana Husková Soňa Kikerlová Janusz Sadowski Elzbieta Kompanowska-Jezierska Iwona Baranowska Sung Hee Hwang Bruce D. Hammock John D. Imig Vladimír Tesař Ludek Červenka Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease Kidney & Blood Pressure Research Chronic kidney disease 5/6 nephrectomy Hypertension Renin-angiotensin system Epoxyeicosatrienoic acids Soluble epoxide hydrolase |
author_facet |
Věra Čertíková Chábová Petr Kujal Petra Škaroupková Zdeňka Varňourková Šárka Vacková Zuzana Husková Soňa Kikerlová Janusz Sadowski Elzbieta Kompanowska-Jezierska Iwona Baranowska Sung Hee Hwang Bruce D. Hammock John D. Imig Vladimír Tesař Ludek Červenka |
author_sort |
Věra Čertíková Chábová |
title |
Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease |
title_short |
Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease |
title_full |
Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease |
title_fullStr |
Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease |
title_full_unstemmed |
Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease |
title_sort |
combined inhibition of soluble epoxide hydrolase and renin-angiotensin system exhibits superior renoprotection to renin-angiotensin system blockade in 5/6 nephrectomized ren-2 transgenic hypertensive rats with established chronic kidney disease |
publisher |
Karger Publishers |
series |
Kidney & Blood Pressure Research |
issn |
1420-4096 1423-0143 |
publishDate |
2018-03-01 |
description |
Background/Aims: We found recently that increasing renal epoxyeicosatrienoic acids (EETs) levels by blocking soluble epoxide hydrolase (sEH), an enzyme responsible for EETs degradation, shows renoprotective actions and retards the progression of chronic kidney disease (CKD) in Ren-2 transgenic hypertensive rats (TGR) after 5/6 renal ablation (5/6 NX). This prompted us to examine if additional protection is provided when sEH inhibitor is added to the standard renin-angiotensin system (RAS) blockade, specifically in rats with established CKD. Methods: For RAS blockade, an angiotensin-converting enzyme inhibitor along with an angiotensin II type receptor blocker was used. RAS blockade was compared to sEH inhibition added to the RAS blockade. Treatments were initiated 6 weeks after 5/6 NX in TGR and the follow-up period was 60 weeks. Results: Combined RAS and sEH blockade exhibited additional positive impact on the rat survival rate, further reduced albuminuria, further reduced glomerular and tubulointerstitial injury, and attenuated the decline in creatinine clearance when compared to 5/6 NX TGR subjected to RAS blockade alone. These additional beneficial actions were associated with normalization of the intrarenal EETs deficient and a further reduction of urinary angiotensinogen excretion. Conclusion: This study provides evidence that addition of pharmacological inhibition of sEH to RAS blockade in 5/6 NX TGR enhances renoprotection and retards progression of CKD, notably, when applied at an advanced stage. |
topic |
Chronic kidney disease 5/6 nephrectomy Hypertension Renin-angiotensin system Epoxyeicosatrienoic acids Soluble epoxide hydrolase |
url |
https://www.karger.com/Article/FullText/487902 |
work_keys_str_mv |
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