Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures
Abstract Background BNO 1016 is an ethanolic extract of a mixture of five herbs that has been sold in different formulations for decades in the European market and more recently, in the United States market as an over-the-counter treatment for rhinosinusitis. Previous studies indicated activation of...
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doaj-11bba76bdaca46f2a1b966befbd56c952021-04-18T11:30:40ZengSpringerOpenClinical Phytoscience2199-11972021-04-01711610.1186/s40816-021-00276-2Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial culturesAlan D. Workman0Ivy W. Maina1Vasiliki Triantafillou2Neil N. Patel3Charles C. L. Tong4Edward C. Kuan5David W. Kennedy6James N. Palmer7Nithin D. Adappa8Noam A. Cohen9Department of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaDepartment of Otorhinolaryngology: Head and Neck Surgery, University of PennsylvaniaAbstract Background BNO 1016 is an ethanolic extract of a mixture of five herbs that has been sold in different formulations for decades in the European market and more recently, in the United States market as an over-the-counter treatment for rhinosinusitis. Previous studies indicated activation of chloride secretion and increase in ciliary beat frequency by BNO 1016 but the functional consequences on mucociliary transport velocity and airway surface liquid homeostasis are unknown. This study intends to examine the effects of BNO 1016 on these properties in vitro. Results Human sinonasal epithelial cells were grown at an air-liquid interface, with addition of BNO 1016 basolaterally in each experiment. Polystyrene fluorescent microspheres were added to the apical surface of the culture, and distance traveled across the surface of the culture over a fixed time period was measured using live imaging. BNO 1016 concentrations of 50 μg/ml and 500 μg/ml were tested. Basolateral application of compound resulted in a non-dose-dependent increase in culture surface liquid height compared to controls at 30 min, and this effect persisted through the one-hour duration of the experiment (p < 0.01). Basolateral application of BNO 1016 also resulted in a non-dose-dependent increase in microsphere transport velocity at 45 and 60 min following compound application (p < 0.01). Conclusions Basolateral application of BNO 1016 at a concentration mimicking post-ingestion serum levels appears to elicit increases in cell culture surface liquid height and mucociliary clearance, as assessed by microsphere transport velocity. These properties can potentially be leveraged for therapeutic efficacy in diseases affecting mucus production and mucociliary transport.https://doi.org/10.1186/s40816-021-00276-2SinusitisCiliaTherapeuticsSinupretBNO 1016 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alan D. Workman Ivy W. Maina Vasiliki Triantafillou Neil N. Patel Charles C. L. Tong Edward C. Kuan David W. Kennedy James N. Palmer Nithin D. Adappa Noam A. Cohen |
spellingShingle |
Alan D. Workman Ivy W. Maina Vasiliki Triantafillou Neil N. Patel Charles C. L. Tong Edward C. Kuan David W. Kennedy James N. Palmer Nithin D. Adappa Noam A. Cohen Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures Clinical Phytoscience Sinusitis Cilia Therapeutics Sinupret BNO 1016 |
author_facet |
Alan D. Workman Ivy W. Maina Vasiliki Triantafillou Neil N. Patel Charles C. L. Tong Edward C. Kuan David W. Kennedy James N. Palmer Nithin D. Adappa Noam A. Cohen |
author_sort |
Alan D. Workman |
title |
Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures |
title_short |
Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures |
title_full |
Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures |
title_fullStr |
Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures |
title_full_unstemmed |
Effects of BNO 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures |
title_sort |
effects of bno 1016 on ciliary transport velocity and cell culture surface liquid height of sinonasal epithelial cultures |
publisher |
SpringerOpen |
series |
Clinical Phytoscience |
issn |
2199-1197 |
publishDate |
2021-04-01 |
description |
Abstract Background BNO 1016 is an ethanolic extract of a mixture of five herbs that has been sold in different formulations for decades in the European market and more recently, in the United States market as an over-the-counter treatment for rhinosinusitis. Previous studies indicated activation of chloride secretion and increase in ciliary beat frequency by BNO 1016 but the functional consequences on mucociliary transport velocity and airway surface liquid homeostasis are unknown. This study intends to examine the effects of BNO 1016 on these properties in vitro. Results Human sinonasal epithelial cells were grown at an air-liquid interface, with addition of BNO 1016 basolaterally in each experiment. Polystyrene fluorescent microspheres were added to the apical surface of the culture, and distance traveled across the surface of the culture over a fixed time period was measured using live imaging. BNO 1016 concentrations of 50 μg/ml and 500 μg/ml were tested. Basolateral application of compound resulted in a non-dose-dependent increase in culture surface liquid height compared to controls at 30 min, and this effect persisted through the one-hour duration of the experiment (p < 0.01). Basolateral application of BNO 1016 also resulted in a non-dose-dependent increase in microsphere transport velocity at 45 and 60 min following compound application (p < 0.01). Conclusions Basolateral application of BNO 1016 at a concentration mimicking post-ingestion serum levels appears to elicit increases in cell culture surface liquid height and mucociliary clearance, as assessed by microsphere transport velocity. These properties can potentially be leveraged for therapeutic efficacy in diseases affecting mucus production and mucociliary transport. |
topic |
Sinusitis Cilia Therapeutics Sinupret BNO 1016 |
url |
https://doi.org/10.1186/s40816-021-00276-2 |
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