Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa
Among the several sucrose-catabolizing enzymes, neutral/alkaline invertases (NINs) play crucial roles in developmental processes as well as environmental stress responses in higher plants. Despite the fact that NINs are essential enzymes for plant life, the NIN family and their evolutionary relation...
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2019-01-01
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Online Access: | http://dx.doi.org/10.1080/13102818.2019.1643784 |
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doaj-11bdc91b7e73474a96d37008d730f2ba2020-11-25T02:13:31ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-013311158116310.1080/13102818.2019.16437841643784Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapaSeung Hee Eom0Yeonggil Rim1Tae Kyung Hyun2Chungbuk National UniversityGyeongsang National UniversityChungbuk National UniversityAmong the several sucrose-catabolizing enzymes, neutral/alkaline invertases (NINs) play crucial roles in developmental processes as well as environmental stress responses in higher plants. Despite the fact that NINs are essential enzymes for plant life, the NIN family and their evolutionary relationships are poorly understood. Therefore, in this study, we identified 11 NINs in the Brassica rapa (Chinese cabbage; BraNINs) genome, and analyzed the evolutionary mechanisms of BraNIN genes. Evolution analysis suggested that the BraNIN genes were duplicated via a segmental duplication event originating 15.81–45.25 million years ago. Furthermore, two segmental duplicated pairs (BraNIN5/6 and BraNIN7/8) were subject to negative selection. Furthermore, expression analysis of BraNINs using RNA-sequencing data suggested various functions of BraNINs during responses to drought stress. Taken together, our comparative genomic analysis of NIN genes in B. rapa provides information that will assist future studies on sucrose metabolism in sinks and sources of higher plants.http://dx.doi.org/10.1080/13102818.2019.1643784neutral/alkaline invertasebrassica rapaduplicationdrought |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seung Hee Eom Yeonggil Rim Tae Kyung Hyun |
spellingShingle |
Seung Hee Eom Yeonggil Rim Tae Kyung Hyun Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa Biotechnology & Biotechnological Equipment neutral/alkaline invertase brassica rapa duplication drought |
author_facet |
Seung Hee Eom Yeonggil Rim Tae Kyung Hyun |
author_sort |
Seung Hee Eom |
title |
Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa |
title_short |
Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa |
title_full |
Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa |
title_fullStr |
Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa |
title_full_unstemmed |
Genome-wide identification and evolutionary analysis of neutral/alkaline invertases in Brassica rapa |
title_sort |
genome-wide identification and evolutionary analysis of neutral/alkaline invertases in brassica rapa |
publisher |
Taylor & Francis Group |
series |
Biotechnology & Biotechnological Equipment |
issn |
1310-2818 1314-3530 |
publishDate |
2019-01-01 |
description |
Among the several sucrose-catabolizing enzymes, neutral/alkaline invertases (NINs) play crucial roles in developmental processes as well as environmental stress responses in higher plants. Despite the fact that NINs are essential enzymes for plant life, the NIN family and their evolutionary relationships are poorly understood. Therefore, in this study, we identified 11 NINs in the Brassica rapa (Chinese cabbage; BraNINs) genome, and analyzed the evolutionary mechanisms of BraNIN genes. Evolution analysis suggested that the BraNIN genes were duplicated via a segmental duplication event originating 15.81–45.25 million years ago. Furthermore, two segmental duplicated pairs (BraNIN5/6 and BraNIN7/8) were subject to negative selection. Furthermore, expression analysis of BraNINs using RNA-sequencing data suggested various functions of BraNINs during responses to drought stress. Taken together, our comparative genomic analysis of NIN genes in B. rapa provides information that will assist future studies on sucrose metabolism in sinks and sources of higher plants. |
topic |
neutral/alkaline invertase brassica rapa duplication drought |
url |
http://dx.doi.org/10.1080/13102818.2019.1643784 |
work_keys_str_mv |
AT seungheeeom genomewideidentificationandevolutionaryanalysisofneutralalkalineinvertasesinbrassicarapa AT yeonggilrim genomewideidentificationandevolutionaryanalysisofneutralalkalineinvertasesinbrassicarapa AT taekyunghyun genomewideidentificationandevolutionaryanalysisofneutralalkalineinvertasesinbrassicarapa |
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1724904737389674496 |