Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study
The studies on microbiome in the human digestive tract indicate that fungi could also be one of the external factors affecting development of diabetes. The aim of this study was to evaluate the quantitative and qualitative mycobiome composition in the colon of the adults with type 1 (T1D), <i>...
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doaj-11c95a174c774770a54c55bccf1a5f332021-03-26T00:03:24ZengMDPI AGNutrients2072-66432021-03-01131066106610.3390/nu13041066Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot StudyDominika Salamon0Agnieszka Sroka-Oleksiak1Artur Gurgul2Zbigniew Arent3Magdalena Szopa4Małgorzata Bulanda5Maciej T. Małecki6Tomasz Gosiewski7Department of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, PolandCenter for Experimental and Innovative Medicine, University of Agriculture, Krakow1c Rędzina Street, 30-248 Krakow, PolandCenter for Experimental and Innovative Medicine, University of Agriculture, Krakow1c Rędzina Street, 30-248 Krakow, PolandDepartment of Metabolic Diseases, Faculty of Medicine, Jagiellonian University Medical College, 2 Jakubowskiego Street, 30-688 Krakow, PolandDepartment of Epidemiology of Infections, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Krakow, PolandDepartment of Metabolic Diseases, Faculty of Medicine, Jagiellonian University Medical College, 2 Jakubowskiego Street, 30-688 Krakow, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, PolandThe studies on microbiome in the human digestive tract indicate that fungi could also be one of the external factors affecting development of diabetes. The aim of this study was to evaluate the quantitative and qualitative mycobiome composition in the colon of the adults with type 1 (T1D), <i>n</i> = 26 and type 2 (T2D) diabetes, <i>n</i> = 24 compared to the control group, <i>n</i> = 26. The gut mycobiome was characterized in the stool samples using the analysis of the whole internal transcribed spacer (ITS) region of the fungal rDNA gene cluster by next-generation sequencing (NGS) with increased sensitivity. At the L2 (phylum) level, Basidiomycota fungi were predominant in all 3 study groups. Group T1D presented significantly lower number of Ascomycota compared to the T2D group, and at the L6 (genus) level, the T1D group presented significantly lower number of <i>Saccharomyces</i> genus compared to control and T2D groups. In the T1D group, a significant positive correlation between total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and fungi of the genus <i>Saccharomyces,</i> and in the T2D group, a negative correlation between the total cholesterol level and <i>Malassezia</i> genus was found. The obtained results seem to be a good foundation to extend the analysis of the relationship between individual genera and species of fungi and the parameters determining the metabolism of carbohydrates and lipids in the human body.https://www.mdpi.com/2072-6643/13/4/1066diabetesgut mycobiomenext-generation sequencing (NGS) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dominika Salamon Agnieszka Sroka-Oleksiak Artur Gurgul Zbigniew Arent Magdalena Szopa Małgorzata Bulanda Maciej T. Małecki Tomasz Gosiewski |
spellingShingle |
Dominika Salamon Agnieszka Sroka-Oleksiak Artur Gurgul Zbigniew Arent Magdalena Szopa Małgorzata Bulanda Maciej T. Małecki Tomasz Gosiewski Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study Nutrients diabetes gut mycobiome next-generation sequencing (NGS) |
author_facet |
Dominika Salamon Agnieszka Sroka-Oleksiak Artur Gurgul Zbigniew Arent Magdalena Szopa Małgorzata Bulanda Maciej T. Małecki Tomasz Gosiewski |
author_sort |
Dominika Salamon |
title |
Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study |
title_short |
Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study |
title_full |
Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study |
title_fullStr |
Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study |
title_full_unstemmed |
Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study |
title_sort |
analysis of the gut mycobiome in adult patients with type 1 and type 2 diabetes using next-generation sequencing (ngs) with increased sensitivity—pilot study |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2021-03-01 |
description |
The studies on microbiome in the human digestive tract indicate that fungi could also be one of the external factors affecting development of diabetes. The aim of this study was to evaluate the quantitative and qualitative mycobiome composition in the colon of the adults with type 1 (T1D), <i>n</i> = 26 and type 2 (T2D) diabetes, <i>n</i> = 24 compared to the control group, <i>n</i> = 26. The gut mycobiome was characterized in the stool samples using the analysis of the whole internal transcribed spacer (ITS) region of the fungal rDNA gene cluster by next-generation sequencing (NGS) with increased sensitivity. At the L2 (phylum) level, Basidiomycota fungi were predominant in all 3 study groups. Group T1D presented significantly lower number of Ascomycota compared to the T2D group, and at the L6 (genus) level, the T1D group presented significantly lower number of <i>Saccharomyces</i> genus compared to control and T2D groups. In the T1D group, a significant positive correlation between total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and fungi of the genus <i>Saccharomyces,</i> and in the T2D group, a negative correlation between the total cholesterol level and <i>Malassezia</i> genus was found. The obtained results seem to be a good foundation to extend the analysis of the relationship between individual genera and species of fungi and the parameters determining the metabolism of carbohydrates and lipids in the human body. |
topic |
diabetes gut mycobiome next-generation sequencing (NGS) |
url |
https://www.mdpi.com/2072-6643/13/4/1066 |
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