In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions
Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> ssp. infections are associated with high rates of mortality, especially in infants in developing countries. Due to increasing levels of global antibiotic resistance exhibited by many pathogenic organisms, alte...
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doaj-11c9cbe639a14e5e865b494fd0c399d42021-09-26T00:43:34ZengMDPI AGMicroorganisms2076-26072021-09-0191869186910.3390/microorganisms9091869In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric ConditionsJoanna Kaczorowska0Eoghan Casey1Gabriele A. Lugli2Marco Ventura3David J. Clarke4Douwe van Sinderen5Jennifer Mahony6School of Microbiology, University College Cork, T12 YN60 Cork, IrelandSchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandLaboratory of Probiogenomics, Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43121 Parma, ItalyLaboratory of Probiogenomics, Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43121 Parma, ItalySchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandSchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandSchool of Microbiology, University College Cork, T12 YN60 Cork, IrelandEnterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> ssp. infections are associated with high rates of mortality, especially in infants in developing countries. Due to increasing levels of global antibiotic resistance exhibited by many pathogenic organisms, alternative strategies to combat such infections are urgently required. In this study, we evaluated the stability of five coliphages (four <i>Myoviridae</i> and one <i>Siphoviridae</i> phage) over a range of pH conditions and in simulated gastric conditions. The <i>Myoviridae</i> phages were stable across the range of pH 2 to 7, while the <i>Siphoviridae</i> phage, JK16, exhibited higher sensitivity to low pH. A composite mixture of these five phages was tested in vivo in a <i>Galleria mellonella</i> model. The obtained data clearly shows potential in treating <i>E. coli</i> infections prophylactically.https://www.mdpi.com/2076-2607/9/9/1869phage therapyprophylacticinfections<i>Galleria mellonella</i>diarrheagenic <i>Escherichia coli</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joanna Kaczorowska Eoghan Casey Gabriele A. Lugli Marco Ventura David J. Clarke Douwe van Sinderen Jennifer Mahony |
spellingShingle |
Joanna Kaczorowska Eoghan Casey Gabriele A. Lugli Marco Ventura David J. Clarke Douwe van Sinderen Jennifer Mahony In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions Microorganisms phage therapy prophylactic infections <i>Galleria mellonella</i> diarrheagenic <i>Escherichia coli</i> |
author_facet |
Joanna Kaczorowska Eoghan Casey Gabriele A. Lugli Marco Ventura David J. Clarke Douwe van Sinderen Jennifer Mahony |
author_sort |
Joanna Kaczorowska |
title |
In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions |
title_short |
In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions |
title_full |
In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions |
title_fullStr |
In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions |
title_full_unstemmed |
In Vitro and In Vivo Assessment of the Potential of <i>Escherichia coli</i> Phages to Treat Infections and Survive Gastric Conditions |
title_sort |
in vitro and in vivo assessment of the potential of <i>escherichia coli</i> phages to treat infections and survive gastric conditions |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-09-01 |
description |
Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> ssp. infections are associated with high rates of mortality, especially in infants in developing countries. Due to increasing levels of global antibiotic resistance exhibited by many pathogenic organisms, alternative strategies to combat such infections are urgently required. In this study, we evaluated the stability of five coliphages (four <i>Myoviridae</i> and one <i>Siphoviridae</i> phage) over a range of pH conditions and in simulated gastric conditions. The <i>Myoviridae</i> phages were stable across the range of pH 2 to 7, while the <i>Siphoviridae</i> phage, JK16, exhibited higher sensitivity to low pH. A composite mixture of these five phages was tested in vivo in a <i>Galleria mellonella</i> model. The obtained data clearly shows potential in treating <i>E. coli</i> infections prophylactically. |
topic |
phage therapy prophylactic infections <i>Galleria mellonella</i> diarrheagenic <i>Escherichia coli</i> |
url |
https://www.mdpi.com/2076-2607/9/9/1869 |
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