Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer
Qingwei Wu, 1, 2 Lili Yu, 1, 2 Xiaoqing Lin, 1, 2 Qingzhu Zheng, 1, 2 Songgao Zhang, 1, 2 Dunyan Chen, 1, 2 Xiaojie Pan, 1, 3 Yi Huang 1, 2, 4 1Provincial Clinical College, Fujian Medical University, Fuzhou 350001, People’s Republic of China; 2Department of Clinical Laboratory,...
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doaj-11d1d2d7ce464aa9b6c4b99c357d2d8c2020-11-25T02:56:05ZengDove Medical PressCancer Management and Research1179-13222020-01-01Volume 1248549551265Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung CancerWu QYu LLin XZheng QZhang SChen DPan XHuang YQingwei Wu, 1, 2 Lili Yu, 1, 2 Xiaoqing Lin, 1, 2 Qingzhu Zheng, 1, 2 Songgao Zhang, 1, 2 Dunyan Chen, 1, 2 Xiaojie Pan, 1, 3 Yi Huang 1, 2, 4 1Provincial Clinical College, Fujian Medical University, Fuzhou 350001, People’s Republic of China; 2Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of China; 3Department of Thoracic Surgery, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of China; 4Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of ChinaCorrespondence: Yi HuangDepartment of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of ChinaEmail hyi8070@126.comPurpose: Circulating microRNAs (miRNAs) have shown the potential for non-invasive diagnosis of various types of malignancies at an early stage. The aim of the study was to explore the feasibility of a combination of 8 serum miRNAs related to non-small-cell lung cancer (NSCLC) with the corresponding serum exosomal miRNAs in early diagnosis for the patients with NSCLC.Methods: We measured 8 serum miRNAs and the corresponding serum exosomal miRNAs including miR-21-5p, miR-126-3p, miR-141-3p, miR-146a-5p, miR-155-5p, miR-222-3p, miR-223-3p, and miR-486-5p in 48 patients with early NSCLC at stages I/II, 32 patients with lung benign lesion (LBL), and 48 healthy control (HC) by quantitative real-time polymerase chain reaction (qRT-PCR).Results: The expression levels of 4 serum miRNAs including miR-21-5p, miR-141-3p, miR-222-3p, and miR-486-5p, and 2 serum exosomal miRNAs including miR-146a-5p and miR-486-5p in the early NSCLC group were significantly different from that in the LBL group and the HC group (P < 0.01). The areas under the receiver operating characteristic curves (AUC) of the 4 serum miRNAs and 2 serum exosomal miRNAs in the early NSCLC group were ≥ 0.697, of which serum exosomal miR-146a-5p and miR-486-5p were 0.813 and 0.886, respectively, and higher than that of the 4 serum miRNAs. Additionally, a combination of 4 serum miRNAs with 2 serum exosomal miRNAs improved the AUC to 0.960 for the patients with NSCLC at early stages, with a sensitivity of 85.42% and a specificity of 92.50%.Conclusion: This study suggests that serum exosomal miRNAs other than serum miRNAs might be preferable biomarkers for the patients with NSCLC at early stages, and a combination of serum miRNAs with serum exosomal miRNAs contributes to the further improvement of early diagnosis for NSCLC.Keywords: serum, exosome, microRNA, non-small-cell lung cancer, early diagnosishttps://www.dovepress.com/combination-of-serum-mirnas-with-serum-exosomal-mirnas-in-early-diagno-peer-reviewed-article-CMARserumexosomemicrornanon-small cell lung cancerearly diagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wu Q Yu L Lin X Zheng Q Zhang S Chen D Pan X Huang Y |
spellingShingle |
Wu Q Yu L Lin X Zheng Q Zhang S Chen D Pan X Huang Y Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer Cancer Management and Research serum exosome microrna non-small cell lung cancer early diagnosis |
author_facet |
Wu Q Yu L Lin X Zheng Q Zhang S Chen D Pan X Huang Y |
author_sort |
Wu Q |
title |
Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer |
title_short |
Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer |
title_full |
Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer |
title_fullStr |
Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer |
title_full_unstemmed |
Combination of Serum miRNAs with Serum Exosomal miRNAs in Early Diagnosis for Non-Small-Cell Lung Cancer |
title_sort |
combination of serum mirnas with serum exosomal mirnas in early diagnosis for non-small-cell lung cancer |
publisher |
Dove Medical Press |
series |
Cancer Management and Research |
issn |
1179-1322 |
publishDate |
2020-01-01 |
description |
Qingwei Wu, 1, 2 Lili Yu, 1, 2 Xiaoqing Lin, 1, 2 Qingzhu Zheng, 1, 2 Songgao Zhang, 1, 2 Dunyan Chen, 1, 2 Xiaojie Pan, 1, 3 Yi Huang 1, 2, 4 1Provincial Clinical College, Fujian Medical University, Fuzhou 350001, People’s Republic of China; 2Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of China; 3Department of Thoracic Surgery, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of China; 4Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of ChinaCorrespondence: Yi HuangDepartment of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, People’s Republic of ChinaEmail hyi8070@126.comPurpose: Circulating microRNAs (miRNAs) have shown the potential for non-invasive diagnosis of various types of malignancies at an early stage. The aim of the study was to explore the feasibility of a combination of 8 serum miRNAs related to non-small-cell lung cancer (NSCLC) with the corresponding serum exosomal miRNAs in early diagnosis for the patients with NSCLC.Methods: We measured 8 serum miRNAs and the corresponding serum exosomal miRNAs including miR-21-5p, miR-126-3p, miR-141-3p, miR-146a-5p, miR-155-5p, miR-222-3p, miR-223-3p, and miR-486-5p in 48 patients with early NSCLC at stages I/II, 32 patients with lung benign lesion (LBL), and 48 healthy control (HC) by quantitative real-time polymerase chain reaction (qRT-PCR).Results: The expression levels of 4 serum miRNAs including miR-21-5p, miR-141-3p, miR-222-3p, and miR-486-5p, and 2 serum exosomal miRNAs including miR-146a-5p and miR-486-5p in the early NSCLC group were significantly different from that in the LBL group and the HC group (P < 0.01). The areas under the receiver operating characteristic curves (AUC) of the 4 serum miRNAs and 2 serum exosomal miRNAs in the early NSCLC group were ≥ 0.697, of which serum exosomal miR-146a-5p and miR-486-5p were 0.813 and 0.886, respectively, and higher than that of the 4 serum miRNAs. Additionally, a combination of 4 serum miRNAs with 2 serum exosomal miRNAs improved the AUC to 0.960 for the patients with NSCLC at early stages, with a sensitivity of 85.42% and a specificity of 92.50%.Conclusion: This study suggests that serum exosomal miRNAs other than serum miRNAs might be preferable biomarkers for the patients with NSCLC at early stages, and a combination of serum miRNAs with serum exosomal miRNAs contributes to the further improvement of early diagnosis for NSCLC.Keywords: serum, exosome, microRNA, non-small-cell lung cancer, early diagnosis |
topic |
serum exosome microrna non-small cell lung cancer early diagnosis |
url |
https://www.dovepress.com/combination-of-serum-mirnas-with-serum-exosomal-mirnas-in-early-diagno-peer-reviewed-article-CMAR |
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