Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent
Microalgae are considered a potential source of valuable compounds for multiple purposes and are potential agents for bioremediation of aquatic environments contaminated with different pollutants. This work evaluates the use of agricultural waste, unsterilized and anaerobically digested, to produce...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-03-01
|
Series: | Plants |
Subjects: | |
Online Access: | https://www.mdpi.com/2223-7747/10/3/478 |
id |
doaj-11ecb5342e86498fa0dccca1692b135b |
---|---|
record_format |
Article |
spelling |
doaj-11ecb5342e86498fa0dccca1692b135b2021-03-04T00:05:20ZengMDPI AGPlants2223-77472021-03-011047847810.3390/plants10030478Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester EffluentElvira E. Ziganshina0Svetlana S. Bulynina1Ayrat M. Ziganshin2Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, RussiaMicroalgae are considered a potential source of valuable compounds for multiple purposes and are potential agents for bioremediation of aquatic environments contaminated with different pollutants. This work evaluates the use of agricultural waste, unsterilized and anaerobically digested, to produce biomass from a strain of <i>Chlorella sorokiniana</i>. Furthermore, the presence of bacteria in these wastes was investigated based on the bacterial 16S rRNA gene sequencing. The results showed a specific growth rate ranging between 0.82 and 1.45 day<sup>–1</sup>, while the final biomass yield in different digestate-containing treatments (bacterial-contaminated cultures) ranged between 0.33 and 0.50 g L<sup>−1</sup> day<sup>−1</sup>. Besides, substantial amounts of ammonium, phosphate, and sulfate were consumed by <i>C. sorokiniana</i> during the experimental period. The predominant bacteria that grew in the presence of <i>C. sorokiniana</i> in the effluent-containing treatments belonged to the genera <i>Chryseobacterium</i>, <i>Flavobacterium</i>, <i>Sphingomonas</i>, <i>Brevundimonas</i>, <i>Hydrogenophaga</i>, <i>Sphingobacterium</i>, and <i>Pseudomonas</i>. Therefore, this microalga can tolerate and grow in the presence of other microorganisms. Finally, these results show that anaerobically digested agricultural waste materials are a good substitute for growth media for green microalgae; however, phosphate and sulfate levels must also be controlled in the media to maintain adequate growth of microalgae.https://www.mdpi.com/2223-7747/10/3/478photobioreactoranaerobic digester effluentmicroalgal–bacterial polyculture<i>Chlorella sorokiniana</i>bacterial community structurenutrient removal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elvira E. Ziganshina Svetlana S. Bulynina Ayrat M. Ziganshin |
spellingShingle |
Elvira E. Ziganshina Svetlana S. Bulynina Ayrat M. Ziganshin Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent Plants photobioreactor anaerobic digester effluent microalgal–bacterial polyculture <i>Chlorella sorokiniana</i> bacterial community structure nutrient removal |
author_facet |
Elvira E. Ziganshina Svetlana S. Bulynina Ayrat M. Ziganshin |
author_sort |
Elvira E. Ziganshina |
title |
Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent |
title_short |
Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent |
title_full |
Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent |
title_fullStr |
Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent |
title_full_unstemmed |
Assessment of <i>Chlorella sorokiniana</i> Growth in Anaerobic Digester Effluent |
title_sort |
assessment of <i>chlorella sorokiniana</i> growth in anaerobic digester effluent |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2021-03-01 |
description |
Microalgae are considered a potential source of valuable compounds for multiple purposes and are potential agents for bioremediation of aquatic environments contaminated with different pollutants. This work evaluates the use of agricultural waste, unsterilized and anaerobically digested, to produce biomass from a strain of <i>Chlorella sorokiniana</i>. Furthermore, the presence of bacteria in these wastes was investigated based on the bacterial 16S rRNA gene sequencing. The results showed a specific growth rate ranging between 0.82 and 1.45 day<sup>–1</sup>, while the final biomass yield in different digestate-containing treatments (bacterial-contaminated cultures) ranged between 0.33 and 0.50 g L<sup>−1</sup> day<sup>−1</sup>. Besides, substantial amounts of ammonium, phosphate, and sulfate were consumed by <i>C. sorokiniana</i> during the experimental period. The predominant bacteria that grew in the presence of <i>C. sorokiniana</i> in the effluent-containing treatments belonged to the genera <i>Chryseobacterium</i>, <i>Flavobacterium</i>, <i>Sphingomonas</i>, <i>Brevundimonas</i>, <i>Hydrogenophaga</i>, <i>Sphingobacterium</i>, and <i>Pseudomonas</i>. Therefore, this microalga can tolerate and grow in the presence of other microorganisms. Finally, these results show that anaerobically digested agricultural waste materials are a good substitute for growth media for green microalgae; however, phosphate and sulfate levels must also be controlled in the media to maintain adequate growth of microalgae. |
topic |
photobioreactor anaerobic digester effluent microalgal–bacterial polyculture <i>Chlorella sorokiniana</i> bacterial community structure nutrient removal |
url |
https://www.mdpi.com/2223-7747/10/3/478 |
work_keys_str_mv |
AT elviraeziganshina assessmentofichlorellasorokinianaigrowthinanaerobicdigestereffluent AT svetlanasbulynina assessmentofichlorellasorokinianaigrowthinanaerobicdigestereffluent AT ayratmziganshin assessmentofichlorellasorokinianaigrowthinanaerobicdigestereffluent |
_version_ |
1724232318992777216 |