The use of clade‐specific PCR assays to identify novel nitrilase genes from environmental isolates

The use of clade‐specific PCR assays to identify novel nitrilase genes from environmental isolates

Abstract Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio‐pharmaceuticals and industrial applications facilitates the move towards green chemistry....

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Bibliographic Details
Main Authors: Tríona‐Marie Dooley‐Cullinane, Catherine O'Reilly, Bilal Aslam, David P. Weiner, David O'Neill, Erica Owens, Denise O'Meara, Lee Coffey
Format: Article
Language:English
Published: Wiley 2019-04-01
Series:MicrobiologyOpen
Subjects:
clade specific
degenerate primers
environmental isolates
gene detection
nitrilase
touchdown PCR
Online Access:https://doi.org/10.1002/mbo3.700

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