In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells
For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in w...
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doaj-120745d8896b445a9619bda13bd6095b2021-07-15T07:53:53ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-07-01910.3389/fchem.2021.709581709581In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor CellsShuo Gao0Lei Zhao1Zhiqiang Fan2Vikram D. Kodibagkar3Li Liu4Hanqin Wang5Hong Xu6Mingli Tu7Bifu Hu8Chuanbin Cao9Zhenjian Zhang10Jian-Xin Yu11Jian-Xin Yu12Center of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United StatesDepartment of Radiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, United StatesCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaCenter of Translational Medicine, Fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, Suizhou, ChinaBiomedical Research Institute, Hubei University of Medicine, Shiyan, ChinaFor wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping.https://www.frontiersin.org/articles/10.3389/fchem.2021.709581/fullβ-galactosidase detectionresponsive Fe-based 1H-MRI agentT1 and T2 relaxation mappingin vitro1H-MRI imaginglacZ gene reportersynthesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuo Gao Lei Zhao Zhiqiang Fan Vikram D. Kodibagkar Li Liu Hanqin Wang Hong Xu Mingli Tu Bifu Hu Chuanbin Cao Zhenjian Zhang Jian-Xin Yu Jian-Xin Yu |
spellingShingle |
Shuo Gao Lei Zhao Zhiqiang Fan Vikram D. Kodibagkar Li Liu Hanqin Wang Hong Xu Mingli Tu Bifu Hu Chuanbin Cao Zhenjian Zhang Jian-Xin Yu Jian-Xin Yu In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells Frontiers in Chemistry β-galactosidase detection responsive Fe-based 1H-MRI agent T1 and T2 relaxation mapping in vitro1H-MRI imaging lacZ gene reporter synthesis |
author_facet |
Shuo Gao Lei Zhao Zhiqiang Fan Vikram D. Kodibagkar Li Liu Hanqin Wang Hong Xu Mingli Tu Bifu Hu Chuanbin Cao Zhenjian Zhang Jian-Xin Yu Jian-Xin Yu |
author_sort |
Shuo Gao |
title |
In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells |
title_short |
In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells |
title_full |
In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells |
title_fullStr |
In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells |
title_full_unstemmed |
In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells |
title_sort |
in situ generated novel 1h mri reporter for β-galactosidase activity detection and visualization in living tumor cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Chemistry |
issn |
2296-2646 |
publishDate |
2021-07-01 |
description |
For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping. |
topic |
β-galactosidase detection responsive Fe-based 1H-MRI agent T1 and T2 relaxation mapping in vitro1H-MRI imaging lacZ gene reporter synthesis |
url |
https://www.frontiersin.org/articles/10.3389/fchem.2021.709581/full |
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