Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology
A new potent halophilic protease producer, Halobacterium sp. strain LBU50301 was isolated from salt-fermented fish samples (budu) and identified by phenotypic analysis, and 16S rDNA gene sequencing. Thereafter, sequential statistical strategy was used to optimize halophilic protease production from...
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doaj-122a90a81614474a9228683ab929fe1c2020-11-25T01:01:17ZengElsevierBiotechnology Reports2215-017X2016-06-0110C172810.1016/j.btre.2016.02.004Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodologyJulalak Chuprom0Preeyanuch Bovornreungroj1Mehraj Ahmad2Duangporn Kantachote3Sawitree Dueramae4Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandInstitute of Nutrition (INMU), Mahidol University, 999 Phutthamonthon 4 Rd., Salaya, Nakhon Pathom 73170, ThailandDepartment of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandA new potent halophilic protease producer, Halobacterium sp. strain LBU50301 was isolated from salt-fermented fish samples (budu) and identified by phenotypic analysis, and 16S rDNA gene sequencing. Thereafter, sequential statistical strategy was used to optimize halophilic protease production from Halobacterium sp. strain LBU50301 by shake-flask fermentation. The classical one-factor-at-a-time (OFAT) approach determined gelatin was the best nitrogen source. Based on Plackett–Burman (PB) experimental design; gelatin, MgSO4·7H2O, NaCl and pH significantly influenced the halophilic protease production. Central composite design (CCD) determined the optimum level of medium components. Subsequently, an 8.78-fold increase in corresponding halophilic protease yield (156.22 U/mL) was obtained, compared with that produced in the original medium (17.80 U/mL). Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values. An overall 13-fold increase in halophilic protease yield was achieved using a 3 L laboratory fermenter and optimized medium (231.33 U/mL).http://www.sciencedirect.com/science/article/pii/S2215017X16300066GelatinHalobacterium sp. strainHalophilic proteaseResponse surface methodology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julalak Chuprom Preeyanuch Bovornreungroj Mehraj Ahmad Duangporn Kantachote Sawitree Dueramae |
spellingShingle |
Julalak Chuprom Preeyanuch Bovornreungroj Mehraj Ahmad Duangporn Kantachote Sawitree Dueramae Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology Biotechnology Reports Gelatin Halobacterium sp. strain Halophilic protease Response surface methodology |
author_facet |
Julalak Chuprom Preeyanuch Bovornreungroj Mehraj Ahmad Duangporn Kantachote Sawitree Dueramae |
author_sort |
Julalak Chuprom |
title |
Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology |
title_short |
Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology |
title_full |
Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology |
title_fullStr |
Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology |
title_full_unstemmed |
Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology |
title_sort |
approach toward enhancement of halophilic protease production by halobacterium sp. strain lbu50301 using statistical design response surface methodology |
publisher |
Elsevier |
series |
Biotechnology Reports |
issn |
2215-017X |
publishDate |
2016-06-01 |
description |
A new potent halophilic protease producer, Halobacterium sp. strain LBU50301 was isolated from salt-fermented fish samples (budu) and identified by phenotypic analysis, and 16S rDNA gene sequencing. Thereafter, sequential statistical strategy was used to optimize halophilic protease production from Halobacterium sp. strain LBU50301 by shake-flask fermentation. The classical one-factor-at-a-time (OFAT) approach determined gelatin was the best nitrogen source. Based on Plackett–Burman (PB) experimental design; gelatin, MgSO4·7H2O, NaCl and pH significantly influenced the halophilic protease production. Central composite design (CCD) determined the optimum level of medium components. Subsequently, an 8.78-fold increase in corresponding halophilic protease yield (156.22 U/mL) was obtained, compared with that produced in the original medium (17.80 U/mL). Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values. An overall 13-fold increase in halophilic protease yield was achieved using a 3 L laboratory fermenter and optimized medium (231.33 U/mL). |
topic |
Gelatin Halobacterium sp. strain Halophilic protease Response surface methodology |
url |
http://www.sciencedirect.com/science/article/pii/S2215017X16300066 |
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