Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses
<p>Abstract</p> <p>Background</p> <p>The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, <it>SOD </it>gene, was isolated from a <it>Deschampsia antarctica </it>Desv. by cDNA library screening. The expression of SOD gene in the leaves of <it&...
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doaj-122c31d6d0c54a1a8b6c38fae016acfb2020-11-25T01:17:51ZengBMCBMC Research Notes1756-05002009-09-012119810.1186/1756-0500-2-198Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stressesGidekel ManuelMoraga AnaDinamarca JorgeSánchez-Venegas Jaime R<p>Abstract</p> <p>Background</p> <p>The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, <it>SOD </it>gene, was isolated from a <it>Deschampsia antarctica </it>Desv. by cDNA library screening. The expression of SOD gene in the leaves of <it>D. antarctica </it>was determined by RT-PCR and its differential expression of gene transcripts in conditions of cold and UV radiation stresses was revealed by northern blot.</p> <p>Findings</p> <p>The molecular characterization shows that <it>SOD </it>cDNA is 709 bp in length, which translates an ORF of 152 amino acids that correspond to a protein of predicted molecular mass of 15 kDa. The assay shows that the expression of <it>SOD </it>gene increases when <it>D. antarctica </it>is acclimatised to 4°C and exposed to UV radiation. These results indicate that the <it>SOD </it>gene of <it>D. antarctica </it>is involved in the antioxidative process triggered by oxidative stress induced by the conditions of environmental change in which they live.</p> <p>Conclusion</p> <p>The present results allow us to know the characteristics of Cu/ZnSOD gene from <it>D. antarctica </it>and understand that its expression is regulated by cold and UV radiation.</p> http://www.biomedcentral.com/1756-0500/2/198 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R |
spellingShingle |
Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses BMC Research Notes |
author_facet |
Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R |
author_sort |
Gidekel Manuel |
title |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses |
title_short |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses |
title_full |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses |
title_fullStr |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses |
title_full_unstemmed |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from <it>Deschampsia antarctica </it>and its expression regulated by cold and UV stresses |
title_sort |
molecular characterization of a cdna encoding cu/zn superoxide dismutase from <it>deschampsia antarctica </it>and its expression regulated by cold and uv stresses |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2009-09-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, <it>SOD </it>gene, was isolated from a <it>Deschampsia antarctica </it>Desv. by cDNA library screening. The expression of SOD gene in the leaves of <it>D. antarctica </it>was determined by RT-PCR and its differential expression of gene transcripts in conditions of cold and UV radiation stresses was revealed by northern blot.</p> <p>Findings</p> <p>The molecular characterization shows that <it>SOD </it>cDNA is 709 bp in length, which translates an ORF of 152 amino acids that correspond to a protein of predicted molecular mass of 15 kDa. The assay shows that the expression of <it>SOD </it>gene increases when <it>D. antarctica </it>is acclimatised to 4°C and exposed to UV radiation. These results indicate that the <it>SOD </it>gene of <it>D. antarctica </it>is involved in the antioxidative process triggered by oxidative stress induced by the conditions of environmental change in which they live.</p> <p>Conclusion</p> <p>The present results allow us to know the characteristics of Cu/ZnSOD gene from <it>D. antarctica </it>and understand that its expression is regulated by cold and UV radiation.</p> |
url |
http://www.biomedcentral.com/1756-0500/2/198 |
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