Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses
Gut microbiome plays an essential role in modulating host immune responses. However, little is known about the interaction of microbiota, their metabolites and relevant inflammatory responses in the gut. By treating the mice with three different antibiotics (enrofloxacin, vancomycin, and polymixin B...
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doaj-123dae748f1b46cab6cf844137bc828b2020-11-24T21:45:54ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-04-01910.3389/fcimb.2019.00099433220Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune ResponsesLin Sun0Lin Sun1Xiaoyan Zhang2Yuxiao Zhang3Kai Zheng4Qiaoyan Xiang5Ning Chen6Zhiyun Chen7Nan Zhang8Junping Zhu9Qiushui He10Qiushui He11Department of Medical Microbiology, Capital Medical University, Beijing, ChinaBeijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology, Capital Medical University, Beijing, ChinaDepartment of Medical Microbiology and Immunology, University of Turku, Turku, FinlandGut microbiome plays an essential role in modulating host immune responses. However, little is known about the interaction of microbiota, their metabolites and relevant inflammatory responses in the gut. By treating the mice with three different antibiotics (enrofloxacin, vancomycin, and polymixin B sulfate), we aimed to investigate the effects of different antibiotics exposure on gut microbiota, microbial metabolism, inflammation responses in the gut, and most importantly, pinpoint the underlying interactions between them. Although the administration of different antibiotics can lead to different effects on mouse models, the treatment did not affect the average body weight of the mice. A heavier caecum was observed in vancomycin treated mice. Treatment by these three antibiotics significantly up-regulated gene expression of various cytokines in the colon. Enrofloxacin treated mice seemed to have an increased Th1 response in the colon. However, such a difference was not found in mice treated by vancomycin or polymixin B sulfate. Vancomycin treatment induced significant changes in bacterial composition at phylum and family level and decreased richness and diversity at species level. Enrofloxacin treatment only induced changes in composition at family presenting as an increase in Prevotellaceae and Rikenellaceae and a decrease in Bacteroidaceae. However, no significant difference was observed after polymixin B sulfate treatment. When compared with the control group, significant metabolic shift was found in the enrofloxacin and vancomycin treated group. The metabolic changes mainly occurred in Valine, leucine, and isoleucine biosynthesis pathway and beta-Alanine metabolism in enrofloxacin treated group. For vancomycin treatment metabolic changes were mainly found in beta-Alanine metabolism and Alanine, aspartate and glutamate metabolism pathway. Moreover, modifications observed in the microbiota compositions were correlated with the metabolite concentrations. For example, concentration of pentadecanoic acid was positively correlated with richness of Rikenellaceae and Prevotellaceae and negatively correlated with Enterobacteriaceae. This study suggests that the antibiotic-induced changes in gut microbiota might contribute to the inflammation responses through the alternation of metabolic status, providing a novel insight regarding a complex network that integrates the different interactions between gut microbiota, metabolic functions, and immune responses in host.https://www.frontiersin.org/article/10.3389/fcimb.2019.00099/fullgut microbiomemicrobial metaboliteimmune responsecytokineantibioticco-correlation analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin Sun Lin Sun Xiaoyan Zhang Yuxiao Zhang Kai Zheng Qiaoyan Xiang Ning Chen Zhiyun Chen Nan Zhang Junping Zhu Qiushui He Qiushui He |
spellingShingle |
Lin Sun Lin Sun Xiaoyan Zhang Yuxiao Zhang Kai Zheng Qiaoyan Xiang Ning Chen Zhiyun Chen Nan Zhang Junping Zhu Qiushui He Qiushui He Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses Frontiers in Cellular and Infection Microbiology gut microbiome microbial metabolite immune response cytokine antibiotic co-correlation analysis |
author_facet |
Lin Sun Lin Sun Xiaoyan Zhang Yuxiao Zhang Kai Zheng Qiaoyan Xiang Ning Chen Zhiyun Chen Nan Zhang Junping Zhu Qiushui He Qiushui He |
author_sort |
Lin Sun |
title |
Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses |
title_short |
Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses |
title_full |
Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses |
title_fullStr |
Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses |
title_full_unstemmed |
Antibiotic-Induced Disruption of Gut Microbiota Alters Local Metabolomes and Immune Responses |
title_sort |
antibiotic-induced disruption of gut microbiota alters local metabolomes and immune responses |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2019-04-01 |
description |
Gut microbiome plays an essential role in modulating host immune responses. However, little is known about the interaction of microbiota, their metabolites and relevant inflammatory responses in the gut. By treating the mice with three different antibiotics (enrofloxacin, vancomycin, and polymixin B sulfate), we aimed to investigate the effects of different antibiotics exposure on gut microbiota, microbial metabolism, inflammation responses in the gut, and most importantly, pinpoint the underlying interactions between them. Although the administration of different antibiotics can lead to different effects on mouse models, the treatment did not affect the average body weight of the mice. A heavier caecum was observed in vancomycin treated mice. Treatment by these three antibiotics significantly up-regulated gene expression of various cytokines in the colon. Enrofloxacin treated mice seemed to have an increased Th1 response in the colon. However, such a difference was not found in mice treated by vancomycin or polymixin B sulfate. Vancomycin treatment induced significant changes in bacterial composition at phylum and family level and decreased richness and diversity at species level. Enrofloxacin treatment only induced changes in composition at family presenting as an increase in Prevotellaceae and Rikenellaceae and a decrease in Bacteroidaceae. However, no significant difference was observed after polymixin B sulfate treatment. When compared with the control group, significant metabolic shift was found in the enrofloxacin and vancomycin treated group. The metabolic changes mainly occurred in Valine, leucine, and isoleucine biosynthesis pathway and beta-Alanine metabolism in enrofloxacin treated group. For vancomycin treatment metabolic changes were mainly found in beta-Alanine metabolism and Alanine, aspartate and glutamate metabolism pathway. Moreover, modifications observed in the microbiota compositions were correlated with the metabolite concentrations. For example, concentration of pentadecanoic acid was positively correlated with richness of Rikenellaceae and Prevotellaceae and negatively correlated with Enterobacteriaceae. This study suggests that the antibiotic-induced changes in gut microbiota might contribute to the inflammation responses through the alternation of metabolic status, providing a novel insight regarding a complex network that integrates the different interactions between gut microbiota, metabolic functions, and immune responses in host. |
topic |
gut microbiome microbial metabolite immune response cytokine antibiotic co-correlation analysis |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2019.00099/full |
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