Rapamycin activates mammalian microautophagy

Rapamycin activates mammalian microautophagy

Autophagy-lysosome proteolysis is classified into macroautophagy (MA), microautophagy (mA) and chaperone-mediated autophagy (CMA). In contrast to MA and CMA, mA have been mainly studied in yeast. In 2011, mammalian mA was identified as a pathway to deliver cytosolic proteins into multivesicular bodi...

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Main Authors: Masahiro Sato, Takahiro Seki, Ayumu Konno, Hirokazu Hirai, Yuki Kurauchi, Akinori Hisatsune, Hiroshi Katsuki
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319340629
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spelling doaj-1259e534934d4baaa4deac5c42c6851b2020-11-25T02:46:51ZengElsevierJournal of Pharmacological Sciences1347-86132019-06-011402201204Rapamycin activates mammalian microautophagyMasahiro Sato0Takahiro Seki1Ayumu Konno2Hirokazu Hirai3Yuki Kurauchi4Akinori Hisatsune5Hiroshi Katsuki6Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, JapanDepartment of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan; Corresponding author. Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Fax: +81 96 371 4182.Department of Neurophysiology & Neural Repair, Gunma University Graduate School of Medicine, Maebashi, JapanDepartment of Neurophysiology & Neural Repair, Gunma University Graduate School of Medicine, Maebashi, JapanDepartment of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, JapanDepartment of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan; Priority Organization for Innovation and Excellence, Kumamoto University, Kumamoto, Japan; Program for Leading Graduate Schools “HIGO (Health Life Science: Interdisciplinary and Glocal Oriented) Program”, Kumamoto University, Kumamoto, JapanDepartment of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, JapanAutophagy-lysosome proteolysis is classified into macroautophagy (MA), microautophagy (mA) and chaperone-mediated autophagy (CMA). In contrast to MA and CMA, mA have been mainly studied in yeast. In 2011, mammalian mA was identified as a pathway to deliver cytosolic proteins into multivesicular bodies. However, its molecular mechanism is quite different from yeast mA. Using a cell-based method to evaluate mA and CMA, we revealed that rapamycin, an activator of yeast mA, significantly activated mammalian mA. Although rapamycin activates MA, mA was also activated by rapamycin in MA-deficient cells. These findings suggest that rapamycin is a first-identified activator of mammalian mA. Keywords: Microautophagy, Chaperone-mediated autophagy, Rapamycinhttp://www.sciencedirect.com/science/article/pii/S1347861319340629
collection DOAJ
language English
format Article
sources DOAJ
author Masahiro Sato
Takahiro Seki
Ayumu Konno
Hirokazu Hirai
Yuki Kurauchi
Akinori Hisatsune
Hiroshi Katsuki
spellingShingle Masahiro Sato
Takahiro Seki
Ayumu Konno
Hirokazu Hirai
Yuki Kurauchi
Akinori Hisatsune
Hiroshi Katsuki
Rapamycin activates mammalian microautophagy
Journal of Pharmacological Sciences
author_facet Masahiro Sato
Takahiro Seki
Ayumu Konno
Hirokazu Hirai
Yuki Kurauchi
Akinori Hisatsune
Hiroshi Katsuki
author_sort Masahiro Sato
title Rapamycin activates mammalian microautophagy
title_short Rapamycin activates mammalian microautophagy
title_full Rapamycin activates mammalian microautophagy
title_fullStr Rapamycin activates mammalian microautophagy
title_full_unstemmed Rapamycin activates mammalian microautophagy
title_sort rapamycin activates mammalian microautophagy
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2019-06-01
description Autophagy-lysosome proteolysis is classified into macroautophagy (MA), microautophagy (mA) and chaperone-mediated autophagy (CMA). In contrast to MA and CMA, mA have been mainly studied in yeast. In 2011, mammalian mA was identified as a pathway to deliver cytosolic proteins into multivesicular bodies. However, its molecular mechanism is quite different from yeast mA. Using a cell-based method to evaluate mA and CMA, we revealed that rapamycin, an activator of yeast mA, significantly activated mammalian mA. Although rapamycin activates MA, mA was also activated by rapamycin in MA-deficient cells. These findings suggest that rapamycin is a first-identified activator of mammalian mA. Keywords: Microautophagy, Chaperone-mediated autophagy, Rapamycin
url http://www.sciencedirect.com/science/article/pii/S1347861319340629
work_keys_str_mv AT masahirosato rapamycinactivatesmammalianmicroautophagy
AT takahiroseki rapamycinactivatesmammalianmicroautophagy
AT ayumukonno rapamycinactivatesmammalianmicroautophagy
AT hirokazuhirai rapamycinactivatesmammalianmicroautophagy
AT yukikurauchi rapamycinactivatesmammalianmicroautophagy
AT akinorihisatsune rapamycinactivatesmammalianmicroautophagy
AT hiroshikatsuki rapamycinactivatesmammalianmicroautophagy
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