Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia
Preeclampsia is a gestation-associated hypertensive syndrome that threatens the life and health of the mother and the child. The condition is presumably caused by systemic failure with a strong involvement of innate immunity. In particular, it has been associated with flexible phenotypes of macropha...
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doaj-126bf5ab2bb44b32af336512a27b67e02021-02-15T00:03:20ZengMDPI AGBiomedicines2227-90592021-02-01919119110.3390/biomedicines9020191Expression of Estrogen Receptor α by Decidual Macrophages in PreeclampsiaPolina Vishnyakova0Anastasiya Poltavets1Maria Nikitina2Konstantin Midiber3Liudmila Mikhaleva4Kamilla Muminova5Alena Potapova6Zulfiya Khodzhaeva7Alexey Pyregov8Andrey Elchaninov9Timur Fatkhudinov10Gennady Sukhikh11National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaScientific Research Institute of Human Morphology, 117418 Moscow, RussiaScientific Research Institute of Human Morphology, 117418 Moscow, RussiaScientific Research Institute of Human Morphology, 117418 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaPeoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117198 Moscow, RussiaPreeclampsia is a gestation-associated hypertensive syndrome that threatens the life and health of the mother and the child. The condition is presumably caused by systemic failure with a strong involvement of innate immunity. In particular, it has been associated with flexible phenotypes of macrophages, which depend on the molecules circulating in the blood and tissue fluid, such as cytokines and hormones. This study aimed at a comparative evaluation of pro-inflammatory (TNFα) and anti-inflammatory (CD206, MMP9, HGF) markers, as well as the levels of estrogen receptor α, expressed by decidual macrophages in normal pregnancy and in patients with early- and late-onset preeclampsia. The tissue samples of decidua basalis were examined by immunohistochemistry and Western blotting. Isolation of decidual macrophages and their characterization were performed using cultural methods, flow cytometry and real-time PCR. Over 50% of the isolated decidual macrophages were positive for the pan-macrophage marker CD68. In the early-onset preeclampsia group, the levels of estrogen receptor α in decidua were significantly decreased. Furthermore, significantly decreased levels of HGF and CD206 were observed in both preeclampsia groups compared with the control group. The observed downregulation of estrogen receptor α, HGF and CD206 may contribute to the balance of pro- and anti-inflammatory macrophages and thereby to pathogenesis of preeclampsia.https://www.mdpi.com/2227-9059/9/2/191macrophages, preeclampsia, decidua, estrogen receptors, polarization, inflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Polina Vishnyakova Anastasiya Poltavets Maria Nikitina Konstantin Midiber Liudmila Mikhaleva Kamilla Muminova Alena Potapova Zulfiya Khodzhaeva Alexey Pyregov Andrey Elchaninov Timur Fatkhudinov Gennady Sukhikh |
spellingShingle |
Polina Vishnyakova Anastasiya Poltavets Maria Nikitina Konstantin Midiber Liudmila Mikhaleva Kamilla Muminova Alena Potapova Zulfiya Khodzhaeva Alexey Pyregov Andrey Elchaninov Timur Fatkhudinov Gennady Sukhikh Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia Biomedicines macrophages, preeclampsia, decidua, estrogen receptors, polarization, inflammation |
author_facet |
Polina Vishnyakova Anastasiya Poltavets Maria Nikitina Konstantin Midiber Liudmila Mikhaleva Kamilla Muminova Alena Potapova Zulfiya Khodzhaeva Alexey Pyregov Andrey Elchaninov Timur Fatkhudinov Gennady Sukhikh |
author_sort |
Polina Vishnyakova |
title |
Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia |
title_short |
Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia |
title_full |
Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia |
title_fullStr |
Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia |
title_full_unstemmed |
Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia |
title_sort |
expression of estrogen receptor α by decidual macrophages in preeclampsia |
publisher |
MDPI AG |
series |
Biomedicines |
issn |
2227-9059 |
publishDate |
2021-02-01 |
description |
Preeclampsia is a gestation-associated hypertensive syndrome that threatens the life and health of the mother and the child. The condition is presumably caused by systemic failure with a strong involvement of innate immunity. In particular, it has been associated with flexible phenotypes of macrophages, which depend on the molecules circulating in the blood and tissue fluid, such as cytokines and hormones. This study aimed at a comparative evaluation of pro-inflammatory (TNFα) and anti-inflammatory (CD206, MMP9, HGF) markers, as well as the levels of estrogen receptor α, expressed by decidual macrophages in normal pregnancy and in patients with early- and late-onset preeclampsia. The tissue samples of decidua basalis were examined by immunohistochemistry and Western blotting. Isolation of decidual macrophages and their characterization were performed using cultural methods, flow cytometry and real-time PCR. Over 50% of the isolated decidual macrophages were positive for the pan-macrophage marker CD68. In the early-onset preeclampsia group, the levels of estrogen receptor α in decidua were significantly decreased. Furthermore, significantly decreased levels of HGF and CD206 were observed in both preeclampsia groups compared with the control group. The observed downregulation of estrogen receptor α, HGF and CD206 may contribute to the balance of pro- and anti-inflammatory macrophages and thereby to pathogenesis of preeclampsia. |
topic |
macrophages, preeclampsia, decidua, estrogen receptors, polarization, inflammation |
url |
https://www.mdpi.com/2227-9059/9/2/191 |
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