Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells.
Intrinsically photosensitive retinal ganglion cells (ipRGCs) are involved in circadian and other non-image forming visual responses. An open question is whether the activity of these neurons may also be under the regulation mediated by the neurohormone melatonin. In the present work, by double-stain...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4340921?pdf=render |
id |
doaj-12a495cf62b747739ba79c2ed2014bfc |
---|---|
record_format |
Article |
spelling |
doaj-12a495cf62b747739ba79c2ed2014bfc2020-11-25T01:24:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011796710.1371/journal.pone.0117967Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells.Wen-Long ShengWei-Yi ChenXiong-Li YangYong-Mei ZhongShi-Jun WengIntrinsically photosensitive retinal ganglion cells (ipRGCs) are involved in circadian and other non-image forming visual responses. An open question is whether the activity of these neurons may also be under the regulation mediated by the neurohormone melatonin. In the present work, by double-staining immunohistochemical technique, we studied the expression of MT1 and MT2, two known subtypes of mammalian melatonin receptors, in rat ipRGCs. A single subset of retinal ganglion cells labeled by the specific antibody against melanopsin exhibited the morphology typical of M1-type ipRGCs. Immunoreactivity for both MT1 and MT2 receptors was clearly seen in the cytoplasm of all labeled ipRGCs, indicating that these two receptors were co-expressed in each of these neurons. Furthermore, labeling for both the receptors were found in neonatal M1 cells as early as the day of birth. It is therefore highly plausible that retinal melatonin may directly modulate the activity of ipRGCs, thus regulating non-image forming visual functions.http://europepmc.org/articles/PMC4340921?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-Long Sheng Wei-Yi Chen Xiong-Li Yang Yong-Mei Zhong Shi-Jun Weng |
spellingShingle |
Wen-Long Sheng Wei-Yi Chen Xiong-Li Yang Yong-Mei Zhong Shi-Jun Weng Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells. PLoS ONE |
author_facet |
Wen-Long Sheng Wei-Yi Chen Xiong-Li Yang Yong-Mei Zhong Shi-Jun Weng |
author_sort |
Wen-Long Sheng |
title |
Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells. |
title_short |
Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells. |
title_full |
Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells. |
title_fullStr |
Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells. |
title_full_unstemmed |
Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells. |
title_sort |
co-expression of two subtypes of melatonin receptor on rat m1-type intrinsically photosensitive retinal ganglion cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Intrinsically photosensitive retinal ganglion cells (ipRGCs) are involved in circadian and other non-image forming visual responses. An open question is whether the activity of these neurons may also be under the regulation mediated by the neurohormone melatonin. In the present work, by double-staining immunohistochemical technique, we studied the expression of MT1 and MT2, two known subtypes of mammalian melatonin receptors, in rat ipRGCs. A single subset of retinal ganglion cells labeled by the specific antibody against melanopsin exhibited the morphology typical of M1-type ipRGCs. Immunoreactivity for both MT1 and MT2 receptors was clearly seen in the cytoplasm of all labeled ipRGCs, indicating that these two receptors were co-expressed in each of these neurons. Furthermore, labeling for both the receptors were found in neonatal M1 cells as early as the day of birth. It is therefore highly plausible that retinal melatonin may directly modulate the activity of ipRGCs, thus regulating non-image forming visual functions. |
url |
http://europepmc.org/articles/PMC4340921?pdf=render |
work_keys_str_mv |
AT wenlongsheng coexpressionoftwosubtypesofmelatoninreceptoronratm1typeintrinsicallyphotosensitiveretinalganglioncells AT weiyichen coexpressionoftwosubtypesofmelatoninreceptoronratm1typeintrinsicallyphotosensitiveretinalganglioncells AT xiongliyang coexpressionoftwosubtypesofmelatoninreceptoronratm1typeintrinsicallyphotosensitiveretinalganglioncells AT yongmeizhong coexpressionoftwosubtypesofmelatoninreceptoronratm1typeintrinsicallyphotosensitiveretinalganglioncells AT shijunweng coexpressionoftwosubtypesofmelatoninreceptoronratm1typeintrinsicallyphotosensitiveretinalganglioncells |
_version_ |
1725117999629729792 |