Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice.
Mutations in the LMNA gene, which encodes all A-type lamins, result in a variety of human diseases termed laminopathies. Lmna(-/-) mice appear normal at birth but become runted as early as 2 weeks of age and develop multiple tissue defects that mimic some aspects of human laminopathies. Lmna(-/-) mi...
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doaj-12b4a4da4a4c4832bea0f44d969598a92020-11-25T02:33:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-04-0154e1012710.1371/journal.pone.0010127Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice.J Scott HaleRichard L FrockSara A MammanPamela J FinkBrian K KennedyMutations in the LMNA gene, which encodes all A-type lamins, result in a variety of human diseases termed laminopathies. Lmna(-/-) mice appear normal at birth but become runted as early as 2 weeks of age and develop multiple tissue defects that mimic some aspects of human laminopathies. Lmna(-/-) mice also display smaller spleens and thymuses. In this study, we investigated whether altered lymphoid organ sizes are correlated with specific defects in lymphocyte development.Lmna(-/-) mice displayed severe age-dependent defects in T and B cell development which coincided with runting. Lmna(-/-) bone marrow reconstituted normal T and B cell development in irradiated wild-type recipients, driving generation of functional and self-MHC restricted CD4(+) and CD8(+) T cells. Transplantation of Lmna(-/-) neonatal thymus lobes into syngeneic wild-type recipients resulted in good engraftment of thymic tissue and normal thymocyte development.Collectively, these data demonstrate that the severe defects in lymphocyte development that characterize Lmna(-/-) mice do not result directly from the loss of A-type lamin function in lymphocytes or thymic stroma. Instead, the immune defects in Lmna(-/-) mice likely reflect indirect damage, perhaps resulting from prolonged stress due to the striated muscle dystrophies that occur in these mice.http://europepmc.org/articles/PMC2853576?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J Scott Hale Richard L Frock Sara A Mamman Pamela J Fink Brian K Kennedy |
spellingShingle |
J Scott Hale Richard L Frock Sara A Mamman Pamela J Fink Brian K Kennedy Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice. PLoS ONE |
author_facet |
J Scott Hale Richard L Frock Sara A Mamman Pamela J Fink Brian K Kennedy |
author_sort |
J Scott Hale |
title |
Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice. |
title_short |
Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice. |
title_full |
Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice. |
title_fullStr |
Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice. |
title_full_unstemmed |
Cell-extrinsic defective lymphocyte development in Lmna(-/-) mice. |
title_sort |
cell-extrinsic defective lymphocyte development in lmna(-/-) mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-04-01 |
description |
Mutations in the LMNA gene, which encodes all A-type lamins, result in a variety of human diseases termed laminopathies. Lmna(-/-) mice appear normal at birth but become runted as early as 2 weeks of age and develop multiple tissue defects that mimic some aspects of human laminopathies. Lmna(-/-) mice also display smaller spleens and thymuses. In this study, we investigated whether altered lymphoid organ sizes are correlated with specific defects in lymphocyte development.Lmna(-/-) mice displayed severe age-dependent defects in T and B cell development which coincided with runting. Lmna(-/-) bone marrow reconstituted normal T and B cell development in irradiated wild-type recipients, driving generation of functional and self-MHC restricted CD4(+) and CD8(+) T cells. Transplantation of Lmna(-/-) neonatal thymus lobes into syngeneic wild-type recipients resulted in good engraftment of thymic tissue and normal thymocyte development.Collectively, these data demonstrate that the severe defects in lymphocyte development that characterize Lmna(-/-) mice do not result directly from the loss of A-type lamin function in lymphocytes or thymic stroma. Instead, the immune defects in Lmna(-/-) mice likely reflect indirect damage, perhaps resulting from prolonged stress due to the striated muscle dystrophies that occur in these mice. |
url |
http://europepmc.org/articles/PMC2853576?pdf=render |
work_keys_str_mv |
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