Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation
Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms...
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doaj-12d75bfad8e94031ab23feffcf111f862020-11-25T00:45:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-07-01187160310.3390/ijms18071603ijms18071603Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent ActivationAnne Louise Schacht Revenfeld0Rikke Bæk1Malene Møller Jørgensen2Kim Varming3Allan Stensballe4Department of Clinical Immunology, Aalborg University Hospital, Urbansgade 32-36, DK-9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, Urbansgade 32-36, DK-9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, Urbansgade 32-36, DK-9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, Urbansgade 32-36, DK-9000 Aalborg, DenmarkDepartment of Health Science and Technology, Aalborg University, Laboratory for Medical Mass Spectrometry, Fredrik Bajersvej 7E, 9100 Aalborg, DenmarkAlthough the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed lymphocyte culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication.https://www.mdpi.com/1422-0067/18/7/1603peripheral toleranceregulatory T cellsflow cytometryEV Array |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anne Louise Schacht Revenfeld Rikke Bæk Malene Møller Jørgensen Kim Varming Allan Stensballe |
spellingShingle |
Anne Louise Schacht Revenfeld Rikke Bæk Malene Møller Jørgensen Kim Varming Allan Stensballe Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation International Journal of Molecular Sciences peripheral tolerance regulatory T cells flow cytometry EV Array |
author_facet |
Anne Louise Schacht Revenfeld Rikke Bæk Malene Møller Jørgensen Kim Varming Allan Stensballe |
author_sort |
Anne Louise Schacht Revenfeld |
title |
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation |
title_short |
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation |
title_full |
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation |
title_fullStr |
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation |
title_full_unstemmed |
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation |
title_sort |
induction of a regulatory phenotype in cd3+ cd4+ hla-dr+ t cells after allogeneic mixed lymphocyte culture; indications of both contact-dependent and -independent activation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-07-01 |
description |
Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed lymphocyte culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication. |
topic |
peripheral tolerance regulatory T cells flow cytometry EV Array |
url |
https://www.mdpi.com/1422-0067/18/7/1603 |
work_keys_str_mv |
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