Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens
Abstract An experiment was conducted to investigate the effects of hydroalcoholic extract of Withania coagulans (WC) fruit and 1.25-dihydroxycholecalciferol (1.25-(OH)2 D3) on bone mineralization, mechanical and histological properties of male broiler chickens at 21 and 42 d of age. A total of six h...
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2016-03-01
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doaj-13142bd587814f49b5ee37ab506584462020-11-24T23:56:28ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612016-03-01181738610.1590/18069061-2015-0016S1516-635X2016000100073Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler ChickensSJ HosseiniH KermanshahiH NassirimoghaddamA NabipourMT MirakzehH SalehM KazemifardAbstract An experiment was conducted to investigate the effects of hydroalcoholic extract of Withania coagulans (WC) fruit and 1.25-dihydroxycholecalciferol (1.25-(OH)2 D3) on bone mineralization, mechanical and histological properties of male broiler chickens at 21 and 42 d of age. A total of six hundred male day-old Ross 308 broiler chickens were randomly distributed according to a completely randomized experimental design in a 2×3×2 factorial arrangement with 12 treatments of five replicates of 10 birds each. Treatments consisted of two basal diets (positive control with adequate Ca level and negative control with 30% less Ca), three levels of WC (0, 100, or 200 mg/kg diet), and two levels of 1.25-(OH)2 D3 (0 or 0.5 µg/kg diet). Birds were housed in floor pens. The diets were fed ad libitum from one to 42 days of age. On day 21 and 42, one bird per replicate was sacrificed and its tibiae were removed. Both Ca and P retention increased when dietary Ca level was reduced (p<0.001). The addition of 200 mg WC/kg to positive control diet increased Ca retention (p<0.01). Except for tibia diameter, no significant main effects of experimental treatments were observed on tibia physical characteristics or on bone mineralization. The diet with 30% Ca reduction decreased tibia diameter at 42 days of age (p<0.05). The dietary addition of 1.25-(OH)2 D3 increased tibia fracture energy, width of tibia mineralized zone, and serum Ca at 42 days of age (p<0.05). At 21 days of age, supplementation of 100 mg WC/kg increased cortical thickness (p<0.05). At 42 days of age, supplementation of 100 mg WC/kg increased tibia shear force (p<0.05) and fracture energy (p<0.01). The results of this experiment showed that supplementation of 100 mg/kg hydroalcoholic extract of WC fruit increased tibia cortical thickness, shear force, and fracture energy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100073&lng=en&tlng=en1.25-dihydroxycholecalciferolWithania coagulansbonebroiler chickens |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
SJ Hosseini H Kermanshahi H Nassirimoghaddam A Nabipour MT Mirakzeh H Saleh M Kazemifard |
spellingShingle |
SJ Hosseini H Kermanshahi H Nassirimoghaddam A Nabipour MT Mirakzeh H Saleh M Kazemifard Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens Brazilian Journal of Poultry Science 1.25-dihydroxycholecalciferol Withania coagulans bone broiler chickens |
author_facet |
SJ Hosseini H Kermanshahi H Nassirimoghaddam A Nabipour MT Mirakzeh H Saleh M Kazemifard |
author_sort |
SJ Hosseini |
title |
Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens |
title_short |
Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens |
title_full |
Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens |
title_fullStr |
Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens |
title_full_unstemmed |
Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens |
title_sort |
effects of 1.25-dihydroxycholecalciferol and hydroalcoholic extract of withania coagulans fruit on bone mineralization and mechanical and histological properties of male broiler chickens |
publisher |
Fundação APINCO de Ciência e Tecnologia Avícolas |
series |
Brazilian Journal of Poultry Science |
issn |
1806-9061 |
publishDate |
2016-03-01 |
description |
Abstract An experiment was conducted to investigate the effects of hydroalcoholic extract of Withania coagulans (WC) fruit and 1.25-dihydroxycholecalciferol (1.25-(OH)2 D3) on bone mineralization, mechanical and histological properties of male broiler chickens at 21 and 42 d of age. A total of six hundred male day-old Ross 308 broiler chickens were randomly distributed according to a completely randomized experimental design in a 2×3×2 factorial arrangement with 12 treatments of five replicates of 10 birds each. Treatments consisted of two basal diets (positive control with adequate Ca level and negative control with 30% less Ca), three levels of WC (0, 100, or 200 mg/kg diet), and two levels of 1.25-(OH)2 D3 (0 or 0.5 µg/kg diet). Birds were housed in floor pens. The diets were fed ad libitum from one to 42 days of age. On day 21 and 42, one bird per replicate was sacrificed and its tibiae were removed. Both Ca and P retention increased when dietary Ca level was reduced (p<0.001). The addition of 200 mg WC/kg to positive control diet increased Ca retention (p<0.01). Except for tibia diameter, no significant main effects of experimental treatments were observed on tibia physical characteristics or on bone mineralization. The diet with 30% Ca reduction decreased tibia diameter at 42 days of age (p<0.05). The dietary addition of 1.25-(OH)2 D3 increased tibia fracture energy, width of tibia mineralized zone, and serum Ca at 42 days of age (p<0.05). At 21 days of age, supplementation of 100 mg WC/kg increased cortical thickness (p<0.05). At 42 days of age, supplementation of 100 mg WC/kg increased tibia shear force (p<0.05) and fracture energy (p<0.01). The results of this experiment showed that supplementation of 100 mg/kg hydroalcoholic extract of WC fruit increased tibia cortical thickness, shear force, and fracture energy. |
topic |
1.25-dihydroxycholecalciferol Withania coagulans bone broiler chickens |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100073&lng=en&tlng=en |
work_keys_str_mv |
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