Drosophila Models Rediscovered with Super-Resolution Microscopy
With the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjec...
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doaj-13361c5b00a44b55b0ebc24924a1145f2021-08-26T13:37:06ZengMDPI AGCells2073-44092021-07-01101924192410.3390/cells10081924Drosophila Models Rediscovered with Super-Resolution MicroscopySzilárd Szikora0Péter Görög1Csaba Kozma2József Mihály3Institute of Genetics, Biological Research Centre, Temesvári krt. 62, H-6726 Szeged, HungaryInstitute of Genetics, Biological Research Centre, Temesvári krt. 62, H-6726 Szeged, HungaryFoundation for the Future of Biomedical Sciences in Szeged, Szeged Scientists Academy, Pálfy u. 52/d, H-6725 Szeged, HungaryInstitute of Genetics, Biological Research Centre, Temesvári krt. 62, H-6726 Szeged, HungaryWith the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjects because combining the strength of sophisticated genetics, biochemistry and electrophysiology with the unparalleled resolution provided by super-resolution imaging appears as one of the most efficient approaches to understanding the basic cell biological questions and the molecular complexity of life. Here, we summarize the major nanoscopic techniques and illustrate how these approaches were used in <i>Drosophila</i> model systems to revisit a series of well-known cell biological phenomena. These investigations clearly demonstrate that instead of simply achieving an improvement in image quality, nanoscopy goes far beyond with its immense potential to discover novel structural and mechanistic aspects. With the examples of synaptic active zones, centrosomes and sarcomeres, we will explain the instrumental role of super-resolution imaging pioneered in <i>Drosophila</i> in understanding fundamental subcellular constituents.https://www.mdpi.com/2073-4409/10/8/1924nanoscopysuper-resolution microscopySIMSTEDSMLM<i>Drosophila</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Szilárd Szikora Péter Görög Csaba Kozma József Mihály |
spellingShingle |
Szilárd Szikora Péter Görög Csaba Kozma József Mihály Drosophila Models Rediscovered with Super-Resolution Microscopy Cells nanoscopy super-resolution microscopy SIM STED SMLM <i>Drosophila</i> |
author_facet |
Szilárd Szikora Péter Görög Csaba Kozma József Mihály |
author_sort |
Szilárd Szikora |
title |
Drosophila Models Rediscovered with Super-Resolution Microscopy |
title_short |
Drosophila Models Rediscovered with Super-Resolution Microscopy |
title_full |
Drosophila Models Rediscovered with Super-Resolution Microscopy |
title_fullStr |
Drosophila Models Rediscovered with Super-Resolution Microscopy |
title_full_unstemmed |
Drosophila Models Rediscovered with Super-Resolution Microscopy |
title_sort |
drosophila models rediscovered with super-resolution microscopy |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-07-01 |
description |
With the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjects because combining the strength of sophisticated genetics, biochemistry and electrophysiology with the unparalleled resolution provided by super-resolution imaging appears as one of the most efficient approaches to understanding the basic cell biological questions and the molecular complexity of life. Here, we summarize the major nanoscopic techniques and illustrate how these approaches were used in <i>Drosophila</i> model systems to revisit a series of well-known cell biological phenomena. These investigations clearly demonstrate that instead of simply achieving an improvement in image quality, nanoscopy goes far beyond with its immense potential to discover novel structural and mechanistic aspects. With the examples of synaptic active zones, centrosomes and sarcomeres, we will explain the instrumental role of super-resolution imaging pioneered in <i>Drosophila</i> in understanding fundamental subcellular constituents. |
topic |
nanoscopy super-resolution microscopy SIM STED SMLM <i>Drosophila</i> |
url |
https://www.mdpi.com/2073-4409/10/8/1924 |
work_keys_str_mv |
AT szilardszikora drosophilamodelsrediscoveredwithsuperresolutionmicroscopy AT petergorog drosophilamodelsrediscoveredwithsuperresolutionmicroscopy AT csabakozma drosophilamodelsrediscoveredwithsuperresolutionmicroscopy AT jozsefmihaly drosophilamodelsrediscoveredwithsuperresolutionmicroscopy |
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