Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis
SETTING: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. OBJECTIVE: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. METHOD: during one year, sputum samples were collected from 72 pulmonary tu...
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doaj-1358de32ef5b4cff87550f67bb6158ea2020-11-25T03:20:47ZengElsevierBrazilian Journal of Infectious Diseases1678-439114111612010.1590/S1413-86702010000100022S1413-86702010000100022Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosisRosemeri Maurici da Silva0Maria Luiza Bazzo1Mariana Chagas2Universidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaSETTING: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. OBJECTIVE: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. METHOD: during one year, sputum samples were collected from 72 pulmonary tuberculosis patients and 12 non-tuberculosis controls, which were admitted to the Nereu Ramos hospital, Florianópolis city, Brazil. The samples were subjected to Ziehl-Neelsen-stained sputum smear microscopy and Lowestein-Jensen medium culture, which were defined as gold standard tests for mycobacteria, and polymerase chain reaction (PCR). Those samples that presented more than 40% of viable cells and less than 25% of epithelial cells were defined as high quality samples. RESULTS: PCR showed sensitivity of 55.6%, specificity of 41.7%, positive predictive value of 85.1%, negative predictive value of 13.5%, and accuracy of 53.6%. High quality samples showed sensitivity of 72.4%, specificity of 50%, positive predictive value of 91.3%, negative predictive value of 20%, and accuracy of 69.7%. Low quality samples showed sensitivity of 44.2%, specificity of 37.5%, positive predictive value of 79.2%, negative predictive value of 11.1%, and accuracy of 43.1%. CONCLUSION: use of high quality samples improved significantly the PCR performance, especially on their sensitivity and positive predictive values.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100022&lng=en&tlng=entuberculosissputumpolymerase chain reaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rosemeri Maurici da Silva Maria Luiza Bazzo Mariana Chagas |
spellingShingle |
Rosemeri Maurici da Silva Maria Luiza Bazzo Mariana Chagas Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis Brazilian Journal of Infectious Diseases tuberculosis sputum polymerase chain reaction |
author_facet |
Rosemeri Maurici da Silva Maria Luiza Bazzo Mariana Chagas |
author_sort |
Rosemeri Maurici da Silva |
title |
Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
title_short |
Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
title_full |
Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
title_fullStr |
Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
title_full_unstemmed |
Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
title_sort |
quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1678-4391 |
description |
SETTING: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. OBJECTIVE: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. METHOD: during one year, sputum samples were collected from 72 pulmonary tuberculosis patients and 12 non-tuberculosis controls, which were admitted to the Nereu Ramos hospital, Florianópolis city, Brazil. The samples were subjected to Ziehl-Neelsen-stained sputum smear microscopy and Lowestein-Jensen medium culture, which were defined as gold standard tests for mycobacteria, and polymerase chain reaction (PCR). Those samples that presented more than 40% of viable cells and less than 25% of epithelial cells were defined as high quality samples. RESULTS: PCR showed sensitivity of 55.6%, specificity of 41.7%, positive predictive value of 85.1%, negative predictive value of 13.5%, and accuracy of 53.6%. High quality samples showed sensitivity of 72.4%, specificity of 50%, positive predictive value of 91.3%, negative predictive value of 20%, and accuracy of 69.7%. Low quality samples showed sensitivity of 44.2%, specificity of 37.5%, positive predictive value of 79.2%, negative predictive value of 11.1%, and accuracy of 43.1%. CONCLUSION: use of high quality samples improved significantly the PCR performance, especially on their sensitivity and positive predictive values. |
topic |
tuberculosis sputum polymerase chain reaction |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100022&lng=en&tlng=en |
work_keys_str_mv |
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