Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy

In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella t...

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Main Authors: Yingxue Ren, Daniel L. Vera, Kimberly A. Hughes, Jonathan H. Dennis
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Genomics Data
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2213596015000021
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spelling doaj-136b24a920b540d0a91d290f18cdc9cd2020-11-25T03:35:28ZengElsevierGenomics Data2213-59602015-03-013C14614710.1016/j.gdata.2015.01.001Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancyYingxue RenDaniel L. VeraKimberly A. HughesJonathan H. DennisIn eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4 h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.http://www.sciencedirect.com/science/article/pii/S2213596015000021NucleosomeDrosophilaSalmonellaMNaseIllumina Hi-Seq
collection DOAJ
language English
format Article
sources DOAJ
author Yingxue Ren
Daniel L. Vera
Kimberly A. Hughes
Jonathan H. Dennis
spellingShingle Yingxue Ren
Daniel L. Vera
Kimberly A. Hughes
Jonathan H. Dennis
Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
Genomics Data
Nucleosome
Drosophila
Salmonella
MNase
Illumina Hi-Seq
author_facet Yingxue Ren
Daniel L. Vera
Kimberly A. Hughes
Jonathan H. Dennis
author_sort Yingxue Ren
title Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
title_short Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
title_full Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
title_fullStr Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
title_full_unstemmed Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
title_sort stimulation of the drosophila immune system alters genome-wide nucleosome occupancy
publisher Elsevier
series Genomics Data
issn 2213-5960
publishDate 2015-03-01
description In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4 h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.
topic Nucleosome
Drosophila
Salmonella
MNase
Illumina Hi-Seq
url http://www.sciencedirect.com/science/article/pii/S2213596015000021
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