Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy
In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella t...
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doaj-136b24a920b540d0a91d290f18cdc9cd2020-11-25T03:35:28ZengElsevierGenomics Data2213-59602015-03-013C14614710.1016/j.gdata.2015.01.001Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancyYingxue RenDaniel L. VeraKimberly A. HughesJonathan H. DennisIn eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4 h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.http://www.sciencedirect.com/science/article/pii/S2213596015000021NucleosomeDrosophilaSalmonellaMNaseIllumina Hi-Seq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yingxue Ren Daniel L. Vera Kimberly A. Hughes Jonathan H. Dennis |
spellingShingle |
Yingxue Ren Daniel L. Vera Kimberly A. Hughes Jonathan H. Dennis Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy Genomics Data Nucleosome Drosophila Salmonella MNase Illumina Hi-Seq |
author_facet |
Yingxue Ren Daniel L. Vera Kimberly A. Hughes Jonathan H. Dennis |
author_sort |
Yingxue Ren |
title |
Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy |
title_short |
Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy |
title_full |
Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy |
title_fullStr |
Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy |
title_full_unstemmed |
Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy |
title_sort |
stimulation of the drosophila immune system alters genome-wide nucleosome occupancy |
publisher |
Elsevier |
series |
Genomics Data |
issn |
2213-5960 |
publishDate |
2015-03-01 |
description |
In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4 h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507. |
topic |
Nucleosome Drosophila Salmonella MNase Illumina Hi-Seq |
url |
http://www.sciencedirect.com/science/article/pii/S2213596015000021 |
work_keys_str_mv |
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